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anti-RSPO1 antibody :: Rat anti-Mouse RSPO-3 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS603109 anti-RSPO1 antibody
Unit / Price
0.1 mg  /  $605 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

RSPO-3 (RSPO1), Monoclonal Antibody

 Also Known As   

RSPO-3 (R-Spondin 3, Cristin-1)

 Product Synonym Names    Anti -RSPO-3 (R-Spondin 3, Cristin-1)
 Product Gene Name   

anti-RSPO1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 1; NC_000001.10 (38076821..38100595, complement). Location: 1p34.3
 OMIM    609595
 3D Structure    ModBase 3D Structure for Q2MKA7
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 Clonality    Monoclonal
 Isotype    IgG2a
 Clone Number    7H222
 Host    Rat
 Species Reactivity    Mouse
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 Specificity    Recognizes mouse RSPO-3. No crossreactivity with recombinant mouse RSPO-1, RSPO-4, recombinant human RSPO-2 or RSPO-3.
 Purity/Purification    Affinity Purified
Purified by Protein G affinity chromatography.
 Form/Format    Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS, pH 7.4.
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 Immunogen    Recombinant corresponding to aa21-209 of mouse RSPO-3 expressed in E. coli (Accession #BAC36296).
 Hybridoma    Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a rat immunized with purified, E. coli-derived, recombinant mouse RSPO-3.
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 Preparation and Storage    Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
 Other Notes    Small volumes of anti-RSPO1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-RSPO1 antibody

   RSPO-3 (R-spondin 3; also Cristin-1), is a 256aa secreted R-Spondin family member which binds LRP-6 and Fzd8 and regulates b-catenin signaling. R-Spondins contain two adjacent cysteine-rich furin-like domains at their amino-termini, followed by a thrombospondin (TSP-1) motif and a variable length basic C-terminal region. Two RSPO-3 splice isoforms that differ in their C-termini have been reported. Mouse RSPO-3 shares 93% and 97% aa identity with human and rat RSPO-3, respectively, over the sequence used as an immunogen.
 Product Categories/Family for anti-RSPO1 antibody    Antibodies; Abs to Proteins
 Applications Tested/Suitable for anti-RSPO1 antibody   

ELISA (EL/EIA), Western Blot (WB)

 Application Notes for anti-RSPO1 antibody    Suitable for use in Direct ELISA and Western Blot.
Dilution: Western Blot: 1ug/ml
Optimal dilutions to be determined by researcher.
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NCBI/Uniprot data below describe general gene information for RSPO1. It may not necessarily be applicable to this product.
 NCBI GI #    339276103
 NCBI GeneID    284654
 NCBI Accession #    NP_001229839.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001242910.1 [Other Products]
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 UniProt Primary Accession #    Q2MKA7 [Other Products]
 UniProt Secondary Accession #    Q0H8S6; Q14C72; Q5T0F2; Q8N7L5; A2A420 [Other Products]
 UniProt Related Accession #    Q2MKA7 [Other Products]
 Molecular Weight    28,959 Da [Similar Products]
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 NCBI Official Full Name    R-spondin-1 isoform 3
 NCBI Official Synonym Full Names    R-spondin 1
 NCBI Official Symbol    RSPO1 [Similar Products]
 NCBI Official Synonym Symbols   
RSPO; CRISTIN3; RP11-566C13.1
[Similar Products]
 NCBI Protein Information    R-spondin-1; R-spondin homolog; roof plate-specific spondin-1
 UniProt Protein Name    R-spondin-1
 UniProt Synonym Protein Names   
Roof plate-specific spondin-1
 Protein Family    R-spondin
 UniProt Gene Name    RSPO1 [Similar Products]
 UniProt Synonym Gene Names    hRspo1 [Similar Products]
 UniProt Entry Name    RSPO1_HUMAN
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 NCBI Summary for RSPO1    This gene is a member of the R-spondin family and encodes a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. [provided by RefSeq, Jul 2008]
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 UniProt Comments for RSPO1    R-spondin-1: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Defects in RSPO1 are the cause of palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal (PKKSCC). This recessive syndrome is characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. Belongs to the R-spondin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Secreted, signal peptide; Secreted; Cell development/differentiation

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: extracellular space; extracellular region; nucleus

Molecular Function: heparin binding; protein binding; G-protein-coupled receptor binding; receptor binding

Biological Process: regulation of gene expression; regulation of receptor internalization; Wnt receptor signaling pathway through beta-catenin; positive regulation of protein amino acid phosphorylation; male meiosis; positive regulation of Wnt receptor signaling pathway

Disease: Palmoplantar Hyperkeratosis With Squamous Cell Carcinoma Of Skin And 46,xx Sex Reversal
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 Research Articles on RSPO1    1. In conclusion, present study highlights the role of Rspo 1 in bone remodeling where it activates Wnt signaling to induce differentiation, as shown in human as well murine in vitro osteoblast cell models.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-RSPO1 antibodyPathways associated with anti-RSPO1 antibody
 Reference Product  PubMed Publications
 SRY antibody  >31 publications with RSPO1 and SRY
 SOX9 antibody  >26 publications with RSPO1 and SOX9
 FOXL2 antibody  >15 publications with RSPO1 and FOXL2
 LRP6 antibody  >10 publications with RSPO1 and LRP6
 DKK1 antibody  >9 publications with RSPO1 and DKK1
 FURIN antibody  >6 publications with RSPO1 and FURIN
 ZNRF3 antibody  >4 publications with RSPO1 and ZNRF3
 STRA8 antibody  >2 publications with RSPO1 and STRA8
 MYF5 antibody  >1 publications with RSPO1 and MYF5
 Products by Pathway  Pathway Diagram
 Wnt Signaling Network Pathway antibodies  Wnt Signaling Network Pathway Diagram
Diseases associated with anti-RSPO1 antibodyOrgans/Tissues associated with anti-RSPO1 antibody
 Disease Name  Pubmed Publications
 Urogenital Abnormalities Antibodies  >21 publications with RSPO1 and Urogenital Abnormalities
 Neoplasms Antibodies  >10 publications with RSPO1 and Neoplasms
 Necrosis Antibodies  >2 publications with RSPO1 and Necrosis
 Musculoskeletal Abnormalities Antibodies  >2 publications with RSPO1 and Musculoskeletal Abnormalities
 Hyperplasia Antibodies  >2 publications with RSPO1 and Hyperplasia
 Hypertrophy Antibodies  >1 publications with RSPO1 and Hypertrophy
 Nervous System Diseases Antibodies  >1 publications with RSPO1 and Nervous System Diseases
 Liver Diseases Antibodies  >1 publications with RSPO1 and Liver Diseases
 Ovarian Diseases Antibodies  >1 publications with RSPO1 and Ovarian Diseases
 Adenocarcinoma Antibodies  >1 publications with RSPO1 and Adenocarcinoma
 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >30 publications with RSPO1 and Testis
 Ovary Antibodies  >26 publications with RSPO1 and Ovary
 Intestine Antibodies  >11 publications with RSPO1 and Intestine
 Pancreas Antibodies  >3 publications with RSPO1 and Pancreas
 Muscle Antibodies  >2 publications with RSPO1 and Muscle
 Connective Tissue Antibodies  >2 publications with RSPO1 and Connective Tissue
 Parathyroid Antibodies  >1 publications with RSPO1 and Parathyroid
 Heart Antibodies  >1 publications with RSPO1 and Heart
 Brain Antibodies  >1 publications with RSPO1 and Brain
 Prostate Antibodies  >1 publications with RSPO1 and Prostate
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