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anti-SPARC  antibody :: Rabbit anti-Human SPARC  Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS2544117 anti-SPARC  antibody
Unit / Price
0.06 mL  /  $215 +1 FREE 8GB USB
0.12 mL  /  $330 +1 FREE 8GB USB
0.2 mL  /  $500 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

SPARC , Monoclonal Antibody

 Also Known As   

Rabbit anti Human SPARC  Monoclonal Antibody

 Product Gene Name   

anti-SPARC  antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    Y00755 mRNA
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 Clonality    Monoclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human
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 Immunogen    Recombinant Human SPARC protein
 Buffer    0.2 mum filtered solution in PBS with 5% trehalose
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 Preparation and Storage    This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
 Other Notes    Small volumes of anti-SPARC  antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-SPARC  antibody

   Secreted protein acidic and rich in cysteine (SPARC), also known as Osteonectin (ON), is a member of the SPARC family. SPARC consists of three domains: a EF-hand domain, a follistatin-like domain and a Kazal-like domain, and each of which has independent activity and unique properties. The activity of SPARC is context- and cell-type-dependent, which is highlighted by the fact that SPARC has shown seemingly contradictory effects on tumor progression in both clinical correlative studies and in animal models. The location of SPARC in the nuclear matrix of certain proliferating cells, but only in the cytosol of postmitotic neurons, indicates potential functions of SPARC as a nuclear protein, which might be involved in the regulation of cell cycle progression and mitosis. It functions not only to modulate cell-cell and cell-matrix interactions, but its de-adhesive and growth inhibitory properties in non-transformed cells have led to studies to assess its role in cancer. Its divergent actions reflect the complexity of this protein, because in certain types of cancers, such as melanomas and gliomas, SPARC is associated with a highly aggressive tumor phenotype, while in others, mainly ovarian, neuroblastomas and colorectal cancers, SPARC may function as a tumor suppressor. Recent studies have also demonstrated a role for SPARC in sensitizing therapy-resistant cancers. Notably, SPARC is linked to human obesity.
 Applications Tested/Suitable for anti-SPARC  antibody   

ELISA (EIA)

 Application Notes for anti-SPARC  antibody    ELISA: 0.1-0.2 mug/mL. This antibody can be used at 0.1-0.2 mug/mL with the appropriate secondary reagents to detect Human SPARC. The detection limit for Human SPARC is approximately 0.00975 ng/well.
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NCBI/Uniprot data below describe general gene information for SPARC . It may not necessarily be applicable to this product.
 NCBI GI #    4507171
 NCBI GeneID    6678
 NCBI GenBank Nucleotide #    NM_003118.3 [Other Products]
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 UniProt Secondary Accession #    Q6IBK4; D3DQH9 [Other Products]
 UniProt Related Accession #    P09486 [Other Products]
 Molecular Weight    34,632 Da
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 NCBI Official Full Name    SPARC isoform 1
 NCBI Official Synonym Full Names    secreted protein acidic and cysteine rich
 NCBI Official Symbol    SPARC [Similar Products]
 NCBI Official Synonym Symbols   
ON; OI17; BM-40
[Similar Products]
 NCBI Protein Information    SPARC
 UniProt Protein Name    SPARC
 UniProt Synonym Protein Names   
Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine
 UniProt Gene Name    SPARC [Similar Products]
 UniProt Synonym Gene Names    ON; BM-40; ON [Similar Products]
 UniProt Entry Name    SPRC_HUMAN
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 NCBI Summary for SPARC     This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015]
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 UniProt Comments for SPARC     SPARC: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. Belongs to the SPARC family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q31.3-q32

Cellular Component: cell surface; cytoplasm; extracellular region; extracellular space; mitochondrion; nuclear matrix; plasma membrane; platelet alpha granule membrane

Molecular Function: calcium ion binding; collagen binding; extracellular matrix binding; protein binding

Biological Process: extracellular matrix organization and biogenesis; negative regulation of angiogenesis; negative regulation of endothelial cell proliferation; platelet degranulation; receptor-mediated endocytosis; regulation of cell morphogenesis

Disease: Osteogenesis Imperfecta, Type Xvii
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 Research Articles on SPARC     1. WIN-dependent increase in the level of SPARC plays a critical role in sensitizing osteosarcoma cells to TRAIL action
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-SPARC  antibodyPathways associated with anti-SPARC  antibody
 Reference Product  PubMed Publications
 TGFB1 antibody  >77 publications with SPARC  and TGFB1
 COL1A1 antibody  >44 publications with SPARC  and COL1A1
 COL3A1 antibody  >25 publications with SPARC  and COL3A1
 FN1 antibody  >17 publications with SPARC  and FN1
 TIMP1 antibody  >10 publications with SPARC  and TIMP1
 VEGFA antibody  >10 publications with SPARC  and VEGFA
 VWF antibody  >7 publications with SPARC  and VWF
 ALB antibody  >2 publications with SPARC  and ALB
 PLG antibody  >2 publications with SPARC  and PLG
 Products by Pathway  Pathway Diagram
 Binding And Uptake Of Ligands By Scavenger Receptors Pathway antibodies  Binding And Uptake Of Ligands By Scavenger Receptors Pathway Diagram
 ECM Proteoglycans Pathway antibodies  ECM Proteoglycans Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
 Response To Elevated Platelet Cytosolic Ca2+ Pathway antibodies  Response To Elevated Platelet Cytosolic Ca2+ Pathway Diagram
 Scavenging By Class H Receptors Pathway antibodies  Scavenging By Class H Receptors Pathway Diagram
 Senescence And Autophagy In Cancer Pathway antibodies  Senescence And Autophagy In Cancer Pathway Diagram
 Vesicle-mediated Transport Pathway antibodies  Vesicle-mediated Transport Pathway Diagram
Diseases associated with anti-SPARC  antibodyOrgans/Tissues associated with anti-SPARC  antibody
 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >135 publications with SPARC  and Adenocarcinoma
 Disease Models, Animal Antibodies  >123 publications with SPARC  and Disease Models, Animal
 Inflammation Antibodies  >120 publications with SPARC  and Inflammation
 Fibrosis Antibodies  >111 publications with SPARC  and Fibrosis
 Breast Neoplasms Antibodies  >94 publications with SPARC  and Breast Neoplasms
 Necrosis Antibodies  >71 publications with SPARC  and Necrosis
 Lung Neoplasms Antibodies  >59 publications with SPARC  and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >56 publications with SPARC  and Neoplasms, Experimental
 Cell Transformation, Neoplastic Antibodies  >52 publications with SPARC  and Cell Transformation, Neoplastic
 Colorectal Neoplasms Antibodies  >48 publications with SPARC  and Colorectal Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >1183 publications with SPARC  and Bone
 Connective Tissue Antibodies  >484 publications with SPARC  and Connective Tissue
 Blood Antibodies  >368 publications with SPARC  and Blood
 Vascular Antibodies  >328 publications with SPARC  and Vascular
 Bone Marrow Antibodies  >229 publications with SPARC  and Bone Marrow
 Muscle Antibodies  >211 publications with SPARC  and Muscle
 Embryonic Tissue Antibodies  >184 publications with SPARC  and Embryonic Tissue
 Mouth Antibodies  >176 publications with SPARC  and Mouth
 Brain Antibodies  >168 publications with SPARC  and Brain
 Heart Antibodies  >134 publications with SPARC  and Heart
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