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anti-PKBa antibody :: Rabbit anti-Mouse Phosphorylated Protein Kinase B Alpha (PKBa) Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS2090673 anti-PKBa antibody
Unit / Price
0.02 mg  /  $115 +1 FREE 8GB USB
0.1 mg  /  $195 +1 FREE 8GB USB
0.2 mg  /  $255 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Phosphorylated Protein Kinase B Alpha (PKBa), Monoclonal Antibody

 Also Known As   

Anti-Phosphorylated Protein Kinase B Alpha (PKBa) Monoclonal Antibody

 Product Synonym Names    AKT1; AKT; PKB-A; Proto-oncogene c-Akt; RAC-Alpha Serine/Threonine-Protein Kinase; V-akt Murine Thymoma Viral Oncogene Homolog 1
 Product Gene Name   

anti-PKBa antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 3D Structure    ModBase 3D Structure for P31750
 Clonality    Monoclonal
 Isotype    IgG
 Clone Number    D4
 Host    Rabbit
 Species Reactivity    Mouse
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 Specificity    The antibody is a mouse monoclonal antibody raised against PKBa. It has been selected for its ability to recognize PKBa in immunohistochemical staining and western blotting.
 Purity/Purification    Protein A + Protein G affinity chromatography
 Form/Format    Liquid
 Concentration    1mg/mL (lot specific)
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 Immunogen    Phosphorylated Protein Kinase B Alpha (PKBa)
 Buffer Formulation    0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
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 Preparation and Storage    Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Other Notes    Small volumes of anti-PKBa antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-PKBa antibody   

Western Blot (WB)

 Application Notes for anti-PKBa antibody    Immunocytochemistry in formalin fixed cells: 5-30ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml
Immunohistochemistry in paraffin section: 5-30ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
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NCBI/Uniprot data below describe general gene information for PKBa. It may not necessarily be applicable to this product.
 NCBI GI #    260166608
 NCBI GeneID    11651
 NCBI Accession #    NP_001159366.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001165894.1 [Other Products]
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 UniProt Primary Accession #    P31750 [Other Products]
 UniProt Secondary Accession #    Q62274; Q6GSA6 [Other Products]
 UniProt Related Accession #    P31750 [Other Products]
 Molecular Weight    55,707 Da
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 NCBI Official Full Name    RAC-alpha serine/threonine-protein kinase isoform 2
 NCBI Official Synonym Full Names    thymoma viral proto-oncogene 1
 NCBI Official Symbol    Akt1 [Similar Products]
 NCBI Official Synonym Symbols   
Akt; PKB; Rac; LTR-akt; PKB/Akt; PKBalpha
[Similar Products]
 NCBI Protein Information    RAC-alpha serine/threonine-protein kinase
 UniProt Protein Name    RAC-alpha serine/threonine-protein kinase
 UniProt Synonym Protein Names   
AKT1 kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; Thymoma viral proto-oncogene
 Protein Family    RAC family serine/threonine-protein kinase
 UniProt Gene Name    Akt1 [Similar Products]
 UniProt Synonym Gene Names    Akt; Rac; PKB; PKB alpha [Similar Products]
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 NCBI Summary for PKBa    This gene encodes the founding member of the Akt serine-threonine protein kinase gene family that also includes Akt2 and Akt3. This kinase is a major downstream effector of the phosphatidylinositol 3-kinase (PI3K) pathway that mediates the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). It is activated through recruitment to cellular membranes by PI3K lipid products and by phosphorylation by 3-phosphoinositide dependent kinase-1. It then further phosphorylates different downstream proteins in response to various extracellular signals and thus plays a pivotal role in mediating a variety of cellular processes, such as glucose metabolism, glycogen biosynthesis, protein synthesis and turn over, inflammatory response, cell survival (anti-apoptosis) and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
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 UniProt Comments for PKBa    Akt1: an oncogenic AGC kinase that plays a critical role in regulating cell survival and metabolism in many different signaling pathways. Dual phosphorylation is required for its activation. T308 is phosphorylated by PDK1 in the PI3 kinase pathway, and S473 is phosphorylated by mTOR in the mTORC2 pathway. The 'Lys-63'-linked ubiquitination of AKT1 by TRAF6 is important for its translocation to the plasma membrane, phosphorylation, and activation. When Akt is fully phosphorylated it translocates into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its proteosomal degradation. Hyperactive or overexpressed in a number of cancers including breast, prostate, lung, pancreatic, liver, ovarian and colorectal. Over 160 protein substrates are known including many that regulate transcription, metabolism, apoptosis, cell cycle, and growth.

Protein type: AGC group; AKT family; EC 2.7.11.1; Kinase, protein; Oncoprotein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 12 F1|12 61.2 cM

Cellular Component: cytoplasm; cytosol; intercellular junction; membrane; microtubule cytoskeleton; mitochondrion; nucleus; plasma membrane; protein complex; spindle; vesicle

Molecular Function: ATP binding; enzyme binding; GTPase activating protein binding; identical protein binding; kinase activity; nitric-oxide synthase regulator activity; nucleotide binding; phosphatidylinositol-3,4,5-triphosphate binding; phosphatidylinositol-3,4-bisphosphate binding; protein binding; protein homodimerization activity; protein kinase activity; protein kinase binding; protein kinase C binding; protein phosphatase 2A binding; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity; transferase activity

Biological Process: activated T cell apoptosis; aging; anagen; apoptosis; apoptotic mitochondrial changes; carbohydrate metabolic process; carbohydrate transport; cell projection organization and biogenesis; cellular response to insulin stimulus; cytoskeleton organization and biogenesis; epidermal growth factor receptor signaling pathway; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; germ cell development; glucose homeostasis; glucose metabolic process; glucose transport; glycogen biosynthetic process; glycogen metabolic process; I-kappaB kinase/NF-kappaB cascade; inflammatory response; insulin receptor signaling pathway; insulin-like growth factor receptor signaling pathway; lipopolysaccharide-mediated signaling pathway; maternal placenta development; multicellular organismal development; myelin maintenance in the peripheral nervous system; negative regulation of apoptosis; negative regulation of autophagy; negative regulation of caspase activity; negative regulation of cell size; negative regulation of fatty acid beta-oxidation; negative regulation of JNK cascade; negative regulation of protein kinase activity; negative regulation of proteolysis; nervous system development; osteoblast differentiation; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; phosphoinositide 3-kinase cascade; phosphorylation; positive regulation of apoptosis; positive regulation of blood vessel endothelial cell migration; positive regulation of cell growth; positive regulation of cell proliferation; positive regulation of cellular protein metabolic process; positive regulation of endodeoxyribonuclease activity; positive regulation of endothelial cell proliferation; positive regulation of fat cell differentiation; positive regulation of glucose import; positive regulation of glycogen biosynthetic process; positive regulation of lipid biosynthetic process; positive regulation of nitric oxide biosynthetic process; positive regulation of nitric-oxide synthase activity; positive regulation of organ growth; positive regulation of peptidyl-serine phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein amino acid phosphorylation; positive regulation of smooth muscle cell proliferation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of vasoconstriction; protein amino acid phosphorylation; protein catabolic process; protein import into nucleus, translocation; protein kinase B signaling cascade; protein ubiquitination; regulation of apoptosis; regulation of cell migration; regulation of glycogen biosynthetic process; regulation of myelination; regulation of protein localization; regulation of translation; response to DNA damage stimulus; response to food; response to hormone stimulus; response to organic substance; signal transduction; spinal cord development; striated muscle cell differentiation; translation; transport
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 Research Articles on PKBa    1. Tideglusib significantly reduced cerebral infarct volume at both 24h and 7days after HI injury. Tideglusib also increased phosphorylated GSK-3beta(Ser9) and Akt(Ser473)
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-PKBa antibodyPathways associated with anti-PKBa antibody
 Reference Product  PubMed Publications
 Pten antibody  >635 publications with PKBa and Pten
 Mtor antibody  >576 publications with PKBa and Mtor
 Pik3ca antibody  >317 publications with PKBa and Pik3ca
 Foxo1 antibody  >167 publications with PKBa and Foxo1
 Foxo3 antibody  >79 publications with PKBa and Foxo3
 Tsc2 antibody  >47 publications with PKBa and Tsc2
 Pik3r1 antibody  >32 publications with PKBa and Pik3r1
 Rictor antibody  >31 publications with PKBa and Rictor
 Creb1 antibody  >14 publications with PKBa and Creb1
 Hsp90aa1 antibody  >5 publications with PKBa and Hsp90aa1
 Products by Pathway  Pathway Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 AKT Phosphorylates Targets In The Cytosol Pathway antibodies  AKT Phosphorylates Targets In The Cytosol Pathway Diagram
 AKT Phosphorylates Targets In The Nucleus Pathway antibodies  AKT Phosphorylates Targets In The Nucleus Pathway Diagram
 AKT-mediated Inactivation Of FOXO1A Pathway antibodies  AKT-mediated Inactivation Of FOXO1A Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
Diseases associated with anti-PKBa antibodyOrgans/Tissues associated with anti-PKBa antibody
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >512 publications with PKBa and Breast Neoplasms
 Disease Models, Animal Antibodies  >342 publications with PKBa and Disease Models, Animal
 Necrosis Antibodies  >332 publications with PKBa and Necrosis
 Lung Neoplasms Antibodies  >324 publications with PKBa and Lung Neoplasms
 Inflammation Antibodies  >244 publications with PKBa and Inflammation
 Neoplasms, Experimental Antibodies  >230 publications with PKBa and Neoplasms, Experimental
 Prostatic Neoplasms Antibodies  >222 publications with PKBa and Prostatic Neoplasms
 Liver Diseases Antibodies  >183 publications with PKBa and Liver Diseases
 Colorectal Neoplasms Antibodies  >183 publications with PKBa and Colorectal Neoplasms
 Heart Diseases Antibodies  >182 publications with PKBa and Heart Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >917 publications with PKBa and Blood
 Muscle Antibodies  >777 publications with PKBa and Muscle
 Brain Antibodies  >610 publications with PKBa and Brain
 Lung Antibodies  >491 publications with PKBa and Lung
 Liver Antibodies  >372 publications with PKBa and Liver
 Heart Antibodies  >358 publications with PKBa and Heart
 Bone Antibodies  >220 publications with PKBa and Bone
 Kidney Antibodies  >189 publications with PKBa and Kidney
 Skin Antibodies  >183 publications with PKBa and Skin
 Embryonic Tissue Antibodies  >145 publications with PKBa and Embryonic Tissue
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