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anti-RPA14 antibody :: Mouse RPA14 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS190409 anti-RPA14 antibody
Unit / Price
0.1 mg  /  $410 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

RPA14, Monoclonal Antibody

 Also Known As   

RPA14 Monoclonal Antibody

 Product Synonym Names    Anti-RPA14; RPA14
 Product Gene Name   

anti-RPA14 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: IV; NC_001136.10 (769525..769938)
 3D Structure    ModBase 3D Structure for P50106
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 Clonality    Monoclonal
 Isotype    IgG1 kappa
 Host    Mouse
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 Specificity    Anti-RPA14 [Murine MAb recognizes Replication Protein A (14 kD), a single-stranded DNA binding protein essential in DNA replication, homologous recombination, and nucleotide excision repair.]
This antibody recognizes human and mouse RPA14. RPA (Replication Protein A) is a single-stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 70, 32, and 14 kD. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination, and nucleotide excision repair.
 Form/Format    100 ug Protein G-purified antibody in PBS, pH 7.4
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 Antigen    N-terminally 6x-His tagged fusion protein corresponding to fulllength human RPA14 expressed in E. coli.
 Stabilizer    None
 Preservatives    None. Available on request
 Dilutions Instructions    Dilute in PBS or medium which is identical to that used in the assay system.
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 Preparation and Storage    This antibody is stable for at least one (1) year at -70 degree C. Avoid multiple freeze thaw cycles.
 Other Notes    Small volumes of anti-RPA14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for anti-RPA14 antibody    Cancer Research Products
 Applications Tested/Suitable for anti-RPA14 antibody   

Immunoblot, Immunoprecipitation

 Application Notes for anti-RPA14 antibody    Immunoblotting, immunoprecipitation: use at 0.2-1 ug/ml.
Positive control: HeLa, Raji, and 3T3 cells and recombinant protein.
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NCBI/Uniprot data below describe general gene information for RPA14. It may not necessarily be applicable to this product.
 NCBI GI #    6320360
 NCBI GeneID    851734
 NCBI Accession #    NP_010440.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001180463.1 [Other Products]
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 UniProt Primary Accession #    P50106 [Other Products]
 UniProt Secondary Accession #    D6VSD7 [Other Products]
 UniProt Related Accession #    P50106 [Other Products]
 Molecular Weight    14,585 Da [Similar Products]
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 NCBI Official Full Name    Rpa14p
 NCBI Official Symbol    RPA14 [Similar Products]
 NCBI Protein Information    Rpa14p
 UniProt Protein Name    DNA-directed RNA polymerase I subunit RPA14
 UniProt Synonym Protein Names   
DNA-directed RNA polymerase I 14 kDa polypeptide
 Protein Family    DNA-directed RNA polymerase
 UniProt Gene Name    RPA14 [Similar Products]
 UniProt Entry Name    RPA14_YEAST
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 UniProt Comments for RPA14    Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. A14 seems to play a role in the stability of subunits ABC23 and A43. In vitro, the A14-A43 subcomplex binds single-stranded RNA. Ref.12

Subunit structure: Component of the RNA polymerase I (Pol I) complex consisting of 14 subunits. Part of a Pol I subcomplex consisting of the subunits A14 and A43. Interacts with RPA43. Ref.9

Subcellular location: Nucleus › nucleolus Ref.10.

Post-translational modification: The N-terminus is blocked.

Miscellaneous: Present with 3100 molecules/cell in log phase SD medium. Ref.11
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Product References and Citations for anti-RPA14 antibody

   Iftode, C et al. 1999. Crit Rev Biochem Mol Biol 34: 141-180; Bochkarev, A et al. 1999. EMBO 18: 4498-4504; Dong, J et al. 1999. Biochem J 337: 311-317; Sibenaller, ZA et al. 1998. Biochemistry 37: 12496-12506; Stigger, E et al. 1998. J Biol Chem 273: 9337- 9343.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-RPA14 antibody
 Products by Pathway  Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Pyrimidine Metabolism Pathway antibodies  Pyrimidine Metabolism Pathway Diagram
 Pyrimidine Metabolism Pathway antibodies  Pyrimidine Metabolism Pathway Diagram
 RNA Polymerase Pathway antibodies  RNA Polymerase Pathway Diagram
 RNA Polymerase Pathway antibodies  RNA Polymerase Pathway Diagram
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