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Mouse Phosphotyrosine Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS673080 anti-Phosphotyrosine antibody
Unit / Price
1 mL  /  $320 +1 FREE 8GB USB
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 Product Name   

Phosphotyrosine, Monoclonal Antibody

 Also Known As   

Mouse Anti-Phosphotyrosine-AP

 Product Synonym Names    Mouse Anti-Phosphotyrosine- Alkaline Phosphatase-Labeled; Mouse Anti-Phosphotyrosine
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Monoclonal
 Isotype    Mouse IgG2b kappa
 Clone Number    PY20
 Host    Mouse
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 Specificity    Phosphorylated tyrosine and phenylphosphate
Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g. via growth factors) during normal growth and development, and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation are cell surface glycoproteins. Antibody to phosphotyrosine enables the detection, isolation and characterization of proteins containing phosphotyrosine. PY20 also prevents internalization of activated receptors, e.g. EGFr, when microinjected into cells. The affinity of PY20 for phosphotyrosine is approximately 10^-6 to 10^-7 M. PY20 binding to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by phosphoserine, phosphothreonine or free phosphate. ^1-3
 Form/Format    Alkaline (AP) Phosphatase Conjugate
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 Characterization    To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested to conform to characteristics of a standard reference reagent by ELISA and/or Fluorescent ELSA. Representative data are included in this technical bulletin.
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 Preparation and Storage    The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C.
The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Reagents are stable for the period shown on the label if stored as directed.
 Other Notes    Small volumes of anti-Phosphotyrosine antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-Phosphotyrosine antibody   

ELISA; Immunofluorescence; Western Blot

 Application Notes for anti-Phosphotyrosine antibody    Research Application(s): Detection of phosporylated tyrosine-containing proteins on Western blots
Immunoaffinity purification of tyrosine-phosporylated proteins
Quantitation of phosphotyrosine by Enzyme-Linked-Immunosorbent-Assay (ELISA)

ELISA Suggested Dilution: 1:500-1:1,000
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Product References and Citations for anti-Phosphotyrosine antibody

   1. Glenney, J. R., Jr., L. Zokas, and M. P. Kamps. 1988. J. Immunol. Methods 109:277.
2. Ruff-Jamison, S., R. Campos-Gonzalez, and J. R. Glenney, Jr. 1991. J. Biol. Chem. 266:6607.
3. Glenney, J. R., Jr., W. S. Chen, C. S. Lazar, G. M. Walton, L. M. Zokas, M. G. Rosenfeld, and G. N. Gill. 1988. Cell 52:675.
4. Takagi, S., M. Daibata, T. J. Last, R. E. Humphreys, D. C. Parker, and T. Sairenji. 1991. J. Exp. Med 174:381.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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