View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Add To Compare List anti-TAQ antibody :: TAQ Monoclonal Antibody Catalog # MBS430075 Unit / Price DISCONTINUEDClick here to view our similar product(s) OR click here to contact our technical support team for suitable replacement. Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name TAQ, Monoclonal Antibody Popular Item Full Product Name TAQ Monoclonal Antibody, clone 8A-31 Product Synonym Names Anti-Taq Monoclonal Antibody; Glycerol-free; Glycerol-free Anti-Taq Monoclonal Antibody (8C1C) Product Gene Name anti-TAQ antibody[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Monoclonal Isotype Mouse IgG1 Clone Number 8A-31 Specificity Reacts with various commercial available Taq DNA polymerases (native or recombinant) Purity/Purification > 97% by SDS-PAGE. Concentration 4 mg/ml in 10mM Tris-HCl (pH 7.0), 50mM KCl. (lot specific) Associated Activities No conversion to the covalently closed circular DNA to the nicked or linear form was observed after incubation of 1 mug of pUC19 with antibodies in final concentration of 1 ng/mul in 20 mul of reaction mixture containing 25 mM Tris-HCl (pH 7.9), 100 mM NaCl, 10 mM MgCl2 after 16 hours at 37 degree C. Reaction Buffer The glycerol-free anti-Taq Monoclonal Antibody reaction buffer is the same buffer used for the thermostable DNA polymerase. Note For each Taq DNA polymerase or Taq from different batch, the antibody titration test must be performed to find the best ratio of antibody to Taq DNA polymerase. Too much antibody added to Taq DNA polymerase could kill the PCR reaction. Protocol 1. Before making any dilution of anti-Taq antibody and Taq DNA polymerase, add 2 ug anti-Taq monoclonal antibody to 1 ug Taq DNA polymerase (50-5,000U).2. Mix gently and incubate at RT for about 5-10 minutes or store at 4 degree C.3. Set up the PCR reaction by following the protocol of a regular thermal cycling condition used for the Taq DNA polymerase. Immunogen Full length Taq DNA polymerase Preparation and Storage Store at -20 degree C. Other Notes Small volumes of anti-TAQ antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for anti-TAQ antibody Description: Anti-Taq DNA Polymerase Monoclonal antibodyMonoclonal anti-Taq antibodies are largely used to block the polymerase activity at low or room temperature, preventing pre-PCR mispriming and primer dimerization. When the temperature is raised, the antibody is quickly inactivated and PCR (or real-time PCR) proceeds. The use of Anti-Taq Monoclonal Antibody significantly improves the specificity of PCR amplification what is especially important for PCR-based, particularly low-copy-number amplifications. The Glycerol-free Monoclonal Anti-Taq Antibody can bind a variety of commercially available Taq DNA polymerases (native or recombinant). The glycerol-free condition eliminates the possible inhibitory effect caused by glycerol in the PCR reaction. Product Categories/Family for anti-TAQ antibody Taq DNA Polymerases Applications Tested/Suitable for anti-TAQ antibody Real-time or regular hot start PCR applicationsPCR diagnostics, genotyping, SNP etc.Long-term storage of PCR kits. Application Notes for anti-TAQ antibody Keep the Taq DNA polymerase from being activated at storage conditions; prevent nonspecific amplification and primer-dimer formation in PCR amplification; preparation of hot-start PCR or qPCR master mixes Testing Data of anti-TAQ antibody TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP