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anti-PINK1 antibody :: Mouse PINK1 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS800448
Western Blot (WB)
Unit / Price
0.1 mg  /  $390 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PINK1, Monoclonal Antibody

★Popular Item★
 Also Known As   

PINK1 Antibody

 Product Synonym Names    Parkinson disease (autosomal recessive) 6; EC 2.7.11.1; PARK6; serine/threonine-protein kinase PINK1 mitochondrial; PTEN-induced putative kinase protein 1; protein kinase BRPK; BRPK; FLJ27236; PTEN induced putative kinase 1; Phosphatase and Tensin Homolog
 Product Gene Name   

anti-PINK1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    608309
 3D Structure    ModBase 3D Structure for Q9BXM7
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 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    S4-15
 Host    Mouse
 Species Reactivity    Human, Rat, Mouse
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 Specificity    Detects ~50kDa
 Form/Format    Protein G Purified
 Concentration    1mg/ml (lot specific)
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 Certificate of Analysis    1ug/mL was sufficient for detection of PINK1 in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
 Immunogen    Fusion protein amino acids 112-496 (cytoplasmic C-terminus) of human PINK1. 82% identical to rate and 81% identical to mouse. >30% identity with DMPK.
 Storage Buffer    PBS pH 7.4, 50% glycerol, 0.1% sodium azide
 Research Area(s)    Neuroscience, Cell Signaling, Post-translational Modifications, Phosphorylation, Cadiovascular system, Heart.
 Cellular Localization    Mitchondrion outer membrane, Cytoplasm.
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 Preparation and Storage    -20 degree C; 1yr+ Avoid freeze/thaw cycle.
 Other Notes    Small volumes of anti-PINK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-PINK1 antibody

   Background Info: Detects a 50 kDa protein corresponding to the molecular weight of PINK1

Scientific Background: PINK1 (PTEN induced putative kinase 1) is a mitochondrial serine/threonine kinase which maintains mitochondrial function/integrity, provides protection against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins, and is involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). PINK1 is synthesized as a 63 kD protein which undergoes proteolyt processing to generate at least two cleaved forms (55 kD and 42 kD). PINK1 and its substrates have been found in the cytosol as well as in different sub-mitochondrial compartments, and according to the recent reports; PINK1 may be targeted to OMM (outer mitochondrial membrane) with its kinase domain facing the cytosol, providing a possible explanation for the observed physical interaction with the cytosolic E3 ubiquitin ligase Parkin. Defective PINK1 may cause alterations in processing, stability, localization and activity as well as binding to substrates/interaction-partners which ultimately leads to differential effects on mitochondrial function and morphology. Mutations in PINK1 are linked to autosomal recessive early onset Parkinson's disease, and are associated with loss of protective function, mitochondrial dysfunction, aggregation of alpha-synuclein, as well as proteasome dysfunction.
 Product Categories/Family for anti-PINK1 antibody    Neuroscience
 Applications Tested/Suitable for anti-PINK1 antibody   

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunofluorescence (IF)

 Application Notes for anti-PINK1 antibody    1:1000 WB
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 Western Blot (WB) of anti-PINK1 antibody    Western Blot analysis of Rat Brain showing detection of ~50 kDa PINK1 protein using Mouse Anti-PINK1 Monoclonal Antibody, Clone S4-15. Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-PINK1 Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~50 kDa.
anti-PINK1 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for PINK1. It may not necessarily be applicable to this product.
 NCBI GI #    14165272
 NCBI GeneID    65018
 NCBI Accession #    NP_115785.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_032409.2 [Other Products]
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 UniProt Primary Accession #    Q9BXM7 [Other Products]
 UniProt Secondary Accession #    Q8N6T9; Q8NBU3; Q96DE4 [Other Products]
 UniProt Related Accession #    Q9BXM7 [Other Products]
 Molecular Weight    30,104 Da [Similar Products]
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 NCBI Official Full Name    serine/threonine-protein kinase PINK1, mitochondrial
 NCBI Official Synonym Full Names    PTEN induced putative kinase 1
 NCBI Official Symbol    PINK1 [Similar Products]
 NCBI Official Synonym Symbols   
BRPK; PARK6
[Similar Products]
 NCBI Protein Information    serine/threonine-protein kinase PINK1, mitochondrial; protein kinase BRPK; PTEN-induced putative kinase protein 1
 UniProt Protein Name    Serine/threonine-protein kinase PINK1, mitochondrial
 UniProt Synonym Protein Names   
BRPK; PTEN-induced putative kinase protein 1
 Protein Family    Serine/threonine-protein kinase
 UniProt Gene Name    PINK1 [Similar Products]
 UniProt Entry Name    PINK1_HUMAN
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 NCBI Summary for PINK1    This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease. [provided by RefSeq, Jul 2008]
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 UniProt Comments for PINK1    PINK1: Protects against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). It is necessary for PARK2 recruitment to dysfunctional mitochondria to initiate their degradation. Interats with PARK2. Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Protein kinase, Other; Kinase, protein; Mitochondrial; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Other group; NKF2 family

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: mitochondrion; mitochondrial intermembrane space; cytosol; mitochondrial outer membrane; cytoskeleton; membrane; axon; perinuclear region of cytoplasm; mitochondrial inner membrane; cytoplasm; TORC2 complex; chromatin; nucleus; integral to mitochondrial outer membrane

Molecular Function: protein kinase B binding; protein serine/threonine kinase activity; protein binding; protease activator activity; protease binding; ubiquitin protein ligase binding; magnesium ion binding; kinase activity; ATP binding

Biological Process: ubiquitin-dependent protein catabolic process; negative regulation of JNK cascade; positive regulation of dopamine secretion; positive regulation of translation; regulation of protein ubiquitination; protein ubiquitination; protein amino acid phosphorylation; positive regulation of protein amino acid dephosphorylation; negative regulation of macroautophagy; regulation of mitochondrial membrane potential; positive regulation of ubiquitin-protein ligase activity; regulation of protein complex assembly; response to stress; negative regulation of neuron apoptosis; regulation of hydrogen peroxide metabolic process; mitochondrion organization and biogenesis; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein stabilization; activation of protein kinase B; positive regulation of synaptic transmission, dopaminergic; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein kinase B signaling cascade; peptidyl-serine phosphorylation; mitochondrion degradation; positive regulation of transcription factor activity; response to oxidative stress; positive regulation of protein amino acid phosphorylation; phosphorylation

Disease: Parkinson Disease 6, Autosomal Recessive Early-onset
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 Research Articles on PINK1    1. PINK1 expression is significantly increased upon CCCP-induced mitophagy in a calcium-dependent manner. PINK1 may play a role in mitophagy that is downstream of ubiquitination of mitochondrial substrates
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-PINK1 antibody
 Products by Pathway  Pathway Diagram
 Parkinson's Disease Pathway antibodies  Parkinson's Disease Pathway Diagram
 Parkinsons Disease Pathway antibodies  Parkinsons Disease Pathway Diagram
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