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anti-TNF antibody :: Rat anti-Mouse TNF- alpha Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS670093 anti-TNF antibody
Unit / Price
0.5 mg  /  $295 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

TNF- alpha, Monoclonal Antibody

 Also Known As   

Rat Anti-Mouse TNF-a-BIOT

 Product Synonym Names    Rat Anti-Mouse TNF-a - Biotin; Rat Anti-Mouse TNF- alpha
 Product Gene Name   

anti-TNF antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 6; NC_000006.11 (31543350..31546112). Location: 6p21.3
 OMIM    191160
 3D Structure    ModBase 3D Structure for P01375
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 Clonality    Monoclonal
 Isotype    Rat IgG1 kappa
 Clone Number    MP6-XT3
 Host    Rat
 Species Reactivity    Mouse
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 Specificity    Mouse Tumor Necrosis Factor- alpha (TNF- alpha)1,2 This is a neutralizing antibody.
 Form/Format    Biotin (BIOT) Conjugate
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 Immunogen    E. coli-expressed recombinant mouse TNF- alpha
 Characterization    To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.
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 Preparation and Storage    The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg labeled antibody in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
 Other Notes    Small volumes of anti-TNF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-TNF antibody   

ELISA; Western Blot

 Application Notes for anti-TNF antibody    Research Application(s): ELISA Detection: MP6-XT3 monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying mouse TNF- alpha protein levels.
Western Blotting: The purified MP6-XT3 antibody is also useful for Western blotting applications. For Western blotting, a concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of <= 100 ng/lane of recombinant mouse TNF- alpha under reducing conditions.
Neutralization: The LE/AF form of MP6-XT3 can be used for neutralizing mouse TNF- alpha bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control.
Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.
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NCBI/Uniprot data below describe general gene information for TNF. It may not necessarily be applicable to this product.
 NCBI GI #    37210
 NCBI GeneID    7124
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 UniProt Primary Accession #    P01375 [Other Products]
 UniProt Secondary Accession #    O43647; Q9P1Q2; Q9UIV3 [Other Products]
 UniProt Related Accession #    P01375; Q5RT83; Q5STB3; Q9UBM5 [Other Products]
 Molecular Weight    25,644 Da [Similar Products]
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 NCBI Official Full Name    TNF-alpha
 NCBI Official Synonym Full Names    tumor necrosis factor
 NCBI Official Symbol    TNF [Similar Products]
 NCBI Official Synonym Symbols   
DIF; TNFA; TNFSF2; TNF-alpha
[Similar Products]
 NCBI Protein Information    tumor necrosis factor; TNF-a; cachectin; APC1 protein; OTTHUMP00000029281; TNF, monocyte-derived; TNF, macrophage-derived; TNF superfamily, member 2; tumor necrosis factor alpha; tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2
 UniProt Protein Name    Tumor necrosis factor
 UniProt Synonym Protein Names   
Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2
 Protein Family    Tumor necrosis factor
 UniProt Gene Name    TNF [Similar Products]
 UniProt Synonym Gene Names    TNFA; TNFSF2 [Similar Products]
 UniProt Entry Name    TNFA_HUMAN
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 NCBI Summary for TNF    This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq]
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 UniProt Comments for TNF    Function: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

Subunit structure: Homotrimer.

Subcellular location: Cell membrane; Single-pass type II membrane protein. Tumor necrosis factor, soluble form: Secreted.

Post-translational modification: The soluble form derives from the membrane form by proteolytic processing.The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. Ref.18 Ref.19O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. Ref.15

Polymorphism: Genetic variations in TNF influence susceptibility to hepatitis B virus (HBV) infection [

MIM:610424].

Involvement in disease: Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [

MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).

Sequence similarities: Belongs to the tumor necrosis factor family.

Sequence caution: The sequence AAF71992.1 differs from that shown. Reason: Frameshift at positions 91 and 157. The sequence CAA75070.1 differs from that shown. Reason: Erroneous gene model prediction.
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Product References and Citations for anti-TNF antibody

   1. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Immunol. Rev. 127:5.
2. Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current
Protocols in Immunology (J. Coligamn, A. Kruisbeek, D. Margulies, E. Shevach, W. Strober, eds.). John Wiley and Sons, New York. Unit 6.20.
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 Research Articles on TNF    1. Data show that the number of TNF-alpha and CD68 positive cells in HIZ was significantly higher than that in the annulus fibrosus around HIZ and in the control.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-TNF antibodyPathways associated with anti-TNF antibody
 Reference Product  PubMed Publications
 TRAF2 antibody  >818 publications with TNF and TRAF2
 TNFRSF1A antibody  >263 publications with TNF and TNFRSF1A
 IFNG antibody  >78 publications with TNF and IFNG
 ADAM17 antibody  >75 publications with TNF and ADAM17
 Products by Pathway  Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
Diseases associated with anti-TNF antibodyOrgans/Tissues associated with anti-TNF antibody
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >21448 publications with TNF and Inflammation
 Infection Antibodies  >12805 publications with TNF and Infection
 Neoplasms Antibodies  >11734 publications with TNF and Neoplasms
 Cardiovascular Diseases Antibodies  >6102 publications with TNF and Cardiovascular Diseases
 Lung Diseases Antibodies  >4957 publications with TNF and Lung Diseases
 Arthritis, Rheumatoid Antibodies  >4921 publications with TNF and Arthritis, Rheumatoid
 Sepsis Antibodies  >4127 publications with TNF and Sepsis
 Skin Diseases Antibodies  >4065 publications with TNF and Skin Diseases
 Liver Diseases Antibodies  >3609 publications with TNF and Liver Diseases
 Leukemia Antibodies  >2492 publications with TNF and Leukemia
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >39826 publications with TNF and Blood
 Lung Antibodies  >7168 publications with TNF and Lung
 Brain Antibodies  >4726 publications with TNF and Brain
 Heart Antibodies  >3603 publications with TNF and Heart
 Intestine Antibodies  >2551 publications with TNF and Intestine
 Connective Tissue Antibodies  >1629 publications with TNF and Connective Tissue
 Lymph Node Antibodies  >1543 publications with TNF and Lymph Node
 Placenta Antibodies  >650 publications with TNF and Placenta
 Prostate Antibodies  >593 publications with TNF and Prostate
 Ovary Antibodies  >449 publications with TNF and Ovary
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