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anti-CA9 antibody :: Mouse anti-Human, Rat Renal Cell Carcinoma Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS370104
Unit / Price
5 Control Slides  /  $130 +1 FREE 8GB USB
0.1 mL (Concentrate)  /  $170 +1 FREE 8GB USB
3 ml (Prediluted)  /  $185 +1 FREE 8GB USB
7 ml (Prediluted)  /  $240 +1 FREE 8GB USB
0.5 mL (Concentrate)  /  $250 +1 FREE 8GB USB
1 mL (Concentrate)  /  $395 +1 FREE 8GB USB
15 ml (Prediluted)  /  $395 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Renal Cell Carcinoma (CA9), Monoclonal Antibody

★Popular Item★
 Also Known As   

Renal Cell Carcinoma

 Product Gene Name   

anti-CA9 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 9; NC_000009.11 (35673915..35681156). Location: 9p13.3
 OMIM    603179
 3D Structure    ModBase 3D Structure for Q16790
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 Clonality    Monoclonal
 Isotype    IgG1/K
 Clone Number    PN-15
 Host    Mouse
 Species Reactivity    Human, Rat
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 Form/Format    Anti-Renal Cell Carcinoma is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
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 Control    Kidney, Renal Cell Carcinoma
 Localization    Cytoplasmic, Membranous
 Reactivity Note    Paraffin, Frozen
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 Preparation and Storage    Store at 2C - 8C
Stability: 3 years
 Other Notes    Small volumes of anti-CA9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-CA9 antibody

   Renal Cell Carcinoma, also known as a Gurnistical Tumor, is the most common form of kidney cancer arising from the renal tubule. It is also the most common type of kidney cancer in adults. Initial treatment is surgery because it is notoriously resistant to radiation therapy and chemotherapy, although some cases respond to immunotherapy. Renal Cell Carcinoma antibody recognizes a 200 kDa glycoprotein localized in the brush border of the proximal renal tubule. This antibody immunoreacts with approximately 90% of Primary Renal Cell Carcinomas and approximately 85% of Metastatic Renal Cell Carcinomas. Other tumors that may react with this antibody are Parathyroid Adenoma, an occasional Breast Carcinoma. Nephroblastoma, Oncocytoma, Mesoblastic Nephroma, Transitional Cell Carcinoma, and Angiomyolipoma are not labeled with this antibody.
 Application Notes for anti-CA9 antibody    Recommended IHC Protocol Pretreatment:
1. Cut and mount 3-4 micron formalin-fixed paraffin-embedded tissues on positive charged slides.
2. Air dry for 2 hours at 58C
3. Deparaffinize, dehydrate and rehydrate tissues.
4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA.
5. Any of three heating methods may be used:
a. Electric Pressure Cooker.
Place standoff rack at base of pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high, and incubate for 15 minutes. Open and immediately transfer slides to room temperature.
b. Water Bath Method.
Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a water bath set at 95-99 C. lncubate for 30-60 minutes.
c. Conventional Steamer Method.
Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes. .
6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.
7. Wash slides with IHC wash buffer or DI water.
8. Continue IHe staining protocol.!!
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 Dilution Information of anti-CA9 antibody    anti-CA9 antibody Dilution Information image
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 Immunohistochemistry (IHC) of anti-CA9 antibody    anti-CA9 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) Protocol of anti-CA9 antibody    anti-CA9 antibody Immunohistochemistry (IHC) Protocol (IHC) image
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NCBI/Uniprot data below describe general gene information for CA9. It may not necessarily be applicable to this product.
 NCBI GI #    7327888
 NCBI GeneID    768
 NCBI Accession #    CAB82444.1 [Other Products]
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 UniProt Primary Accession #    Q16790 [Other Products]
 UniProt Secondary Accession #    Q5T4R1 [Other Products]
 UniProt Related Accession #    Q16790 [Other Products]
 Molecular Weight    49,698 Da [Similar Products]
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 NCBI Official Full Name    renal cell carcinoma associated antigen G250
 NCBI Official Synonym Full Names    carbonic anhydrase IX
 NCBI Official Symbol    CA9 [Similar Products]
 NCBI Official Synonym Symbols   
MN; CAIX
[Similar Products]
 NCBI Protein Information    carbonic anhydrase 9; pMW1; CA-IX; P54/58N; membrane antigen MN; carbonic dehydratase; carbonate dehydratase IX; RCC-associated antigen G250; RCC-associated protein G250; renal cell carcinoma-associated antigen G250
 UniProt Protein Name    Carbonic anhydrase 9
 UniProt Synonym Protein Names   
Carbonate dehydratase IX; Carbonic anhydrase IX; CA-IX; CAIX; Membrane antigen MN; P54/58N; Renal cell carcinoma-associated antigen G250; RCC-associated antigen G250; pMW1
 Protein Family    Carbonic anhydrase
 UniProt Gene Name    CA9 [Similar Products]
 UniProt Synonym Gene Names    G250; MN; CA-IX; CAIX [Similar Products]
 UniProt Entry Name    CAH9_HUMAN
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 NCBI Summary for CA9    Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jul 2008]
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 UniProt Comments for CA9    Function: Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. Ref.12

Catalytic activity: H2CO3 = CO2 + H2O.

Cofactor: Zinc.

Enzyme regulation: Inhibited by coumarins, saccharin, sulfonamide derivatives such as acetazolamide (AZA) and Foscarnet (phosphonoformate trisodium salt). Ref.10 Ref.11 Ref.14 Ref.15

Subunit structure: Forms oligomers linked by disulfide bonds. Ref.12 Ref.17

Subcellular location: Nucleus. Nucleus › nucleolus. Cell membrane; Single-pass type I membrane protein. Cell projection › microvillus membrane; Single-pass type I membrane protein. Note: Found on the surface microvilli and in the nucleus, particularly in nucleolus. Ref.8

Tissue specificity: Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.

Induction: By hypoxia. Ref.10 Ref.11 Ref.12 Ref.14 Ref.15

Post-translational modification: Asn-346 bears high-mannose type glycan structures.

Sequence similarities: Belongs to the alpha-carbonic anhydrase family.

Biophysicochemical propertiespH dependence:Optimum pH is 6.5. Ref.17
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 Research Articles on CA9    1. CA-IX appears to be involved in tumor progression and may be diagnostically useful in cases of endocervical adenocarcinoma and its precursors
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-CA9 antibodyPathways associated with anti-CA9 antibody
 Reference Product  PubMed Publications
 HIF1A antibody  >135 publications with CA9 and HIF1A
 HGF antibody  >10 publications with CA9 and HGF
 SRC antibody  >7 publications with CA9 and SRC
 ARNT antibody  >3 publications with CA9 and ARNT
 TP53 antibody  >2 publications with CA9 and TP53
 EP300 antibody  >2 publications with CA9 and EP300
 Products by Pathway  Pathway Diagram
 Cellular Response To Hypoxia Pathway antibodies  Cellular Response To Hypoxia Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 HIF-1-alpha Transcription Factor Network Pathway antibodies  HIF-1-alpha Transcription Factor Network Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Nitrogen Metabolism Pathway antibodies  Nitrogen Metabolism Pathway Diagram
 Nitrogen Metabolism Pathway antibodies  Nitrogen Metabolism Pathway Diagram
 Regulation Of Gene Expression By Hypoxia-inducible Factor Pathway antibodies  Regulation Of Gene Expression By Hypoxia-inducible Factor Pathway Diagram
 Regulation Of Hypoxia-inducible Factor (HIF) By Oxygen Pathway antibodies  Regulation Of Hypoxia-inducible Factor (HIF) By Oxygen Pathway Diagram
 Reversible Hydration Of Carbon Dioxide Pathway antibodies  Reversible Hydration Of Carbon Dioxide Pathway Diagram
Diseases associated with anti-CA9 antibodyOrgans/Tissues associated with anti-CA9 antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >636 publications with CA9 and Neoplasms
 Adenocarcinoma Antibodies  >213 publications with CA9 and Adenocarcinoma
 Kidney Diseases Antibodies  >161 publications with CA9 and Kidney Diseases
 Anoxia Antibodies  >67 publications with CA9 and Anoxia
 Breast Neoplasms Antibodies  >64 publications with CA9 and Breast Neoplasms
 Neoplasms, Experimental Antibodies  >62 publications with CA9 and Neoplasms, Experimental
 Necrosis Antibodies  >46 publications with CA9 and Necrosis
 Neoplasm Invasiveness Antibodies  >41 publications with CA9 and Neoplasm Invasiveness
 Lung Neoplasms Antibodies  >38 publications with CA9 and Lung Neoplasms
 Nervous System Diseases Antibodies  >34 publications with CA9 and Nervous System Diseases
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >167 publications with CA9 and Kidney
 Blood Antibodies  >136 publications with CA9 and Blood
 Lung Antibodies  >48 publications with CA9 and Lung
 Brain Antibodies  >29 publications with CA9 and Brain
 Cervix Antibodies  >29 publications with CA9 and Cervix
 Pancreas Antibodies  >12 publications with CA9 and Pancreas
 Bone Antibodies  >12 publications with CA9 and Bone
 Stomach Antibodies  >10 publications with CA9 and Stomach
 Intestine Antibodies  >9 publications with CA9 and Intestine
 Uterus Antibodies  >8 publications with CA9 and Uterus
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