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Mouse anti-Chicken TCR alpha beta (V beta 1) Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS673880
Testing Data
Unit / Price
0.5 mg  /  $410 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

TCR alpha beta (V beta 1), Monoclonal Antibody

 Also Known As   

Mouse Anti-Chicken TCRab/Vb1-FITC

 Product Synonym Names    Mouse Anti-Chicken TCRab/Vb1 - FITC; Mouse Anti-Chicken TCR alpha beta (V beta 1)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Monoclonal
 Isotype    Mouse IgG1 kappa
 Clone Number    TCR2
 Host    Mouse
 Species Reactivity    Chicken
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 Specificity    Chicken TCR alpha beta (V beta 1)
MAb TCR2 precipitates a CD3-associated heterodimer of Mr 90-kDa (two bands of Mr 40-kDa and 50- kDa upon reduction) on chicken peripheral blood T cells.1 Deglycosylation of the heterodimer yields two polypeptides of Mr 34-kDa and 29-kDa from TCR2 precipitates. In the chicken, two distinct subpopulations of alpha beta T cells appear in the thymus subsequent to the appearance of gamma delta T cells. These subpopulations, originally denoted as TCR2 and TCR3,1-3 arise sequentially in the thymus during ontogeny and are now known to represent two distinct V beta families, V beta 1 and V beta 2, respectively.4 The TCR2 MAb reacts with approximately 40% of thymocytes, 40-50% of blood mononuclear cells and 40% of splenocytes in the chicken. Two-color immunofluorescence has revealed that most of the TCR2+ thymocytes express both CD4 and CD8 antigens. The TCR2+ cells in blood were found to express either CD4 (74+/-2%) or CD8 (26+/-4%). TCR2+ cells in the spleen also express either CD4 (37+/-1%) or CD8 (64+/-4%). Surprisingly, a relatively large subpopulation of CD8+ cells in the spleen are negative for TCR2.1-3 This observation led to the demonstration that 71+/-6% of the TCR1(gamma delta)+ cells in the spleen express the CD8 marker.1-4
 Form/Format    Fluorescein (FITC) Conjugate
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 Characterization    To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested to conform with characteristics of a standard reference reagent using immunofluorescence staining and analysis on a Becton Dickinson FACScanTM flow cytometer.
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 Preparation and Storage    The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
 Other Notes    Small volumes of anti-TCR alpha beta (V beta 1) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-TCR alpha beta (V beta 1) antibody   

Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation

 Application Notes for anti-TCR alpha beta (V beta 1) antibody    Research Application(s): In ovo suppression of the development of TCR-V beta 1 cells

Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
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 Testing Data of anti-TCR alpha beta (V beta 1) antibody    anti-TCR alpha beta (V beta 1) antibody Testing Data image
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Product References and Citations for anti-TCR alpha beta (V beta 1) antibody

   1. Chen, C.H., J. Cihak, U. Losch, and M.D. Cooper. 1988. Eur. J. Immunol. 18:539.
2. Chen, C.H., J.T. Sowder, J.M. Lahti, J. Cihak, U. Losch, and M.D. Cooper. 1989. Proc. Natl. Acad. Sci. (USA):86:2351
3. Char, D., P. Sanchez, C.H. Chen, R.P. Bucy, and M.D. Cooper. 1990. J. Immunol. 145:3547.
4. Chen, C.H., T.W.F. Gobel, T. Kubota, and M.D. Cooper. 1993. 1994. Poultry Science 73:1012.
5. Sowder, J.T., C.H. Chen, L.L. Ager, J. Cihak, U. Losch, and M.D. Cooper. 1988b. Fed. Proc. 2:874.
6. Cihak, J., U. Losch, G. Hoffman-Hezer, C.H. CHen, M.D. Cooper, and H.W.L. Ziegler-Heitbrock. 1993. Scand. J. Immunol. 38:123.
7. Chen, C.H. 1996. Personal communication.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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