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Add To Compare List anti-O-GalNAc antibody :: Mouse O-GalNAc Monoclonal Antibody Catalog # MBS808565 Unit / Price 0.1 mg / $570 +1 FREE 8GB USB 5x0.1 mg / $2,265 +2 FREE 8GB USB Previous Next Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name O-GalNAc, Monoclonal Antibody Popular Item Full Product Name O-GalNAc Antibody, Clone 9B9: Alkaline Phosphatase Product Synonym Names Mouse Anti-beta-O-Linked N-Acetylgalactosamine Monoclonal IgM; O-GalNAc Antibody: Alkaline Phosphatase; GalNAC Antibody; O-GalNAC Antibody; beta-O-Linked N-Acetylgalactosamine Antibody; O-Linked N-Acetylgalactosamine Antibody; N-Acetylgalactosamine Antibody; 2-(Acetylamino)-2-deoxy-D-galactose Antibody; 2-Acetamido-2-deoxy-D-galactose Antibody; N-Acetylchondrosamine Antibody; 2-Acetamido-2-deoxy-D-galactopyranose Antibody; N-Acetyl-D-galactosamine Antibody; alpha-N-Acetylgalactosamine Antibody; N-Acetylgalactosamine-modified protein Antibody Product Gene Name anti-O-GalNAc antibody[Similar Products] Matching Pairs Unconjugated Antibody: O-GalNAc Clone #9B9 (MBS808556)Alkaline Phosphatase Conjugated Antibody: O-GalNAc Clone #9B9 (MBS808565) Matching Pairs Unconjugated Antibody: N/A (MBS808565)Alkaline Phosphatase Conjugated Antibody: O-GalNAc Clone #9B9 (MBS808565) Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Monoclonal Isotype IgM Clone Number 9B9 Host Mouse Species Reactivity Species Independent Specificity Specific for beta-O-Linked N-Acetylgalactosamine and beta-O-Linked N-Acetylglucosamine modified proteins. Does not cross-react with Glucose or Galactose modified proteins. Purity/Purification PEG Purified Concentration 1 mg/ml (lot specific) Immunogen Synthetic beta-O-Linked N-Acetylglucosamine conjugated to Keyhole Limpet Hemocyanin (KLH). Conjugate Alkaline Phosphatase Storage Buffer PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide Certificate of Analysis A 1:1000 dilution of MBS808565 was sufficient for detection of O-GlcNAc in 2 ug of GlcNAc conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG: HRP as the secondary antibody. Preparation and Storage Store at -20°C for 1 year. Avoid freeze/ thaw cycles. Other Notes Small volumes of anti-O-GalNAc antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Product Categories/Family for anti-O-GalNAc antibody Antibodies; Cell Signaling; Post-translational Modifications; Glycation Applications Tested/Suitable for anti-O-GalNAc antibody Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA) Application Notes for anti-O-GalNAc antibody WB: 1:1000)ICC/IF (1:50)ELISA (1:1000)Optimal dilutions for assays should be determined by the user. Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-O-GalNAc antibody Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GalNAC Monoclonal Antibody, Clone 9B9. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GalNAC Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) O-GalNAc Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria. Western Blot (WB) of anti-O-GalNAc antibody Western Blot analysis of Glycoconjugates showing detection of 67 kDa O-GalNAC protein using Mouse Anti-O-GalNAC Monoclonal Antibody, Clone 9B9. Lane 1: Molecular Weight Ladder (MW). Lane 2: GlcNAc-BSA. Lane 3: GalNAc-BSA. Lane 4: Galactose-BSA. Lane 5: Glucose-BSA. Load: 2.0 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-O-GalNAC Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP