Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Antibody rightarrow Phosphotyrosine rightarrow Monoclonal Phosphotyrosine  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Putrescine aminotransferase (patA) Recombinant Protein
 • lysozyme G-like 1 (LYG1) ELISA Kit
 • ACAA2 siRNA
 • CDKN2A ELISA Kit
 • Myelin Basic Protein Autoantibody (MBP) ELISA Kit
 • 6-phosphofructokinase, liver type (PFKL), partial Recombinant Protein
 • E. coli Antibody
 • ABHD4 Antibody
 • TOM1 cDNA Clone
 • Pepsinogen I ELISA Kit
 • Alanine--tRNA ligase (alaS), partial Recombinant Protein
 • Uncharacterized ABC transporter ATP-binding protein YvrO (yv... Recombinant Protein
 • Fibroblast Growth Factor 8, Androgen Induced (FGF8) ELISA Kit
 • Insulin Like Protein 6 (INSL6) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

Mouse Phosphotyrosine Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS673080 anti-Phosphotyrosine antibody
Unit / Price
1 mL  /  $320 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Phosphotyrosine, Monoclonal Antibody

 Also Known As   

Mouse Anti-Phosphotyrosine-AP

 Product Synonym Names    Mouse Anti-Phosphotyrosine- Alkaline Phosphatase-Labeled; Mouse Anti-Phosphotyrosine
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 Clonality    Monoclonal
 Isotype    Mouse IgG2b kappa
 Clone Number    PY20
 Host    Mouse
Section Bar
 Specificity    Phosphorylated tyrosine and phenylphosphate
Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g. via growth factors) during normal growth and development, and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation are cell surface glycoproteins. Antibody to phosphotyrosine enables the detection, isolation and characterization of proteins containing phosphotyrosine. PY20 also prevents internalization of activated receptors, e.g. EGFr, when microinjected into cells. The affinity of PY20 for phosphotyrosine is approximately 10^-6 to 10^-7 M. PY20 binding to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by phosphoserine, phosphothreonine or free phosphate. ^1-3
 Form/Format    Alkaline (AP) Phosphatase Conjugate
Section Bar
 Characterization    To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested to conform to characteristics of a standard reference reagent by ELISA and/or Fluorescent ELSA. Representative data are included in this technical bulletin.
Section Bar
 Preparation and Storage    The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C.
The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Reagents are stable for the period shown on the label if stored as directed.
 Other Notes    Small volumes of anti-Phosphotyrosine antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 Applications Tested/Suitable for anti-Phosphotyrosine antibody   

ELISA; Immunofluorescence; Western Blot

 Application Notes for anti-Phosphotyrosine antibody    Research Application(s): Detection of phosporylated tyrosine-containing proteins on Western blots
Immunoaffinity purification of tyrosine-phosporylated proteins
Quantitation of phosphotyrosine by Enzyme-Linked-Immunosorbent-Assay (ELISA)

ELISA Suggested Dilution: 1:500-1:1,000
Table BarTOPTable Bar
 

Product References and Citations for anti-Phosphotyrosine antibody

   1. Glenney, J. R., Jr., L. Zokas, and M. P. Kamps. 1988. J. Immunol. Methods 109:277.
2. Ruff-Jamison, S., R. Campos-Gonzalez, and J. R. Glenney, Jr. 1991. J. Biol. Chem. 266:6607.
3. Glenney, J. R., Jr., W. S. Chen, C. S. Lazar, G. M. Walton, L. M. Zokas, M. G. Rosenfeld, and G. N. Gill. 1988. Cell 52:675.
4. Takagi, S., M. Daibata, T. J. Last, R. E. Humphreys, D. C. Parker, and T. Sairenji. 1991. J. Exp. Med 174:381.
Section Bar
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions