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Mouse Rainbow Trout Ig (H) Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS520483 anti-Rainbow Trout Ig (H) antibody
Unit / Price
0.25 mg  /  $390 +1 FREE 8GB USB
 
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 Product Name   

Rainbow Trout Ig (H), Monoclonal Antibody

★Popular Item★
 Also Known As   

Anti-Rainbow Trout Ig (H), Purified, (Clone IPA2C7) (mouse IgG1)

 Product Synonym Names    Rainbow Trout Ig (H), Purified, (Clone IPA2C7) (mouse IgG1); Purified Anti-Rainbow Trout Ig Monoclonal Antibody
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Monoclonal
 Isotype    Mouse IgG1
 Clone Number    IPA2C7
 Host    Mouse
 Species Reactivity    Rainbow Trout
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 Specificity    Trout lg (H) (Immunoglobin heavy chain in Rainbow trout species)
 Form/Format    Purified
 Concentration    Antibody Concentration: 1.0 mg/ml (lot specific)
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 Immunogen    Immunogen: Purified Rainbow trout Ig (heavy and light chains)
 Fusion Partner    X63Ag8.653 myloma cells
 Presentation    Purified Ig buffered in PBS with the addition of 0.02% NaN3 (PEG Purified from Ascitic Fluid)
 Method    ELISA METHOD

I. Prepare a stock solution of 10-20 flg/ml of virus antigens in carbonate coating buffer, pH 9.6 and add 100fll/well (ie 1.0-2.0flg of antigen/well). Incubate at 4C overnight. (For bacteria, dilute 1.0 00 660 nm of suspension 1/100 in water, add 100 fll per well and dry down overnight at 37C. If your antigen is fish serum, you must dilute I/IO-I/SO in carbonate buffer and add 100 fll per well.)
2. The next day, rinse plate 3 times quickly with PBS-0.05% tween 20 detergent, pH 7.4.
3. Block the plate (100 fll per well) with PBS, pH 7.4, containing 1-2% pre-immune goat serum. Incubate for I hour at room temperature.
4. Flick out the blocking buffer and rinse plate 3 times quickly with PBS-0.05% tween 20.
5. Add 100 fll per well of the salmon immune serum, diluted I/IO-I/SO in *PBS-0.05% tween 20. Incubate for 2 hours at room temperature or overnight at 4C for best results.
6. Wash plate 3 times with PBS-0.05% tween 20 quickly. Then wash 3 x 10 minutes with PBS-0.05% tween 20 (ie. fill up the wells and let plate sit for 10 minutes, repeat 2 times) This longer washing step will reduce background.
7. Do not let the plate sit dry for long before adding III 00-1 /40M dilution ofCLFOOIAP in *PBS-0.05% tween 20 to all the wells. Incubate for 2 hours at room temperature.
8. Wash as in step 6.
9. Do not let the plate sit dry long before adding 100 fll per well of goat anti-mouse IgG I-Alk. Phos. secondary antibody:~, diluted 111 000-1/2000 in PBS-0.05% tween 20-0.5% pre-immune goat serum. Incubate for 2 hours at room temperature or I hour at 37C.
10. Again, wash as in step 6.
II. Add 100 ul per well of A Ik. Phos. substrate (one Sigma 104-105 Alk. Phos. substrate tablet in 5ml Diethanolamine buffer, pH 9.6). Incubate for 30-65 minutes in the dark. Read plate at 405 nm.

*If background IS high, add 0.5% pre-Immune goat serum to the buffer solution.

For optimal results in various applications, it is recommended that each investigator determine dilutions appropriate for individual use. In ELISA and Western blot assays, the recommended dilution for maximum activity is 1/100.

It is recommended that the goat anti-mouse IgG 1 Alk. Phos. secondary antibody is used as this will increase the sensitivity 3-5 times above a normal multi-valent secondary antibody.
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 Preparation and Storage    Stable at 4 degree C. For long term storage, aliquot and freeze unused portions at -20 degree C in volumes appropriate for single usage. Avoid repeated freeze/thaw cycles.
 Other Notes    Small volumes of anti-Rainbow Trout Ig (H) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-Rainbow Trout Ig (H) antibody

   The anti-Rainbow trout Ig monoclonal antibody can be used to indirectly detect a generalized or pathogen-specific increase in immunoglobulin level. This monoclonal antibody only recognizes the immunoglobin heavy chain found in Oncorhynchus species (ie. Coho, Chum, Sockeye and Chinook) but not the Atlantic salmon heavy chain. This antibody can be used as a detection reagent for fish infection by either detecting a generalized increase in fish immunoglobulin level or by detecting specific antibody. For specific antibody detection, known infectious agents are used as the antigen in an ELISA assay, followed by samples of infected fish serum and then the anti-Rainbow Trout Ig monoclonal antibody. See ELISA protocol below. Specific uses for this monoclonal antibody include ELISA, Western blots and immunofluorescence (ie. FACS).
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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