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anti-Pin1 antibody :: Mouse anti-Human Pin1 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS200035
Western Blot (WB)
Unit / Price
0.05 mL  /  $240 +1 FREE 8GB USB
0.1 mL  /  $315 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Pin1, Monoclonal Antibody

 Also Known As   

Pin1 antibody

 Product Synonym Names    Protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1; PIN1; Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; EC 5.2.1.8; Rotamase Pin1; PPIase Pin1; DOD; uBL5; PIN1; PPIase; EC 5.2.1.8; Rotamase Pin1; PPIase Pin1; Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1
 Product Gene Name   

anti-Pin1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    U49070 mRNA
 3D Structure    ModBase 3D Structure for Q13526
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 Clonality    Monoclonal
 Isotype    IgG1, k
 Clone Number    3G8
 Host    Mouse
 Species Reactivity    Human
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 Purity/Purification    By protein-G affinity chromatography
 Form/Format    Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
 Concentration    1mg/ml (lot specific)
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 Antigen Species    Human
 Immunogen    Recombinant human Pin1 (1-163aa) purified from E. coli
 Clone Note    Anti-human Pin1 mAb, clone 3G8, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Pin1 protein.
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 Preparation and Storage    Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of anti-Pin1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-Pin1 antibody

   Human Pin 1 is a peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and essential for cell cycle regulation. Pin1 is nuclear PPIase containing a WW protein interaction domain, and is structurally and functionally related to Ess1/Ptf1, an essential protein in budding yeast. PPIase activity is necessary for Ess1/Pin1 function in yeast. Pin1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Substrates of Pin1 include the mitotic regulators (Cdc25 phosphatase and NIMA,PLk I, Wee, and Myt1 kinases), several transcription factors likebeta-Catenin, c-Jun, and the tumor suppressor protein p53, and some specific proteins like the RNA Pol II, the cytoskeleton protein tau, and the G1/S protein Cyclin D1.
 Product Categories/Family for anti-Pin1 antibody    Neuroscience
 Applications Tested/Suitable for anti-Pin1 antibody   

ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS)

 Application Notes for anti-Pin1 antibody    WB: 1:500 ~ 1,000.
Recommended starting dilution is 1:500.
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 Western Blot (WB) of anti-Pin1 antibody    Cell lysates of 293T, HeLa and Jurkat (each 50ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Pin1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
anti-Pin1 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for Pin1. It may not necessarily be applicable to this product.
 NCBI GI #    5453898
 NCBI GeneID    5300
 NCBI Accession #    NP_006212 [Other Products]
 NCBI GenBank Nucleotide #    NM_006221.3 [Other Products]
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 UniProt Primary Accession #    Q13526 [Other Products]
 UniProt Secondary Accession #    Q53X75; A8K4V9 [Other Products]
 UniProt Related Accession #    Q13526 [Other Products]
 Molecular Weight    18,243 Da
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 NCBI Official Full Name    peptidyl-prolyl cis-trans isomerase NIMA-interacting 1
 NCBI Official Synonym Full Names    peptidylprolyl cis/trans isomerase, NIMA-interacting 1
 NCBI Official Symbol    PIN1 [Similar Products]
 NCBI Official Synonym Symbols   
DOD; UBL5
[Similar Products]
 NCBI Protein Information    peptidyl-prolyl cis-trans isomerase NIMA-interacting 1
 UniProt Protein Name    Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1
 UniProt Synonym Protein Names   
Peptidyl-prolyl cis-trans isomerase Pin1; PPIase Pin1; Rotamase Pin1
 Protein Family    Peptidyl-prolyl cis-trans isomerase
 UniProt Gene Name    PIN1 [Similar Products]
 UniProt Synonym Gene Names    PPIase Pin1 [Similar Products]
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 NCBI Summary for Pin1    Peptidyl-prolyl cis/trans isomerases (PPIases) catalyze the cis/trans isomerization of peptidyl-prolyl peptide bonds. This gene encodes one of the PPIases, which specifically binds to phosphorylated ser/thr-pro motifs to catalytically regulate the post-phosphorylation conformation of its substrates. The conformational regulation catalyzed by this PPIase has a profound impact on key proteins involved in the regulation of cell growth, genotoxic and other stress responses, the immune response, induction and maintenance of pluripotency, germ cell development, neuronal differentiation, and survival. This enzyme also plays a key role in the pathogenesis of Alzheimer's disease and many cancers. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jun 2011]
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 UniProt Comments for Pin1    Peptidyl-prolyl cis/trans isomerase (PPIase) that binds to and isomerizes specific phosphorylated Ser/Thr-Pro (pSer/Thr-Pro) motifs. By inducing conformational changes in a subset of phosphorylated proteins, acts as a molecular switch in multiple cellular processes (PubMed:21497122, PubMed:22033920, PubMed:23623683). Displays a preference for acidic residues located N-terminally to the proline bond to be isomerized. Regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Down-regulates kinase activity of BTK (PubMed:16644721). Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation. Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation (PubMed:15664191). Binds and targets PML and BCL6 for degradation in a phosphorylation-dependent manner (PubMed:17828269). Acts as a regulator of JNK cascade by binding to phosphorylated FBXW7, disrupting FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation: degradation of FBXW7 leads to subsequent stabilization of JUN (PubMed:22608923). May facilitate the ubiquitination and proteasomal degradation of RBBP8/CtIP through CUL3/KLHL15 E3 ubiquitin-protein ligase complex, hence favors DNA double-strand repair through error-prone non-homologous end joining (NHEJ) over error-free, RBBP8-mediated homologous recombination (HR) (PubMed:23623683, PubMed:27561354).
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Product References and Citations for anti-Pin1 antibody

   Wulf GM, et al., (2002) J Biol. Chem. 277 (50)47976-47979; Hamdane M, et al., (2002) J Mol Neurosci. 19 (3) ; 275-287; Zheng H, et al., (2002) Nature 419 (6909) 853-857 Lu KP. et al., (1996) Nature 380 (6574) 544-547.; Campbell HD, et al., (1997) Genomics 44 (2), 157-162
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 Research Articles on Pin1    1. Parallel folding pathways of PIN1 Fip35 WW domain have been explained by infrared spectra and their computer simulations.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-Pin1 antibodyPathways associated with anti-Pin1 antibody
 Reference Product  PubMed Publications
 MYC antibody  >37 publications with Pin1 and MYC
 TP53 antibody  >11 publications with Pin1 and TP53
 CDK1 antibody  >10 publications with Pin1 and CDK1
 CCNB1 antibody  >9 publications with Pin1 and CCNB1
 IRF3 antibody  >8 publications with Pin1 and IRF3
 MYT1 antibody  >6 publications with Pin1 and MYT1
 RAF1 antibody  >4 publications with Pin1 and RAF1
 BRAF antibody  >3 publications with Pin1 and BRAF
 AXIN1 antibody  >1 publications with Pin1 and AXIN1
 PPP2CA antibody  >1 publications with Pin1 and PPP2CA
 Products by Pathway  Pathway Diagram
 Antiviral Mechanism By IFN-stimulated Genes Pathway antibodies  Antiviral Mechanism By IFN-stimulated Genes Pathway Diagram
 C-MYC Pathway antibodies  C-MYC Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 ISG15 Antiviral Mechanism Pathway antibodies  ISG15 Antiviral Mechanism Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Interferon Signaling Pathway antibodies  Interferon Signaling Pathway Diagram
 Negative Regulators Of RIG-I/MDA5 Signaling Pathway antibodies  Negative Regulators Of RIG-I/MDA5 Signaling Pathway Diagram
Diseases associated with anti-Pin1 antibodyOrgans/Tissues associated with anti-Pin1 antibody
 Disease Name  Pubmed Publications
 Neurodegenerative Diseases Antibodies  >127 publications with Pin1 and Neurodegenerative Diseases
 Nervous System Diseases Antibodies  >122 publications with Pin1 and Nervous System Diseases
 Brain Diseases Antibodies  >117 publications with Pin1 and Brain Diseases
 Breast Neoplasms Antibodies  >76 publications with Pin1 and Breast Neoplasms
 Cell Transformation, Neoplastic Antibodies  >53 publications with Pin1 and Cell Transformation, Neoplastic
 Liver Diseases Antibodies  >37 publications with Pin1 and Liver Diseases
 Inflammation Antibodies  >31 publications with Pin1 and Inflammation
 Carcinoma, Hepatocellular Antibodies  >29 publications with Pin1 and Carcinoma, Hepatocellular
 Cardiovascular Diseases Antibodies  >28 publications with Pin1 and Cardiovascular Diseases
 Neoplasm Metastasis Antibodies  >22 publications with Pin1 and Neoplasm Metastasis
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >118 publications with Pin1 and Brain
 Blood Antibodies  >76 publications with Pin1 and Blood
 Liver Antibodies  >46 publications with Pin1 and Liver
 Lung Antibodies  >42 publications with Pin1 and Lung
 Prostate Antibodies  >30 publications with Pin1 and Prostate
 Nerve Antibodies  >29 publications with Pin1 and Nerve
 Muscle Antibodies  >26 publications with Pin1 and Muscle
 Kidney Antibodies  >25 publications with Pin1 and Kidney
 Bone Antibodies  >24 publications with Pin1 and Bone
 Embryonic Tissue Antibodies  >21 publications with Pin1 and Embryonic Tissue
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