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anti-S100A8 antibody :: Rat anti-Mouse S100A8 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS602107
Unit / Price
0.1 mg  /  $645 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

S100A8, Monoclonal Antibody

★Popular Item★
 Also Known As   

S100A8 (MRP-8)

 Product Synonym Names    Anti -S100A8 (MRP-8)
 Product Gene Name   

anti-S100A8 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    chromosome: 1; Location: 1q21
 OMIM    123885
 3D Structure    ModBase 3D Structure for P27005
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 Clonality    Monoclonal
 Isotype    IgG2b
 Clone Number    8H150
 Host    Rat
 Species Reactivity    Mouse
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 Specificity    Recognizes mouse S100A8. Species Crossreactivity: Recombinant human S100A8: ~40%. No crossreactivity is observed with recombinant mouse s100A4, recombinant mouse S100A9, recombinant mouse S100A10, recombinant mouse S100A11, recombinant human S100B or recombinant human S100P.
 Purity/Purification    Purified by Protein G affinity chromatography from hybridoma culture supernatant.
 Form/Format    Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
 Concentration    ~0.5 mg/ml (after reconstitution) (lot specific)
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 Grade    Affinity Purified
 Immunogen    Recombinant protein corresponding to Met1-Glu89 (predicted) from mouse from mouse S100A8, expressed in E. coli (P27005).
 Recommeded Secondary Antibodies    MBS612061 IgG, Murine X-Adsorbed (FITC) Pab Gt x Rt
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 Preparation and Storage    Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
 Other Notes    Small volumes of anti-S100A8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-S100A8 antibody

   Mouse S100A8 (also CP-10, calgranulin A and MRP8) is a 10kD member of the S100 family of calcium-binding proteins. The S100A8 protein is 89 amino acids (aa) in length and contains short, sequential modules. There is an N-terminal a-helix, followed by a calcium-binding EF-hand segment, and a C-terminal a-helix. S100A8 will noncovalently heterodimerize with S100A9. In the presence of calcium, this heterodimer will form a heterotetramer. The dimeric complex is both intracellular and extracellular. It binds heparan sulfate and is chemotactic for PMNs and macrophages. Mouse S100A8 is 80% and 57% aa identical to rat and human S100A8, respectively.
 Product Categories/Family for anti-S100A8 antibody    Antibodies; Abs to Binding Proteins
 Applications Tested/Suitable for anti-S100A8 antibody   

ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)

 Application Notes for anti-S100A8 antibody    Western Blot: 2ug/ml using mouse leukocyte
Immunocytochemistry: 8-25 ug/ml using immersion fixed NIH-3T3 mouse embryonic fibroblast cell line

Optimal dilutions to be determined by the researcher.
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 Western Blot (WB) of anti-S100A8 antibody    Western Blot analysis of mouse S100A8 of lysates of mouse leukocyte. PVDF membrane was probed with S0052-17H1 (2 ug/ml followed by IgG (HRP) anti-rat IgG secondary antibody. A specific band was detected for S100A8 at ~10kD (as indicated). This experiment was conducted under reducing conditions and using the buffers listed in the protocol.
anti-S100A8 antibody Western Blot (WB) (WB) image
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 Testing Data(TD) of anti-S100A8 antibody    S100A8 in NIH-3T3 mouse cell line: S100A8 was detected in mmersion fixed NIH-3T3 mouse embryonic fibroblast cell line using 10 ug/mL MBS602107 for 3 hours at RT. Cells were stained (red) and counterstained with DAPI (blue).
anti-S100A8 antibody Testing Data(TD) image
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NCBI/Uniprot data below describe general gene information for S100A8. It may not necessarily be applicable to this product.
 NCBI GI #    47115285
 NCBI GeneID    6279
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 UniProt Primary Accession #    P27005 [Other Products]
 UniProt Secondary Accession #    Q5SY70; Q9UC84; Q9UC92; Q9UCJ0; Q9UCM6; A8K5L3; D3DV37 [Other Products]
 UniProt Related Accession #    P05109 [Other Products]
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 NCBI Official Full Name    S100A8
 NCBI Official Synonym Full Names    S100 calcium binding protein A8
 NCBI Official Symbol    S100A8 [Similar Products]
 NCBI Official Synonym Symbols   
P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG; S100A8
[Similar Products]
 NCBI Protein Information    protein S100-A8; MRP-8; calgranulin A; calgranulin-A; cystic fibrosis antigen; calprotectin L1L subunit; urinary stone protein band A; leukocyte L1 complex light chain; migration inhibitory factor-related protein 8; S100 calcium-binding protein A8 (calgranulin A)
 UniProt Protein Name    Protein S100-A8
 UniProt Synonym Protein Names   
Calgranulin-A; Calprotectin L1L subunit; Cystic fibrosis antigen; CFAG; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8; MRP-8; p8; S100 calcium-binding protein A8; Urinary stone protein band A
 Protein Family    Protein
 UniProt Gene Name    S100A8 [Similar Products]
 UniProt Synonym Gene Names    CAGA; CFAG; MRP8 [Similar Products]
 UniProt Entry Name    S10A8_HUMAN
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 NCBI Summary for S100A8    The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. [provided by RefSeq]
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 UniProt Comments for S100A8    S100A8: S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH- oxidase by facilitating the enzyme complex assembly at the cell membrane, transfering arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF- kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. Belongs to the S-100 family.

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular space; cytoskeleton; plasma membrane; extracellular region; cytosol; nucleus

Molecular Function: arachidonic acid binding; protein binding; RAGE receptor binding; zinc ion binding; microtubule binding; calcium ion binding

Biological Process: caspase activation; neutrophil chemotaxis; chronic inflammatory response; chemokine production; wound healing; cytokine production; response to lipopolysaccharide; positive regulation of peptide secretion; positive regulation of cell growth; leukocyte migration during inflammatory response; activation of NF-kappaB transcription factor; sequestering of zinc ion; response to ethanol; response to zinc ion; defense response to bacterium; innate immune response; autophagy; inflammatory response; defense response to fungus; acute inflammatory response; regulation of cytoskeleton organization and biogenesis; positive regulation of inflammatory response
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 Research Articles on S100A8    1. a single measurement of faecal calprotectin is not sufficiently accurate to identify patients with significant colorectal disease; a normal result can help rule out organic disease among patients with diarrhoea or abdominal pain and/or constipation.
2. Plasma MRP8/14 levels are associated with paediatric obstructive sleep apnea and may reflect increased risk for cardiovascular morbidity
3. S100A8-induced cell death involves BNIP3 and increase of ROS production by mitochondria, subsequently followed by mitochondrial damage and lysosomal activation.
4. Fecal calprotectin dosage shows a good sensitivity but low specificity for the diagnosis of intestinal rejection.
5. Plasma calprotectin has a role in obesity in individuals without type 2 diabetes
6. S100A8/A9 could underpin chronic airway inflammation and airway remodeling in asthma by inducing effector responses of resident and infiltrating airway cells--REVIEW
7. Serum levels of S100A8/A9 are significantly raised in SLE versus pSS patients and healthy controls and can be correlated to a disease activity index.
8. increased expression in sera, synovial tissue and in PBMC of rheumatoid arthritis patients
9. MRP8/14 may be a potentially selective novel biomarker for microcirculatory defects in diabetic nephropathy.
10. expression increases at 24 h (3.0-fold) and 48 h (4.4-fold) after UV skin irradiation
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-S100A8 antibodyDiseases associated with anti-S100A8 antibody
 Reference Product  PubMed Publications
 S100A9 antibody  >477 publications with S100A8 and S100A9
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >231 publications with S100A8 and Inflammation
 Neoplasms Antibodies  >142 publications with S100A8 and Neoplasms
 Fibrosis Antibodies  >87 publications with S100A8 and Fibrosis
 Genetic Diseases, Inborn Antibodies  >77 publications with S100A8 and Genetic Diseases, Inborn
 Carcinoma Antibodies  >61 publications with S100A8 and Carcinoma
 Arthritis, Rheumatoid Antibodies  >47 publications with S100A8 and Arthritis, Rheumatoid
 Cardiovascular Diseases Antibodies  >32 publications with S100A8 and Cardiovascular Diseases
 Intestinal Diseases Antibodies  >32 publications with S100A8 and Intestinal Diseases
 Carcinoma, Squamous Cell Antibodies  >28 publications with S100A8 and Carcinoma, Squamous Cell
 Male Urogenital Diseases Antibodies  >27 publications with S100A8 and Male Urogenital Diseases
Organs/Tissues associated with anti-S100A8 antibody
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >326 publications with S100A8 and Blood
 Skin Antibodies  >86 publications with S100A8 and Skin
 Lung Antibodies  >66 publications with S100A8 and Lung
 Vascular Antibodies  >56 publications with S100A8 and Vascular
 Bone Antibodies  >36 publications with S100A8 and Bone
 Brain Antibodies  >31 publications with S100A8 and Brain
 Muscle Antibodies  >28 publications with S100A8 and Muscle
 Bone Marrow Antibodies  >27 publications with S100A8 and Bone Marrow
 Liver Antibodies  >26 publications with S100A8 and Liver
 Heart Antibodies  >22 publications with S100A8 and Heart
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