Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Antibody rightarrow RASSF1A rightarrow Monoclonal RASSF1A  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Nuclear factor of activated T-cells 3 (NFATC3) ELISA Kit
 • EGFR (Epidermal Growth Factor Receptor) Antibody
 • Nitrotyrosine (NOS2) Antibody
 • CD44v6 Antibody
 • HSP-70 ELISA Kit
 • Insulin receptor (INSR) Antibody
 • PINP ELISA Kit
 • S100 Calcium Binding Protein B (S100B) Antibody
 • Soytone Culture Media
 • H (+)-Cl (-) exchange transporter 3 (CLCN3) Recombinant Protein
 • Ornithine Decarboxylase-1 (ODC-1) (ODC1) Antibody
 • Troponin I (skTnI) Antibody
 • a-L-iduronidase (IDUA) ELISA Kit
 • Tubulin, alpha (TUBA) Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

anti-RASSF1A antibody :: Mouse anti-Human RASSF1A Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS200009
Unit / Price
0.05 mL  /  $240 +1 FREE 8GB USB
0.1 mL  /  $315 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

RASSF1A, Monoclonal Antibody

 Also Known As   

RASSF1A antibody

 Product Synonym Names    RASSF1 (Ras association (RalGDS/AF-6) domain family 1),123F2; RDA32; NORE2A; RASSF1A; REH3P21; Ras association (RalGDS/AF-6) domain family member 1;
 Product Gene Name   

anti-RASSF1A antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 OMIM    AF061836 mRNA
 3D Structure    ModBase 3D Structure for Q9NS23
Section Bar
 Clonality    Monoclonal
 Isotype    IgG1, k
 Clone Number    3F3
 Host    Mouse
 Species Reactivity    Human
Section Bar
 Purity/Purification    By protein-G affinity chromatography
 Form/Format    Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
 Concentration    1mg/ml (lot specific)
Section Bar
 Antigen Species    Human
 Immunogen    Recombinant human RASSF1A (1-340aa) purified from E. coli
 Clone Note    Anti-human RASSF1A mAb, clone 3F3, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant RASSF1A.
Section Bar
 Preparation and Storage    Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
 Other Notes    Small volumes of anti-RASSF1A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 

Related Product Information for anti-RASSF1A antibody

   The tumor suppressor gene RASSF1A (3p21.3), human Ras association domain family 1A, is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, small intestine, colon, and peripheral blood leukocytes. RASSF1A expression is inhibited by hypermethylation of the CpG island including the promoter and early transcribed regions of the human RASSF1A gene. Hypermethylated RASSF1A promoter is observed in primary tumors. Furthermore, RASSF1A is related with cell cycle regulation, apoptosis and microtubule stability.
 Product Categories/Family for anti-RASSF1A antibody    Cancer
 Applications Tested/Suitable for anti-RASSF1A antibody   

ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

Section Bar
 Western Blot (WB) of anti-RASSF1A antibody    Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RASSF1A (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate The Cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RASSF1A antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: RASSF1A Transfected 293T cell lysate
anti-RASSF1A antibody Western Blot (WB) (WB) image
Section Bar
 Immunofluorescence (IF) of anti-RASSF1A antibody    A431 cells were labeled with anti-RASSF1A (clone 3F3) mAb, and detection was using a biotinylated secondary antibody and Texas-red conj streptavidin. ICC/IF analysis of RASSF1A in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human RASSF1A antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
anti-RASSF1A antibody Immunofluorescence (IF) image
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for RASSF1A. It may not necessarily be applicable to this product.
 NCBI GI #    20149588
 NCBI GeneID    11186
 NCBI Accession #    NP_009113 [Other Products]
 NCBI GenBank Nucleotide #    NM_007182.4 [Other Products]
Section Bar
 UniProt Primary Accession #    Q9NS23 [Other Products]
 UniProt Secondary Accession #    O14571; O60539; O60710; Q0VGC6; Q5TZT2; Q9HB04; Q9HB18; Q9NS22; Q9UND4; Q9UND5; B7ZLL1 [Other Products]
 UniProt Related Accession #    Q9NS23 [Other Products]
 Molecular Weight    8,656 Da
Table BarTOPTable Bar
 NCBI Official Full Name    ras association domain-containing protein 1 isoform A
 NCBI Official Synonym Full Names    Ras association domain family member 1
 NCBI Official Symbol    RASSF1 [Similar Products]
 NCBI Official Synonym Symbols   
123F2; RDA32; NORE2A; RASSF1A; REH3P21
[Similar Products]
 NCBI Protein Information    ras association domain-containing protein 1
 UniProt Protein Name    Ras association domain-containing protein 1
 UniProt Gene Name    RASSF1 [Similar Products]
Section Bar
 NCBI Summary for RASSF1A    This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Several alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, May 2011]
Section Bar
 UniProt Comments for RASSF1A    Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage.
Table BarTOPTable Bar
 

Product References and Citations for anti-RASSF1A antibody

   Agathanggelou A, et al., (2005) Cancer Res. 65, 3497-3508; Lerman MI. et. al. (2000) Cancer Res. 60, 6116-6133; Strunnikova M. et al., (2005) Mol Cell Biol. 25, 3923-3933; Dammann R. et al., (2005) Histol Histopathol. 20, 645-663; Rossa W.k, et al., (2007) American Journal of pathology. 170, 941-950
Section Bar
 Research Articles on RASSF1A    1. Data show that silencing DNA methyltransferase 1 (DNMT1) increased expression of tumor suppressor genes, RASSF1A and DAPK, in esophageal squamous cell carcinoma (ESCC) cells and ESCC xenograft in nude mice.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with anti-RASSF1A antibodyPathways associated with anti-RASSF1A antibody
 Reference Product  PubMed Publications
 MLH1 antibody  >38 publications with RASSF1A and MLH1
 RASSF5 antibody  >37 publications with RASSF1A and RASSF5
 STK4 antibody  >16 publications with RASSF1A and STK4
 STK3 antibody  >14 publications with RASSF1A and STK3
 HRAS antibody  >8 publications with RASSF1A and HRAS
 CCND1 antibody  >7 publications with RASSF1A and CCND1
 MOAP1 antibody  >7 publications with RASSF1A and MOAP1
 MDM2 antibody  >7 publications with RASSF1A and MDM2
 NRAS antibody  >5 publications with RASSF1A and NRAS
 Products by Pathway  Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 Hippo Signaling Pathway -multiple Species antibodies  Hippo Signaling Pathway -multiple Species Diagram
 Hippo Signaling Pathway -multiple Species antibodies  Hippo Signaling Pathway -multiple Species Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 Non-small Cell Lung Cancer Pathway antibodies  Non-small Cell Lung Cancer Pathway Diagram
Diseases associated with anti-RASSF1A antibodyOrgans/Tissues associated with anti-RASSF1A antibody
 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >226 publications with RASSF1A and Adenocarcinoma
 Lung Neoplasms Antibodies  >212 publications with RASSF1A and Lung Neoplasms
 Breast Neoplasms Antibodies  >166 publications with RASSF1A and Breast Neoplasms
 Lung Diseases Antibodies  >162 publications with RASSF1A and Lung Diseases
 Carcinoma, Non-Small-Cell Lung Antibodies  >90 publications with RASSF1A and Carcinoma, Non-Small-Cell Lung
 Liver Neoplasms Antibodies  >76 publications with RASSF1A and Liver Neoplasms
 Neoplasms, Experimental Antibodies  >74 publications with RASSF1A and Neoplasms, Experimental
 Liver Diseases Antibodies  >69 publications with RASSF1A and Liver Diseases
 Carcinoma, Hepatocellular Antibodies  >62 publications with RASSF1A and Carcinoma, Hepatocellular
 Cell Transformation, Neoplastic Antibodies  >40 publications with RASSF1A and Cell Transformation, Neoplastic
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >231 publications with RASSF1A and Lung
 Blood Antibodies  >164 publications with RASSF1A and Blood
 Liver Antibodies  >86 publications with RASSF1A and Liver
 Prostate Antibodies  >75 publications with RASSF1A and Prostate
 Lymph Antibodies  >56 publications with RASSF1A and Lymph
 Lymph Node Antibodies  >56 publications with RASSF1A and Lymph Node
 Kidney Antibodies  >50 publications with RASSF1A and Kidney
 Brain Antibodies  >35 publications with RASSF1A and Brain
 Stomach Antibodies  >29 publications with RASSF1A and Stomach
 Skin Antibodies  >20 publications with RASSF1A and Skin
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions