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anti-RPA1 antibody :: Rat RPA, p70 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS604298 anti-RPA1 antibody
Unit / Price
0.1 mL  /  $540 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

RPA, p70 (RPA1), Monoclonal Antibody

 Also Known As   

RPA, p70 (DNA Replication, Repair and Recombination Factor)

 Product Synonym Names    Anti -RPA, p70 (DNA Replication, Repair and Recombination Factor)
 Product Gene Name   

anti-RPA1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 17; NC_000017.10 (1733273..1802848). Location: 17p13.3
 OMIM    179835
 3D Structure    ModBase 3D Structure for P27694
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 Clonality    Monoclonal
 Clone Number    7H59
 Host    Rat
 Species Reactivity    Human, Monkey
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 Specificity    Detects endogenous levels of total RPA70 protein. Species Crossreactivity: Human and monkey.
 Purity/Purification    Serum
Serum.
 Form/Format    Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol and less than 0.02% sodium azide.
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 Immunogen    Recombinant MBP-fusion protein corresponding to the carboxy-terminal sequence of human.
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 Preparation and Storage    May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
 Other Notes    Small volumes of anti-RPA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-RPA1 antibody

   RPA70 (HSSB, REPA1, RF-A, RP-A, p70) is a component of a heterotrimeric complex, composed of 70, 32/30 and 14kD subunits, collectively known as RPA. RPA is a single stranded DNA binding protein, whose DNA binding activity is believed to reside entirely in the 70kD subunit. The complex is required for almost all aspects of cellular DNA metabolism such as DNA replication (1,2,3), recombination, cell cycle and DNA damage checkpoints, and all major types of DNA repair including nucleotide excision, base excision, mismatch and double-strand break repairs (4,5,6,7). In response to genotoxic stress in eukaryotic cells, RPA has been shown to associate with the Rad9/Rad1/Hus1 (9-1-1) checkpoint complex (8). RPA is hyperphosphorylated upon DNA damage or replication stress by checkpoint kinases including ataxia telangiectasia mutated (ATM), ATM and Rad3-related (ATR), and DNA-dependent protein kinase (DNA-PK) (9,10,11). Hyperphosphorylation may alter RPA-DNA and RPA-protein interactions. In addition to the checkpoint partners, RPA interacts with a wide variety of protein partners, including proteins required for normal replication such as RCF, PCNA and Pol, and also proteins involved in SV40 replication, such as DNA polymerase I and SV40 large T antigen (12,10).
 Product Categories/Family for anti-RPA1 antibody    Antibodies; Abs to DNA Replication, Repair
 Applications Tested/Suitable for anti-RPA1 antibody   

ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

 Application Notes for anti-RPA1 antibody    Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation.
Dilution: Western blot 1:1000
Immunoprecipitation 1:50
Immunofluorescence (IF-IC) 1:100
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NCBI/Uniprot data below describe general gene information for RPA1. It may not necessarily be applicable to this product.
 NCBI GI #    4506583
 NCBI GeneID    6117
 NCBI Accession #    NP_002936.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_002945.3 [Other Products]
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 UniProt Primary Accession #    P27694 [Other Products]
 UniProt Secondary Accession #    Q59ES9; A8K0Y9 [Other Products]
 UniProt Related Accession #    P27694; Q7Z4G2 [Other Products]
 Molecular Weight    68,138 Da [Similar Products]
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 NCBI Official Full Name    replication protein A 70 kDa DNA-binding subunit
 NCBI Official Synonym Full Names    replication protein A1, 70kDa
 NCBI Official Symbol    RPA1 [Similar Products]
 NCBI Official Synonym Symbols   
HSSB; RF-A; RP-A; REPA1; RPA70; MST075
[Similar Products]
 NCBI Protein Information    replication protein A 70 kDa DNA-binding subunit; MSTP075; RP-A p70; RF-A protein 1; OTTHUMP00000115691; replication factor A protein 1; single-stranded DNA-binding protein
 UniProt Protein Name    Replication protein A 70 kDa DNA-binding subunit
 UniProt Synonym Protein Names   
Replication factor A protein 1; RF-A protein 1; Single-stranded DNA-binding protein
 Protein Family    Replication factor
 UniProt Gene Name    RPA1 [Similar Products]
 UniProt Synonym Gene Names    REPA1; RPA70 [Similar Products]
 UniProt Entry Name    RFA1_HUMAN
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 UniProt Comments for RPA1    RPA1: Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing. Heterotrimer composed of RPA1, RPA2 and RPA3 (canonical replication protein A complex). Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. The DNA-binding activity may reside exclusively on the RPA1 subunit. Interacts with RIPK1 and XPA. Interacts with RPA4. Interacts with the polymerase alpha subunit POLA1/p180; this interaction stabilizes the replicative complex and reduces the misincorporation rate of DNA polymerase alpha by acting as a fidelity clamp. Interact with RAD51 and SENP6 to regulate DNA repair. Interacts with HELB; this interaction promotes HELB recruitement to chromatin following DNA damage. Belongs to the replication factor A protein 1 family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: male germ cell nucleus; nucleoplasm; lateral element; PML body; DNA replication factor A complex; nucleus

Molecular Function: protein binding; metal ion binding; damaged DNA binding; chromatin binding; single-stranded DNA binding

Biological Process: meiosis; mismatch repair; in utero embryonic development; DNA strand elongation during DNA replication; DNA repair; double-strand break repair via homologous recombination; DNA recombination; telomere maintenance via semi-conservative replication; DNA recombinase assembly; homeostasis of number of cells within a tissue; nucleotide-excision repair; base-excision repair; double-strand break repair; transcription-coupled nucleotide-excision repair; positive regulation of cell proliferation; telomere maintenance via recombination; nucleotide-excision repair, DNA damage removal; hemopoiesis; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; DNA replication; DNA-dependent DNA replication; telomere maintenance; G1/S transition of mitotic cell cycle
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 Research Articles on RPA1    1. data suggest that hnRNPA1, TERRA and POT1 act in concert to displace RPA from telomeric ssDNA after DNA replication, and promote telomere capping to preserve genomic integrity
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-RPA1 antibodyPathways associated with anti-RPA1 antibody
 Reference Product  PubMed Publications
 XPA antibody  >59 publications with RPA1 and XPA
 PCNA antibody  >58 publications with RPA1 and PCNA
 RPA2 antibody  >47 publications with RPA1 and RPA2
 RAD51 antibody  >33 publications with RPA1 and RAD51
 RPA3 antibody  >24 publications with RPA1 and RPA3
 PRKDC antibody  >22 publications with RPA1 and PRKDC
 WRN antibody  >18 publications with RPA1 and WRN
 BLM antibody  >13 publications with RPA1 and BLM
 RAD52 antibody  >13 publications with RPA1 and RAD52
 ATRIP antibody  >10 publications with RPA1 and ATRIP
 Products by Pathway  Pathway Diagram
 Activation Of ATR In Response To Replication Stress Pathway antibodies  Activation Of ATR In Response To Replication Stress Pathway Diagram
 Activation Of The Pre-replicative Complex Pathway antibodies  Activation Of The Pre-replicative Complex Pathway Diagram
 Assembly Of The RAD51-ssDNA Nucleoprotein Complex Pathway antibodies  Assembly Of The RAD51-ssDNA Nucleoprotein Complex Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Chromosome Maintenance Pathway antibodies  Chromosome Maintenance Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Replication Pre-Initiation Pathway antibodies  DNA Replication Pre-Initiation Pathway Diagram
Diseases associated with anti-RPA1 antibodyOrgans/Tissues associated with anti-RPA1 antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >59 publications with RPA1 and Neoplasms
 Metabolic Diseases Antibodies  >50 publications with RPA1 and Metabolic Diseases
 Congenital Abnormalities Antibodies  >30 publications with RPA1 and Congenital Abnormalities
 Photosensitivity Disorders Antibodies  >23 publications with RPA1 and Photosensitivity Disorders
 Precancerous Conditions Antibodies  >23 publications with RPA1 and Precancerous Conditions
 Carcinoma Antibodies  >16 publications with RPA1 and Carcinoma
 Immune System Diseases Antibodies  >16 publications with RPA1 and Immune System Diseases
 Leukemia Antibodies  >9 publications with RPA1 and Leukemia
 Cardiovascular Diseases Antibodies  >9 publications with RPA1 and Cardiovascular Diseases
 Drug Toxicity Antibodies  >8 publications with RPA1 and Drug Toxicity
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >18 publications with RPA1 and Blood
 Skin Antibodies  >7 publications with RPA1 and Skin
 Lung Antibodies  >4 publications with RPA1 and Lung
 Ovary Antibodies  >4 publications with RPA1 and Ovary
 Liver Antibodies  >4 publications with RPA1 and Liver
 Brain Antibodies  >4 publications with RPA1 and Brain
 Testis Antibodies  >3 publications with RPA1 and Testis
 Placenta Antibodies  >3 publications with RPA1 and Placenta
 Thymus Antibodies  >3 publications with RPA1 and Thymus
 Bone Antibodies  >3 publications with RPA1 and Bone
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