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anti-PKC-zeta antibody :: Rabbit PKC-zeta Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS684156
Testing Data
Unit / Price
0.02 mL  /  $235 +1 FREE 8GB USB
0.05 mL  /  $335 +1 FREE 8GB USB
0.1 mL  /  $480 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PKC-zeta, Monoclonal Antibody

 Also Known As   

Anti - PKC-zeta

 Product Gene Name   

anti-PKC-zeta antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 1; NC_000001.11 (2050470..2185395). Location: 1p36.33-p36.2
 OMIM    176982
 3D Structure    ModBase 3D Structure for Q05513
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 Clonality    Monoclonal
 Clone Number    R14-K
 Host    Rabbit
 Species Reactivity    Human, mouse, rat
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 Specificity    Human, rat, mouse-tested
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 Immunogen    Peptide derived from the human PKC-zeta sequence
 Storage Buffer    20 mM Tris-HCI, pH 8.0
 Stabilizer    10 mg/ml BSA
 Preservative    0.05% NaN3
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 Preparation and Storage    Store at -20 degree C
 ISO Certification    Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
 Other Notes    Small volumes of anti-PKC-zeta antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-PKC-zeta antibody   

ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

 Application Notes for anti-PKC-zeta antibody    WB - 1:5 00, ELISA - 1:20 000 - 1:100 000, ICC - 1:100 - 1:400
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 Testing Data of anti-PKC-zeta antibody    anti-PKC-zeta antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for PKC-zeta. It may not necessarily be applicable to this product.
 NCBI GI #    68067736
 NCBI GeneID    5590
 NCBI Accession #    Q05513.4 [Other Products]
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 UniProt Primary Accession #    Q05513 [Other Products]
 UniProt Secondary Accession #    Q15207; Q5SYT5; Q969S4; A8K4N0; A8MU64; B7Z2J7; E9PCW2 [Other Products]
 UniProt Related Accession #    Q05513 [Other Products]
 Molecular Weight    67,660 Da
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 NCBI Official Full Name    Protein kinase C zeta type
 NCBI Official Synonym Full Names    protein kinase C, zeta
 NCBI Official Symbol    PRKCZ [Similar Products]
 NCBI Official Synonym Symbols   
PKC2; PKC-ZETA
[Similar Products]
 NCBI Protein Information    protein kinase C zeta type; nPKC-zeta
 UniProt Protein Name    Protein kinase C zeta type
 UniProt Synonym Protein Names   
nPKC-zeta
 UniProt Gene Name    PRKCZ [Similar Products]
 UniProt Synonym Gene Names    PKC2 [Similar Products]
 UniProt Entry Name    KPCZ_HUMAN
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 NCBI Summary for PKC-zeta    Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
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 UniProt Comments for PKC-zeta    Function: Calcium- and diacylglycerol-independent serine/threonine-protein kinase that functions in phosphatidylinositol 3-kinase (PI3K) pathway and mitogen-activated protein (MAP) kinase cascade, and is involved in NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response and maintenance of long-term potentiation (LTP). Upon lipopolysaccharide (LPS) treatment in macrophages, or following mitogenic stimuli, functions downstream of PI3K to activate MAP2K1/MEK1-MAPK1/ERK2 signaling cascade independently of RAF1 activation. Required for insulin-dependent activation of AKT3, but may function as an adapter rather than a direct activator. Upon insulin treatment may act as a downstream effector of PI3K and contribute to the activation of translocation of the glucose transporter SLC2A4/GLUT4 and subsequent glucose transport in adipocytes. In EGF-induced cells, binds and activates MAP2K5/MEK5-MAPK7/ERK5 independently of its kinase activity and can activate JUN promoter through MEF2C. Through binding with SQSTM1/p62, functions in interleukin-1 signaling and activation of NF-kappa-B with the specific adapters RIPK1 and TRAF6. Participates in TNF-dependent transactivation of NF-kappa-B by phosphorylating and activating IKBKB kinase, which in turn leads to the degradation of NF-kappa-B inhibitors. In migrating astrocytes, forms a cytoplasmic complex with PARD6A and is recruited by CDC42 to function in the establishment of cell polarity along with the microtubule motor and dynein. In association with FEZ1, stimulates neuronal differentiation in PC12 cells. In inflammatory response, is required for the T-helper 2 (Th2) differentiation process, including interleukins production, efficient activation of JAK1 and the subsequent phosphorylation and nuclear translocation of STAT6. May be involved in development of allergic airway inflammation (asthma), a process dependent on Th2 immune response. In NF-kappa-B-mediated inflammatory response, can relieve the SETD6-dependent repression of NF-kappa-B target genes by phosphorylating the RELA subunit at 'Ser-311'. Is necessary and sufficient for LTP maintenance in hippocampal CA1 pyramidal cells. Ref.7 Ref.11 Ref.14 Ref.19 Ref.20

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation: Atypical PKCs (PRKCI and PRKCZ) exhibit an elevated basal enzymatic activity (that may be due to the interaction with SMG1 or SQSTM1) and are not regulated by diacylglycerol, phosphatidylserine, phorbol esters or calcium ions. Two specific sites, Thr-410 (activation loop of the kinase domain) and Thr-560 (turn motif), need to be phosphorylated for its full activation. Phosphatidylinositol 3,4,5-trisphosphate might be a physiological activator

By similarity.

Subunit structure: Forms a ternary complex with SQSTM1 and KCNAB2. Forms another ternary complex with SQSTM1 and GABRR3. Forms a complex with SQSTM1 and MAP2K5

By similarity. Interacts with PARD6A, PARD6B, PARD6G and SQSTM1. Part of a complex with PARD3, PARD6A or PARD6B or PARD6G and CDC42 or RAC1. Interacts with ADAP1/CENTA1. Forms a ternary complex composed of SQSTM1 and PAWR. Interacts directly with SQSTM1

Probable. Interacts with IKBKB. Interacts (via the protein kinase domain) with WWC1. Forms a tripartite complex with WWC1 and DDR1, but predominantly in the absence of collagen. Component of the Par polarity complex, composed of at least phosphorylated PRKCZ, PARD3 and TIAM1. Interacts with PDPK1 (via N-terminus region). Ref.8 Ref.10 Ref.12 Ref.13 Ref.15 Ref.16 Ref.17 Ref.18 Ref.22 Ref.25

Subcellular location: Cytoplasm. Endosome. Cell junction. Note: In the retina, localizes in the terminals of the rod bipolar cells

By similarity. Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction. Ref.8 Ref.25

Tissue specificity: Expressed in brain, and to a lesser extent in lung, kidney and testis.

Domain: The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B. Ref.18The C1 domain does not bind the diacylglycerol (DAG). Ref.18

Post-translational modification: CDH5 is required for its phosphorylation at Thr-410. Phosphorylated by protein kinase PDPK1; phosphorylation is inhibited by the apoptotic C-terminus cleavage product of PKN2. Phosphorylation at Thr-410 by PI3K activates the kinase. Ref.9 Ref.13 Ref.21 Ref.25

Sequence similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.Contains 1 AGC-kinase C-terminal domain.Contains 1 OPR domain.Contains 1 phorbol-ester/DAG-type zinc finger.Contains 1 protein kinase domain.

Sequence caution: The sequence CAA78813.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
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 Research Articles on PKC-zeta    1. Data indicate that both tumor focality and Par3/Par6/atypical protein kinase C (APKC) expression were significantly associated with tumor recurrence.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-PKC-zeta antibodyPathways associated with anti-PKC-zeta antibody
 Reference Product  PubMed Publications
 PARD3 antibody  >2 publications with PKC-zeta and PARD3
 AKT1 antibody  >2 publications with PKC-zeta and AKT1
 PARD6A antibody  >2 publications with PKC-zeta and PARD6A
 CDC42 antibody  >1 publications with PKC-zeta and CDC42
 PDPK1 antibody  >1 publications with PKC-zeta and PDPK1
 RELA antibody  >1 publications with PKC-zeta and RELA
 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 CDC42 Signaling Events Pathway antibodies  CDC42 Signaling Events Pathway Diagram
 CXCR4-mediated Signaling Events Pathway antibodies  CXCR4-mediated Signaling Events Pathway Diagram
 Calcium Regulation In The Cardiac Cell Pathway antibodies  Calcium Regulation In The Cardiac Cell Pathway Diagram
 Ceramide Signaling Pathway antibodies  Ceramide Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
Diseases associated with anti-PKC-zeta antibodyOrgans/Tissues associated with anti-PKC-zeta antibody
 Disease Name  Pubmed Publications
 Heart Diseases Antibodies  >4 publications with PKC-zeta and Heart Diseases
 Cardiovascular Diseases Antibodies  >3 publications with PKC-zeta and Cardiovascular Diseases
 Nervous System Diseases Antibodies  >2 publications with PKC-zeta and Nervous System Diseases
 Hypertrophy Antibodies  >2 publications with PKC-zeta and Hypertrophy
 Leukemia Antibodies  >2 publications with PKC-zeta and Leukemia
 Prostatic Neoplasms Antibodies  >2 publications with PKC-zeta and Prostatic Neoplasms
 Kidney Diseases Antibodies  >1 publications with PKC-zeta and Kidney Diseases
 Edema Antibodies  >1 publications with PKC-zeta and Edema
 Fatty Liver Antibodies  >1 publications with PKC-zeta and Fatty Liver
 Inflammation Antibodies  >1 publications with PKC-zeta and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >10 publications with PKC-zeta and Liver
 Heart Antibodies  >7 publications with PKC-zeta and Heart
 Brain Antibodies  >7 publications with PKC-zeta and Brain
 Kidney Antibodies  >4 publications with PKC-zeta and Kidney
 Lung Antibodies  >3 publications with PKC-zeta and Lung
 Eye Antibodies  >3 publications with PKC-zeta and Eye
 Prostate Antibodies  >2 publications with PKC-zeta and Prostate
 Uterus Antibodies  >2 publications with PKC-zeta and Uterus
 Ovary Antibodies  >1 publications with PKC-zeta and Ovary
 Lymph Antibodies  >1 publications with PKC-zeta and Lymph
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