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anti-OPN antibody :: Mouse anti-Human Osteopontin Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS1488153
Unit / Price
0.05 mg  /  $185 +1 FREE 8GB USB
0.1 mg  /  $265 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Osteopontin (OPN), Monoclonal Antibody

★Popular Item★
 Also Known As   

Mouse anti-Human Osteopontin monoclonal Antibody

 Product Synonym Names    Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin; BNSP; PSEC0156
 Product Gene Name   

anti-OPN antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    166490
 3D Structure    ModBase 3D Structure for P10451
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 Clonality    Monoclonal
 Isotype    IgG2a
 Host    Mouse
 Species Reactivity    Human
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 Specificity    Specific for human Osteopontin denatured and native forms
 Purity/Purification    >95%, Protein G Purified
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 Storage Buffer    Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
 Conjugate    Non-conjugated
 Immunogen    Recombinant Human Osteopontin
 Santa Cruz Alternative    Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-21742 / sc-73631
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 Preparation and Storage    Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of anti-OPN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-OPN antibody

   Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity
 Applications Tested/Suitable for anti-OPN antibody   

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

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 Immunohistochemistry (IHC) of anti-OPN antibody    Immunohistochemical of paraffin-embedded human colon cancer using MBS1488153 at dilution of 1:200
anti-OPN antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-OPN antibody    Immunohistochemical of paraffin-embedded human Kidney tissue using MBS1488153 at dilution of 1:200
anti-OPN antibody Immunohistochemistry (IHC) (IHC) image
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 Western Blot (WB) of anti-OPN antibody    All Lanes Mouse anti human OPN monoclonal antibody at at 2ug/ml
Lane 1:mouse kidney tissue lysate
Secondary Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size: 34.5 kDa
Observed band size: 33,36,34kDa
Additional bands at: 50 kDa.
anti-OPN antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for OPN. It may not necessarily be applicable to this product.
 NCBI GI #    4759166
 NCBI GeneID    6696
 NCBI Accession #    NP_000573.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000582.2 [Other Products]
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 UniProt Primary Accession #    P10451 [Other Products]
 UniProt Secondary Accession #    Q15681; Q15682; Q15683; Q4W597; Q567T5; Q8NBK2; Q96IZ1; B2RDA1 [Other Products]
 UniProt Related Accession #    P10451 [Other Products]
 Molecular Weight    33,843 Da
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 NCBI Official Full Name    osteopontin isoform OPN-b
 NCBI Official Synonym Full Names    secreted phosphoprotein 1
 NCBI Official Symbol    SPP1 [Similar Products]
 NCBI Official Synonym Symbols   
OPN; BNSP; BSPI; ETA-1
[Similar Products]
 NCBI Protein Information    osteopontin
 UniProt Protein Name    Osteopontin
 UniProt Synonym Protein Names   
Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin
 Protein Family    Osteopontin
 UniProt Gene Name    SPP1 [Similar Products]
 UniProt Synonym Gene Names    BNSP; OPN; SPP-1 [Similar Products]
 UniProt Entry Name    OSTP_HUMAN
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 NCBI Summary for OPN    The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
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 UniProt Comments for OPN    osteopontin: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Belongs to the osteopontin family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q22.1

Cellular Component: apical part of cell; cell projection; extracellular region; extracellular space; perinuclear region of cytoplasm

Molecular Function: cytokine activity; extracellular matrix binding; protein binding

Biological Process: biomineral formation; cell adhesion; decidualization; embryo implantation; extracellular matrix disassembly; extracellular matrix organization and biogenesis; inflammatory response; negative regulation of collateral sprouting of intact axon in response to injury; neutrophil chemotaxis; osteoblast differentiation; positive regulation of bone resorption; response to steroid hormone stimulus; response to vitamin D
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 Research Articles on OPN    1. A strong positive correlation between the mRNA expression levels of HIF-2alpha, CA9, VEGF, GLUT-1 and OPN suggests a specific hypoxia-associated profile of mRNA expression in glioblastoma multiforme
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Organs/Tissues associated with anti-OPN antibody
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >1101 publications with OPN and Bone
 Blood Antibodies  >558 publications with OPN and Blood
 Kidney Antibodies  >369 publications with OPN and Kidney
 Vascular Antibodies  >352 publications with OPN and Vascular
 Connective Tissue Antibodies  >339 publications with OPN and Connective Tissue
 Muscle Antibodies  >288 publications with OPN and Muscle
 Bone Marrow Antibodies  >195 publications with OPN and Bone Marrow
 Mouth Antibodies  >186 publications with OPN and Mouth
 Liver Antibodies  >135 publications with OPN and Liver
 Heart Antibodies  >133 publications with OPN and Heart
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