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Add To Compare List Mouse Nucleolar Antigen Monoclonal Antibody Catalog # MBS438004 Unit / Price 0.02 mg (With BSA & Azide at 0.2mg/mL) / $240 +1 FREE 8GB USB 0.1 mg (With BSA & Azide at 0.2mg/mL) / $415 +1 FREE 8GB USB 0.1 mg (Without BSA & Azide at 1mg/mL) / $415 +1 FREE 8GB USB 5x0.1 mg (With BSA & Azide at 0.2mg/mL) / $1,735 +2 FREE 8GB USB 5x0.1 mg (Without BSA & Azide at 1mg/mL) / $1,735 +2 FREE 8GB USB Previous Next Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Nucleolar Antigen, Monoclonal Antibody Popular Item Full Product Name Human Nucleolar Antigen (Marker For Human Cells) Mouse Monoclonal Antibody Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Monoclonal Isotype IgG1, kappa Clone Number NM95 Host Mouse Species Reactivity Human.Does not react with Mouse, Rat and Cow Specificity This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb NM95 recognizes an antigen associated with the nucleoli in human cells. It can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections. Form/Format 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. Cellular Localization Nucleoli Immunogen Nuclei of myeloid leukemia biopsy cells Positive Control Any human cells. Tonsil Preparation and Storage Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required. Other Notes Small volumes of anti-Nucleolar Antigen antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Applications Tested/Suitable for anti-Nucleolar Antigen antibody Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin Application Notes for anti-Nucleolar Antigen antibody Immunofluorescence (0.5-1.0ug/ml)Immunocytochemistry (Acetone-fixed cells) (0.5-1.0ug/ml for 30 minutes at RT)Immunohistochemistry (Formalin-paraffin) (0.25-0.5ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Immunohistochemistry (IHC) of anti-Nucleolar Antigen antibody Formalin-fixed, paraffin-embedded human Skin stained with Nucleolar Monoclonal Antibody (NM95). Immunohistochemistry (IHC) of anti-Nucleolar Antigen antibody Formalin-fixed, paraffin-embedded human Tonsil stained with Nucleolar Monoclonal Antibody (NM95). Immunohistochemistry (IHC) of anti-Nucleolar Antigen antibody Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with AF488 Conjugate of Nucleolar Monoclonal Antibody (NM95). Flow Cytometry (FC/FACS of anti-Nucleolar Antigen antibody Flow Cytometry of Human Nucleolar Ag on 293T Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled human Nucleolar Monoclonal Antibody (NM95). TOP Product References and Citations for anti-Nucleolar Antigen antibody Epstein, A.L. and Clevenger, C.V., Identification of nuclear antigens in human cells by immunofluorescence, immunoelectron microscopy, and immuno-biochemical methods using monoclonal antibodies. In Progress on nonhistone protein research, Vol. 1, Isaac Bekhor, ed., 1985, CRC Press, Boca Raton, FL, pp 117-137 TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP