View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Add To Compare List anti-O-GlcNAc antibody :: Mouse O-GlcNAc Monoclonal Antibody Catalog # MBS808543 Unit / Price DISCONTINUEDClick here to view our similar product(s) OR click here to contact our technical support team for suitable replacement. Previous Next Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name O-GlcNAc, Monoclonal Antibody Full Product Name O-GlcNAc Antibody, Clone 9H6: ATTO 633 Product Synonym Names Mouse Anti-beta-O-Linked N-Acetylglucosamine Monoclonal IgM; beta-O-Linked N-Acetylglucosamine Antibody; O-Linked N-Acetylglucosamine Antibody; O-GlcNAc Antibody; Beta-O-Linked N-Acetylglucosamine Antibody; N-Acetylglucosamine Antibody; GlcNac Antibody; N-Acetylglucosamine Antibody; 2-(Acetylamino)-2-deoxy-D-glucose Antibody; N-acetyl-D-glucosamine Antibody; NAG Antibody; beta-D-(Acetylamino)-2-deoxy-glucopyranose Antibody; beta-N-Acetylglucosamine Antibody; N-Acetylglucosamine-modified protein Antibody; beta-N-Acetylglucosamine Antibody Product Gene Name anti-O-GlcNAc antibody[Similar Products] Matching Pairs Unconjugated Antibody: O-GlcNAc Clone #9H6 (MBS808538)ATTO 633 Conjugated Antibody: O-GlcNAc Clone #9H6 (MBS808543) Matching Pairs Unconjugated Antibody: N/A (MBS808543)ATTO 633 Conjugated Antibody: O-GlcNAc Clone #9H6 (MBS808543) Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Monoclonal Isotype IgM Clone Number 9H6 Host Mouse Species Reactivity Species Independent Specificity Specific for beta-O-Linked N-Acetylglucosamine modified proteins. Does not cross-react with N-Acetylgalactosamine, Glucose, or Galactose modified proteins. Purity/Purification PEG Purified Concentration 1 mg/ml (lot specific) Target O-GlcNAc Immunogen Synthetic beta-O-Linked N-Acetylglucosamine conjugated to Keyhole Limpet Hemocyanin (KLH). Conjugate ATTO 633 Storage Buffer PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide Preparation and Storage Store at -20 degree C. Other Notes Small volumes of anti-O-GlcNAc antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Product Categories/Family for anti-O-GlcNAc antibody Antibodies; Cell Signaling; Post-translational Modifications; Glycation Applications Tested/Suitable for anti-O-GlcNAc antibody Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA) Application Notes for anti-O-GlcNAc antibody WB: 1:1000ICC/IF: 1:50ELISA: 1:1000 Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-O-GlcNAc antibody Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GlcNAc Monoclonal Antibody, Clone 9H6. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) O-GlcNAc Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria. Western Blot (WB) of anti-O-GlcNAc antibody Western Blot analysis of GlcNAc-BSA Conjugate showing detection of 67 kDa O-GlcNAc protein using Mouse Anti-O-GlcNAc Monoclonal Antibody, Clone 9H6. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: GlcNAc-BSA. Lane 4: GalNAc-BSA. Lane 5: Galactose-BSA. Lane 6: Glucose-BSA. Lane 7: Citrulline-BSA. Load: 2.0 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa. TOP Protein Family O-GlcNAcase Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. 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