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anti-POLR2A antibody :: Mouse RNA Polymerase II Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS680017 anti-POLR2A antibody
Unit / Price
0.1 mg  /  $380 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

RNA Polymerase II (POLR2A), Monoclonal Antibody

 Also Known As   

mAb to RNA Polymerase II

 Product Synonym Names    MAb to RPB1 subunit of Yeast RNA polymerase II; RNA Pol II RPB1 Monoclonal Antibody; RNA polymerase II sub-unit 1; RPB1; CTD repeat YSPTSPS; C- terminal domain repeat YSPTSPS; DNA-directed RNA polymerase II subunit A; RNA-directed RNA polymerase II subunit RPB1; POLR2A; POLR2
 Product Gene Name   

anti-POLR2A antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    YSPTSPS repeat
 Chromosome Location    Chromosome: 17; NC_000017.10 (7387698..7417935). Location: 17p13.1
 OMIM    180660
 3D Structure    ModBase 3D Structure for P24928
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 Clonality    Monoclonal
 Isotype    IgG2a
 Clone Number    8WG16
 Host    Mouse
 Species Reactivity    Yeast, Wheat, Human, most RNAPII
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 Specificity    RNA Polymerase II (RNA Pol II RPB1 human and yeast)
 Form/Format    Affinity Purified
 Concentration    1 mg/ml in PBS pH 7.2 (lot specific)
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 Immunogen    Purified protein
 Reported Reactivity    Wheat, mouse, C. elegans, X. laevis
 Cross Reactivity Note    Wheat, yeast, human, mouse, C. elegans, X. laevis and most eukaryotic RNAPII
 Polyol-responsive    Yes
 Reconstitution    Add 200ul of ddH2O to the tube containing antibody and mix to dissolve
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 Preparation and Storage    Store at -20 degree C lyophilized, 4 degree C Reconstituted, or sub-aliquot and store at -20 degree C
 Other Notes    Small volumes of anti-POLR2A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-POLR2A antibody

   MAb to RPB1 subunit of Yeast RNA polymerase II. RPB1 is a 220 kDA subunit of the yeast RNA polymerase II.
RPB1 is a 220kDa sub-unit of RNA Polymerase II
RPB1 is a 220 kDA subunit of the yeast RNA polymerase II.

RPB1 is the largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. It forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as a RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome.
 Applications Tested/Suitable for anti-POLR2A antibody   

Western Blot (WB), ChIP, IAC, ELISA (EIA), Transcription Inhibition, Immunoprecipitation (IP)

 Application Notes for anti-POLR2A antibody    Immunofluorescence (IF): Yes
Immunoaffinity Chromatography: Yes, polyol/NaCl
Immunoprecipitation (IP): Yes
Transcription Inhibition - Initiation: Yes
Western Blot: Yes
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NCBI/Uniprot data below describe general gene information for POLR2A. It may not necessarily be applicable to this product.
 NCBI GI #    4505939
 NCBI GeneID    5430
 NCBI Accession #    NP_000928 [Other Products]
 NCBI GenBank Nucleotide #    NM_000937.4 [Other Products]
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 UniProt Primary Accession #    P24928 [Other Products]
 UniProt Secondary Accession #    A6NN93; B9EH88 [Other Products]
 UniProt Related Accession #    P24928 [Other Products]
 Molecular Weight    217,176 Da [Similar Products]
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 NCBI Official Full Name    DNA-directed RNA polymerase II subunit RPB1
 NCBI Official Synonym Full Names    polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
 NCBI Official Symbol    POLR2A [Similar Products]
 NCBI Official Synonym Symbols   
RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220
[Similar Products]
 NCBI Protein Information    DNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; DNA-directed RNA polymerase II subunit A; RNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit
 UniProt Protein Name    DNA-directed RNA polymerase II subunit RPB1
 UniProt Synonym Protein Names   
DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA-directed RNA polymerase II subunit RPB1
 Protein Family    RNA polymerase II assembly factor
 UniProt Gene Name    POLR2A [Similar Products]
 UniProt Synonym Gene Names    POLR2; RNA polymerase II subunit B1 [Similar Products]
 UniProt Entry Name    RPB1_HUMAN
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 NCBI Summary for POLR2A    This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq, Jul 2008]
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 UniProt Comments for POLR2A    Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as a RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome. Ref.6 Ref.23

Catalytic activity: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Subunit structure: Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. The phosphorylated C-terminal domain interacts with FNBP3 and SYNCRIP. Interacts with SAFB/SAFB1. Interacts with CCNL1 and MYO1C

By similarity. Interacts with CCNL2 and SFRS19. Component of a complex which is at least composed of HTATSF1/Tat-SF1, the P-TEFb complex components CDK9 and CCNT1, RNA polymerase II, SUPT5H, and NCL/nucleolin. Interacts with PAF1. Interacts (via C-terminus) with FTSJD2, CTDSP1 and SCAF8. Interacts via the phosphorylated C-terminal domain with WDR82 and with SETD1A and SETD1B only in the presence of WDR82. Interacts with ATF7IP. When phosphorylated at 'Ser-5', interacts with MEN1; the unphosphorylated form, or phosphorylated at 'Ser-2' does not interact. Interacts with DDX5. Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.14 Ref.15 Ref.16 Ref.17 Ref.18 Ref.20 Ref.21 Ref.22 Ref.24 Ref.26

Subcellular location: Nucleus Ref.6.

Post-translational modification: The tandem 7 residues repeats in the C-terminal domain (CTD) can be highly phosphorylated. The phosphorylation activates Pol II. Phosphorylation occurs mainly at residues 'Ser-2' and 'Ser-5' of the heptapeptide repeat and is mediated, at least, by CDK7 and CDK9. CDK7 phosphorylation of POLR2A associated with DNA promotes transcription initiation by triggering dissociation from DNA. Phosphorylation also takes place at 'Ser-7' of the heptapepdtide repeat, which is required for efficient transcription of snRNA genes and processing of the transcripts. The phosphorylation state is believed to result from the balanced action of site-specific CTD kinases and phosphatases, and a 'CTD code' that specifies the position of Pol II within the transcription cycle has been proposed. Ref.27 Ref.28 Ref.29 Ref.33 Ref.36 Ref.38Dephosphorylated by the protein phosphatase CTDSP1. Ref.27 Ref.28 Ref.29 Ref.33 Ref.36 Ref.38Ubiquitinated by WWP2 leading to proteasomal degradation

By similarity. Following UV treatment, the elongating form of RNA polymerase II (RNA pol IIo) is ubiquitinated UV damage sites without leading to degradation: ubiquitination is facilitated by KIAA1530/UVSSA and promotes RNA pol IIo backtracking to allow access to the nucleotide excision repair machinery. Ref.37Methylated at Arg-1810 by CARM1. Methylation occurs only when the CTD is hypophosphorylated, and phosphorylation at Ser-1805 and Ser-1808 prevent methylation (in vitro). It is assumed that methylation occurs prior to phosphorylation and transcription initiation. CTD methylation may facilitate the expression of select RNAs. Ref.27 Ref.28 Ref.29 Ref.33 Ref.36 Ref.38

Miscellaneous: The binding of ribonucleoside triphosphate to the RNA polymerase II transcribing complex probably involves a two-step mechanism. The initial binding seems to occur at the entry (E) site and involves a magnesium ion temporarily coordinated by three conserved aspartate residues of the two largest RNA Pol II subunits. The ribonucleoside triphosphate is transferred by a rotation to the nucleotide addition (A) site for pairing with the template DNA. The catalytic A site involves three conserved aspartate residues of the RNA Pol II largest subunit which permanently coordinate a second magnesium ion.

Sequence similarities: Belongs to the RNA polymerase beta' chain family.
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 Research Articles on POLR2A    1. inhibition of the transition of paused RNA PolII to productive elongation, described here for p21(CIP1), is a general mechanism by which transcription factor Sp3 fine-tunes gene expression.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-POLR2A antibodyDiseases associated with anti-POLR2A antibody
 Products by Pathway  Pathway Diagram
 Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway antibodies  Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Dual Incision Reaction In TC-NER Pathway antibodies  Dual Incision Reaction In TC-NER Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Eukaryotic Transcription Initiation Pathway antibodies  Eukaryotic Transcription Initiation Pathway Diagram
 Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway antibodies  Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway Diagram
 Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway antibodies  Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway Diagram
 Formation Of RNA Pol II Elongation Complex Pathway antibodies  Formation Of RNA Pol II Elongation Complex Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >14 publications with POLR2A and Neoplasms
 Cardiovascular Diseases Antibodies  >4 publications with POLR2A and Cardiovascular Diseases
 Adenocarcinoma Antibodies  >4 publications with POLR2A and Adenocarcinoma
 Disease Models, Animal Antibodies  >3 publications with POLR2A and Disease Models, Animal
 Adenoma Antibodies  >2 publications with POLR2A and Adenoma
 Colonic Neoplasms Antibodies  >1 publications with POLR2A and Colonic Neoplasms
 Stomach Neoplasms Antibodies  >1 publications with POLR2A and Stomach Neoplasms
 Poisoning Antibodies  >1 publications with POLR2A and Poisoning
 Drug Toxicity Antibodies  >1 publications with POLR2A and Drug Toxicity
 Inflammation Antibodies  >1 publications with POLR2A and Inflammation
Organs/Tissues associated with anti-POLR2A antibody
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >7 publications with POLR2A and Brain
 Heart Antibodies  >5 publications with POLR2A and Heart
 Liver Antibodies  >4 publications with POLR2A and Liver
 Lung Antibodies  >4 publications with POLR2A and Lung
 Kidney Antibodies  >3 publications with POLR2A and Kidney
 Muscle Antibodies  >3 publications with POLR2A and Muscle
 Adipose Tissue Antibodies  >2 publications with POLR2A and Adipose Tissue
 Connective Tissue Antibodies  >2 publications with POLR2A and Connective Tissue
 Stomach Antibodies  >1 publications with POLR2A and Stomach
 Embryonic Tissue Antibodies  >1 publications with POLR2A and Embryonic Tissue
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