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anti-PTCH1 antibody :: Mouse anti-Human PTCH1 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS834406
Unit / Price
0.1 mL  /  $515 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PTCH1, Monoclonal Antibody

 Also Known As   

PTCH1 antibody

 Product Synonym Names    Monoclonal PTCH1; Anti-PTCH1; Patched 1; PTCH1; PTCH 1; PTCH-1
 Product Gene Name   

anti-PTCH1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    109400
 3D Structure    ModBase 3D Structure for Q13635
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 Clonality    Monoclonal
 Isotype    IgG2b
 Clone Number    3c2
 Host    Mouse
 Species Reactivity    Human
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 Purity/Purification    PTCH1 antibody was purified by affinity chromatography.
 Form/Format    Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
 Concentration    500 ug-1 mg/ml (lot specific)
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 Biological Significance    Mutations of the human Patched tumor suppressor gene (PTCH) have been identified in individuals with the nevoid basal cell carcinoma syndrome (NBCCS) as well as in sporadic basal cell carcinomas and medulloblastomas.
 Immunogen    PTCH1 antibody was raised in mouse using a recombinant protein expressed in E Coli corresponding to amino acids 122-436 of human Patched1 as the immunogen.
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 Preparation and Storage    Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
 Other Notes    Small volumes of anti-PTCH1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-PTCH1 antibody

   Mouse monoclonal PTCH1 antibody
 Product Categories/Family for anti-PTCH1 antibody    Cancer
 Applications Tested/Suitable for anti-PTCH1 antibody   

Immunofluorescence (IF), Western Blot (WB)

 Application Notes for anti-PTCH1 antibody    IF: 1:100
WB: 1:500
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 Immunofluorescence (IF) of anti-PTCH1 antibody    Immunofluorescent staining of Hela cells transiently transfected with recombinant PTCH1 protein using PTCH1 antibody
anti-PTCH1 antibody Immunofluorescence (IF) image
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 Western Blot (WB) of anti-PTCH1 antibody    Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PTCH1 protein (Right) or empty vector (Left) detected with PTCH1 antibody
anti-PTCH1 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for PTCH1. It may not necessarily be applicable to this product.
 NCBI GI #    134254468
 NCBI GeneID    5727
 NCBI Accession #    NP_001077074.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001083605.1 [Other Products]
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 UniProt Primary Accession #    Q13635 [Other Products]
 UniProt Secondary Accession #    Q13463; Q5R1U7; Q5R1U9; Q5R1V0; Q5VZC0; Q5VZC2; Q86XG7; A3KBI9; E9PEJ8 [Other Products]
 UniProt Related Accession #    Q13635 [Other Products]
 Molecular Weight    144,356 Da
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 NCBI Official Full Name    protein patched homolog 1 isoform S
 NCBI Official Synonym Full Names    patched 1
 NCBI Official Symbol    PTCH1 [Similar Products]
 NCBI Official Synonym Symbols   
PTC; BCNS; HPE7; PTC1; PTCH; NBCCS; PTCH11
[Similar Products]
 NCBI Protein Information    protein patched homolog 1
 UniProt Protein Name    Protein patched homolog 1
 UniProt Gene Name    PTCH1 [Similar Products]
 UniProt Synonym Gene Names    PTCH; PTC; PTC1 [Similar Products]
 UniProt Entry Name    PTC1_HUMAN
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 NCBI Summary for PTCH1    This gene encodes a member of the patched gene family. The encoded protein is the receptor for sonic hedgehog, a secreted molecule implicated in the formation of embryonic structures and in tumorigenesis, as well as the desert hedgehog and indian hedgehog proteins. This gene functions as a tumor suppressor. Mutations of this gene have been associated with basal cell nevus syndrome, esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, as well as holoprosencephaly. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences and biological validity cannot be determined currently. [provided by RefSeq, Jul 2008]
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 UniProt Comments for PTCH1    PTCH1: a multi-pass membrane protein member of the ?patched? family that acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog protein?s signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. Interacts with SNX17. Expressed in tumor cells but not in normal skin. In the embryo, found in all major target tissues of sonic hedgehog, such as the ventral neural tube, somites, and tissues surrounding the zone of polarizing activity of the limb bud. Defects in PTCH1 are the cause of basal cell nevus syndrome (BCNS), also known as Gorlin syndrome. BCNS is an autosomal dominant disease characterized by nevoid basal cell carcinomas and developmental abnormalities such as rib and craniofacial alterations, polydactyly, syndactyly, and spina bifida. In addition, the patients suffer from a multitude of tumors like basal cell carcinomas, fibromas of the ovaries and heart, cysts of the skin, jaws and mesentery, as well as medulloblastomas and meningiomas. PTCH1 defects is also the cause of holoprosencephaly, the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres.

Protein type: Tumor suppressor; Cell cycle regulation; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 9q22.3

Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; perinuclear region of cytoplasm; postsynaptic density; integral to membrane; plasma membrane; midbody; caveola

Molecular Function: heparin binding; cyclin binding; protein binding; hedgehog receptor activity; cholesterol binding; protein complex binding; smoothened binding; patched binding

Biological Process: heart morphogenesis; hindlimb morphogenesis; epidermis development; regulation of mitotic cell cycle; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; response to chlorate; response to estradiol stimulus; regulation of protein localization; negative regulation of osteoblast differentiation; embryonic limb morphogenesis; negative regulation of epithelial cell proliferation; response to drug; smoothened signaling pathway; pharyngeal system development; response to retinoic acid; negative regulation of multicellular organism growth; in utero embryonic development; negative regulation of transcription factor activity; neural tube patterning; negative regulation of cell division; keratinocyte proliferation; spinal cord motor neuron differentiation; limb morphogenesis; organ morphogenesis; dorsal/ventral pattern formation; ureteric bud branching; response to mechanical stimulus; negative regulation of smoothened signaling pathway; neural plate pattern formation; neural tube closure; embryonic organ development; protein processing; brain development; regulation of smoothened signaling pathway

Disease: Holoprosencephaly 7; Basal Cell Nevus Syndrome; Basal Cell Carcinoma, Susceptibility To, 1
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 Research Articles on PTCH1    1. our data suggests that activation of the Hedgehog pathway due to PTCH1 inactivation along with HPV infection is important in cervical cancer development.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-PTCH1 antibodyDiseases associated with anti-PTCH1 antibody
 Products by Pathway  Pathway Diagram
 Activation Of SMO Pathway antibodies  Activation Of SMO Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Class B/2 (Secretin Family Receptors) Pathway antibodies  Class B/2 (Secretin Family Receptors) Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Glypican 3 Network Pathway antibodies  Glypican 3 Network Pathway Diagram
 Hedgehog 'off' State Pathway antibodies  Hedgehog 'off' State Pathway Diagram
 Hedgehog 'on' State Pathway antibodies  Hedgehog 'on' State Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Disease Name  Pubmed Publications
 Carcinoma, Basal Cell Antibodies  >183 publications with PTCH1 and Carcinoma, Basal Cell
 Medulloblastoma Antibodies  >90 publications with PTCH1 and Medulloblastoma
 Basal Cell Nevus Syndrome Antibodies  >90 publications with PTCH1 and Basal Cell Nevus Syndrome
 Skin Neoplasms Antibodies  >85 publications with PTCH1 and Skin Neoplasms
 Neuroectodermal Tumors, Primitive Antibodies  >53 publications with PTCH1 and Neuroectodermal Tumors, Primitive
 Brain Neoplasms Antibodies  >47 publications with PTCH1 and Brain Neoplasms
 Disease Models, Animal Antibodies  >44 publications with PTCH1 and Disease Models, Animal
 Cell Transformation, Neoplastic Antibodies  >24 publications with PTCH1 and Cell Transformation, Neoplastic
 Neoplasms, Experimental Antibodies  >24 publications with PTCH1 and Neoplasms, Experimental
 Rhabdomyosarcoma Antibodies  >23 publications with PTCH1 and Rhabdomyosarcoma
Organs/Tissues associated with anti-PTCH1 antibody
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >107 publications with PTCH1 and Skin
 Brain Antibodies  >90 publications with PTCH1 and Brain
 Embryonic Tissue Antibodies  >32 publications with PTCH1 and Embryonic Tissue
 Lung Antibodies  >25 publications with PTCH1 and Lung
 Muscle Antibodies  >23 publications with PTCH1 and Muscle
 Stomach Antibodies  >15 publications with PTCH1 and Stomach
 Mouth Antibodies  >15 publications with PTCH1 and Mouth
 Eye Antibodies  >14 publications with PTCH1 and Eye
 Connective Tissue Antibodies  >13 publications with PTCH1 and Connective Tissue
 Prostate Antibodies  >12 publications with PTCH1 and Prostate
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