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anti-BBC3 antibody :: Mouse anti-Human, Rat PUMA Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS150004
Western Blot (WB)
Unit / Price
0.1 mg  /  $365 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PUMA (BBC3), Monoclonal Antibody

 Also Known As   

PUMA Antibody [10D4G7]

 Product Synonym Names    PUMA Antibody [10D4G7] : JFY1; PUMA; JFY-1; BCL2 binding component 3
 Product Gene Name   

anti-BBC3 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    605854
 3D Structure    ModBase 3D Structure for Q9BXH1
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 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    10D4G7
 Host    Mouse
 Species Reactivity    Human, Rat
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 Purity/Purification    PUMA Monoclonal Antibody is immunoaffinity chromotography purified IgG.
 Form/Format    Liquid
 Concentration    1 mg/mL (lot specific)
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 Conjugate    Unconjugated
 Immunogen    Recombinant protein corresponding to amino acids 76 - 170 of human PUMA-alpha.
 Buffer    PUMA Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
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 Preparation and Storage    PUMA monoclonal antibody can be stored at -20 degree C, stable for one year.
 Other Notes    Small volumes of anti-BBC3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-BBC3 antibody

   PUMA Monoclonal Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMAα and PUMAβ. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
 Applications Tested/Suitable for anti-BBC3 antibody   

ELISA (EIA), Western Blot (WB)

 Application Notes for anti-BBC3 antibody    PUMA antibody can be used for detection of PUMA by Western blot at 2.5 to 5 mug/mL.
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 Western Blot (WB) of anti-BBC3 antibody    Western blot analysis of PUMA expression in K562 cell lysate with PUMA antibody at (A) 2.5 and (B) 5 μg/mL.
anti-BBC3 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for BBC3. It may not necessarily be applicable to this product.
 NCBI GI #    56748610
 NCBI GeneID    27113
 NCBI Accession #    Q9BXH1.1 [Other Products]
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 UniProt Primary Accession #    Q9BXH1 [Other Products]
 UniProt Secondary Accession #    O00171; Q96PG9; B9EGI3 [Other Products]
 UniProt Related Accession #    Q96PG8; Q9BXH1 [Other Products]
 Molecular Weight    10,326 Da
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 NCBI Official Full Name    Bcl-2-binding component 3
 NCBI Official Synonym Full Names    BCL2 binding component 3
 NCBI Official Symbol    BBC3 [Similar Products]
 NCBI Official Synonym Symbols   
JFY1; PUMA; JFY-1
[Similar Products]
 NCBI Protein Information    bcl-2-binding component 3; p53 up-regulated modulator of apoptosis
 UniProt Protein Name    Bcl-2-binding component 3
 UniProt Synonym Protein Names   
JFY-1; p53 up-regulated modulator of apoptosis
 Protein Family    Bcl-2-binding component
 UniProt Gene Name    BBC3 [Similar Products]
 UniProt Synonym Gene Names    PUMA [Similar Products]
 UniProt Entry Name    BBC3_HUMAN
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 NCBI Summary for BBC3    This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]
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 UniProt Comments for BBC3    PUMA: Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Interacts with MCL1 and BCL2A1. Interacts with BCL2 and BCL2L1/BCL-XL. By DNA damage, glucocorticoid treatment, growth factor deprivation and p53/TP53. Ubiquitously expressed. Belongs to the Bcl-2 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: mitochondrial outer membrane; mitochondrion; cytosol

Molecular Function: protein binding

Biological Process: caspase activation; release of cytochrome c from mitochondria; positive regulation of protein homooligomerization; apoptosis; release of sequestered calcium ion into cytosol; positive regulation of neuron apoptosis; reduction of endoplasmic reticulum calcium ion concentration; determination of adult life span; response to DNA damage stimulus; negative regulation of growth
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 Research Articles on BBC3    1. c-Jun transactivates Puma gene expression to promote osteoarthritis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-BBC3 antibodyPathways associated with anti-BBC3 antibody
 Reference Product  PubMed Publications
 PMAIP1 antibody  >72 publications with BBC3 and PMAIP1
 TP53 antibody  >59 publications with BBC3 and TP53
 BCL2L1 antibody  >46 publications with BBC3 and BCL2L1
 BID antibody  >26 publications with BBC3 and BID
 BAD antibody  >24 publications with BBC3 and BAD
 MCL1 antibody  >17 publications with BBC3 and MCL1
 SP1 antibody  >3 publications with BBC3 and SP1
 SNAI2 antibody  >3 publications with BBC3 and SNAI2
 BCL2L2 antibody  >3 publications with BBC3 and BCL2L2
 Products by Pathway  Pathway Diagram
 Activation Of BH3-only Proteins Pathway antibodies  Activation Of BH3-only Proteins Pathway Diagram
 Activation Of PUMA And Translocation To Mitochondria Pathway antibodies  Activation Of PUMA And Translocation To Mitochondria Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Modulation And Signaling Pathway antibodies  Apoptosis Modulation And Signaling Pathway Diagram
 BH3-only Proteins Associate With And Inactivate Anti-apoptotic BCL-2 Members Pathway antibodies  BH3-only Proteins Associate With And Inactivate Anti-apoptotic BCL-2 Members Pathway Diagram
 DNA Damage Response Pathway antibodies  DNA Damage Response Pathway Diagram
 DNA Damage Response (only ATM Dependent) Pathway antibodies  DNA Damage Response (only ATM Dependent) Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
Diseases associated with anti-BBC3 antibodyOrgans/Tissues associated with anti-BBC3 antibody
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >73 publications with BBC3 and Carcinoma
 Adenocarcinoma Antibodies  >33 publications with BBC3 and Adenocarcinoma
 Breast Neoplasms Antibodies  >32 publications with BBC3 and Breast Neoplasms
 Lung Neoplasms Antibodies  >24 publications with BBC3 and Lung Neoplasms
 Necrosis Antibodies  >20 publications with BBC3 and Necrosis
 Liver Diseases Antibodies  >18 publications with BBC3 and Liver Diseases
 Lung Diseases Antibodies  >17 publications with BBC3 and Lung Diseases
 Nervous System Diseases Antibodies  >16 publications with BBC3 and Nervous System Diseases
 Carcinoma, Squamous Cell Antibodies  >13 publications with BBC3 and Carcinoma, Squamous Cell
 Brain Diseases Antibodies  >13 publications with BBC3 and Brain Diseases
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >30 publications with BBC3 and Lung
 Brain Antibodies  >18 publications with BBC3 and Brain
 Kidney Antibodies  >13 publications with BBC3 and Kidney
 Skin Antibodies  >12 publications with BBC3 and Skin
 Prostate Antibodies  >12 publications with BBC3 and Prostate
 Stomach Antibodies  >12 publications with BBC3 and Stomach
 Muscle Antibodies  >9 publications with BBC3 and Muscle
 Bone Antibodies  >9 publications with BBC3 and Bone
 Eye Antibodies  >7 publications with BBC3 and Eye
 Intestine Antibodies  >6 publications with BBC3 and Intestine
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