View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Add To Compare List anti-O-GalNAc antibody :: Mouse O-GalNAc Monoclonal Antibody Catalog # MBS808562 Unit / Price DISCONTINUEDClick here to view our similar product(s) OR click here to contact our technical support team for suitable replacement. Previous Next Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name O-GalNAc, Monoclonal Antibody Full Product Name O-GalNAc Antibody, Clone 9B9: ATTO 655 Product Synonym Names Mouse Anti-beta-O-Linked N-Acetylgalactosamine Monoclonal IgM; O-GalNAc Antibody: ATTO 655; GalNAC Antibody; O-GalNAC Antibody; beta-O-Linked N-Acetylgalactosamine Antibody; O-Linked N-Acetylgalactosamine Antibody; N-Acetylgalactosamine Antibody; 2-(Acetylamino)-2-deoxy-D-galactose Antibody; 2-Acetamido-2-deoxy-D-galactose Antibody; N-Acetylchondrosamine Antibody; 2-Acetamido-2-deoxy-D-galactopyranose Antibody; N-Acetyl-D-galactosamine Antibody; alpha-N-Acetylgalactosamine Antibody; N-Acetylgalactosamine-modified protein Antibody Product Gene Name anti-O-GalNAc antibody[Similar Products] Matching Pairs Unconjugated Antibody: O-GalNAc Clone #9B9 (MBS808556)ATTO 655 Conjugated Antibody: O-GalNAc Clone #9B9 (MBS808562) Matching Pairs Unconjugated Antibody: N/A (MBS808562)ATTO 655 Conjugated Antibody: O-GalNAc Clone #9B9 (MBS808562) Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Monoclonal Isotype IgM Clone Number 9B9 Host Mouse Species Reactivity Species Independent Specificity Specific for beta-O-Linked N-Acetylgalactosamine and beta-O-Linked N-Acetylglucosamine modified proteins. Does not cross-react with Glucose or Galactose modified proteins. Purity/Purification PEG Purified Concentration 1 mg/ml (lot specific) Target O-GalNAC Immunogen Synthetic beta-O-Linked N-Acetylglucosamine conjugated to Keyhole Limpet Hemocyanin (KLH). Conjugate ATTO 655 Storage Buffer PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide Preparation and Storage Store at -20 degree C. Other Notes Small volumes of anti-O-GalNAc antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Product Categories/Family for anti-O-GalNAc antibody Antibodies; Cell Signaling; Post-translational Modifications; Glycation Applications Tested/Suitable for anti-O-GalNAc antibody Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA) Application Notes for anti-O-GalNAc antibody WB: 1:1000ICC/IF: 1:50ELISA: 1:1000 Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-O-GalNAc antibody Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GalNAC Monoclonal Antibody, Clone 9B9. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GalNAC Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) O-GalNAc Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria. Western Blot (WB) of anti-O-GalNAc antibody Western Blot analysis of Glycoconjugates showing detection of 67 kDa O-GalNAC protein using Mouse Anti-O-GalNAC Monoclonal Antibody, Clone 9B9. Lane 1: Molecular Weight Ladder (MW). Lane 2: GlcNAc-BSA. Lane 3: GalNAc-BSA. Lane 4: Galactose-BSA. Lane 5: Glucose-BSA. Load: 2.0 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-O-GalNAC Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. 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