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anti-TNF-alpha antibody :: Mouse anti-Human TNF-alpha Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS2534301
Testing Data
Unit / Price
25 Tests  /  $195 +1 FREE 8GB USB
100 Tests  /  $425 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

TNF-alpha, Monoclonal Antibody

 Also Known As   

Anti-Human TNF-alpha Antibody (PE conjugated)

 Product Gene Name   

anti-TNF-alpha antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    191160
 3D Structure    ModBase 3D Structure for P01375
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 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    MAb11
 Host    Mouse
 Species Reactivity    Human
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 Concentration    0.2mg/ml (lot specific)
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 Conjugation    PE
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 Preparation and Storage    Keep as concentrated solution. Store at 4 degree C and protected from prolonged exposure to light. Do not freeze.
 Other Notes    Small volumes of anti-TNF-alpha antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-TNF-alpha antibody

   TNF-alpha is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-alpha. Monomeric human TNF-alpha is a 157 amino acid protein (non-glycosylated) with a reported molecular weight of 17 kD. TNF-alpha forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-alpha has also been described. TNF-alpha binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.
 Application Notes for anti-TNF-alpha antibody    Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis (The amount of the reagent is suggested to be used <= 0.5 ug /106 cells in 100 ul). Since applications vary, the appropriate dilutions must be determined for individual use.
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 Testing Data of anti-TNF-alpha antibody    PMA/Ionomycin-stimulated human PBMCs were stained with PE/CY5 anti-human CD3 and PE anti-human TNF-alpha
anti-TNF-alpha antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for TNF-alpha. It may not necessarily be applicable to this product.
 NCBI GI #    37210
 NCBI GeneID    7124
 NCBI Accession #    CAA26669.1 [Other Products]
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 UniProt Primary Accession #    P01375 [Other Products]
 UniProt Secondary Accession #    O43647; Q9P1Q2; Q9UIV3 [Other Products]
 UniProt Related Accession #    P01375 [Other Products]
 Molecular Weight    25,644 Da
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 NCBI Official Full Name    TNF-alpha
 NCBI Official Synonym Full Names    tumor necrosis factor
 NCBI Official Symbol    TNF [Similar Products]
 NCBI Official Synonym Symbols   
DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha
[Similar Products]
 NCBI Protein Information    tumor necrosis factor
 UniProt Protein Name    Tumor necrosis factor
 UniProt Synonym Protein Names   
Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
 UniProt Gene Name    TNF [Similar Products]
 UniProt Synonym Gene Names    TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2 [Similar Products]
 UniProt Entry Name    TNFA_HUMAN
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 NCBI Summary for TNF-alpha    This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]
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 UniProt Comments for TNF-alpha    TNF-a: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Homotrimer. Interacts with SPPL2B. Belongs to the tumor necrosis factor family.

Protein type: Cytokine; Motility/polarity/chemotaxis; Apoptosis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: cell surface; external side of plasma membrane; extracellular region; extracellular space; integral to plasma membrane; lipid raft; phagocytic cup; plasma membrane; recycling endosome

Molecular Function: cytokine activity; identical protein binding; protease binding; protein binding; tumor necrosis factor receptor binding

Biological Process: activation of MAPK activity; activation of MAPKKK activity; activation of NF-kappaB transcription factor; caspase activation; cell surface receptor linked signal transduction; chronic inflammatory response to antigenic stimulus; cortical actin cytoskeleton organization and biogenesis; defense response to Gram-positive bacterium; DNA damage response, signal transduction resulting in induction of apoptosis; embryonic gut development; extracellular matrix organization and biogenesis; glucose metabolic process; humoral immune response; I-kappaB kinase/NF-kappaB cascade; induction of apoptosis via death domain receptors; inflammatory response; JNK cascade; leukocyte tethering or rolling; lipopolysaccharide-mediated signaling pathway; MAPKKK cascade; negative regulation of cytokine secretion during immune response; negative regulation of fat cell differentiation; negative regulation of glucose import; negative regulation of interleukin-6 production; negative regulation of lipid catabolic process; negative regulation of myoblast differentiation; negative regulation of osteoblast differentiation; negative regulation of protein complex disassembly; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of viral genome replication; osteoclast differentiation; positive regulation of apoptosis; positive regulation of caspase activity; positive regulation of cell adhesion; positive regulation of chemokine biosynthetic process; positive regulation of chemokine production; positive regulation of chronic inflammatory response to antigenic stimulus; positive regulation of cytokine production; positive regulation of cytokine secretion; positive regulation of fever; positive regulation of hair follicle development; positive regulation of heterotypic cell-cell adhesion; positive regulation of humoral immune response mediated by circulating immunoglobulin; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interferon-gamma production; positive regulation of interleukin-6 production; positive regulation of interleukin-8 biosynthetic process; positive regulation of interleukin-8 production; positive regulation of JNK activity; positive regulation of MAP kinase activity; positive regulation of membrane protein ectodomain proteolysis; positive regulation of NF-kappaB import into nucleus; positive regulation of NFAT protein import into nucleus; positive regulation of nitric oxide biosynthetic process; positive regulation of osteoclast differentiation; positive regulation of peptidyl-serine phosphorylation; positive regulation of phagocytosis; positive regulation of programmed cell death; positive regulation of protein amino acid phosphorylation; positive regulation of protein complex assembly; positive regulation of protein complex disassembly; positive regulation of protein kinase activity; positive regulation of protein kinase B signaling cascade; positive regulation of protein transport; positive regulation of smooth muscle cell proliferation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of translational initiation by iron; protein import into nucleus, translocation; protein kinase B signaling cascade; receptor biosynthetic process; regulation of I-kappaB kinase/NF-kappaB cascade; regulation of immunoglobulin secretion; regulation of insulin secretion; response to glucocorticoid stimulus; response to salt stress; response to virus; sequestering of triacylglycerol; transformed cell apoptosis; tumor necrosis factor-mediated signaling pathway

Disease: Asthma, Susceptibility To; Malaria, Susceptibility To; Migraine With Or Without Aura, Susceptibility To, 1
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 Research Articles on TNF-alpha    1. Data suggest that promoter activity and expression of miRNA let-7 (microRNA lethal-7) gene cluster in skeletal muscle satellite cells are up-regulated in response to glucose, TNFa (tumor necrosis factor-alpha), and caffeine (but not insulin).
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-TNF-alpha antibodyPathways associated with anti-TNF-alpha antibody
 Reference Product  PubMed Publications
 ICAM1 antibody  >3527 publications with TNF-alpha and ICAM1
 FOXP3 antibody  >728 publications with TNF-alpha and FOXP3
 ITGAM antibody  >699 publications with TNF-alpha and ITGAM
 CD83 antibody  >559 publications with TNF-alpha and CD83
 TNFRSF1A antibody  >464 publications with TNF-alpha and TNFRSF1A
 MYC antibody  >438 publications with TNF-alpha and MYC
 MMP9 antibody  >313 publications with TNF-alpha and MMP9
 IFNG antibody  >193 publications with TNF-alpha and IFNG
 RELB antibody  >170 publications with TNF-alpha and RELB
 IKBKG antibody  >71 publications with TNF-alpha and IKBKG
 Products by Pathway  Pathway Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 AhR Pathway antibodies  AhR Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
Diseases associated with anti-TNF-alpha antibodyOrgans/Tissues associated with anti-TNF-alpha antibody
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >97241 publications with TNF-alpha and Necrosis
 Inflammation Antibodies  >36893 publications with TNF-alpha and Inflammation
 Lung Diseases Antibodies  >7397 publications with TNF-alpha and Lung Diseases
 Liver Diseases Antibodies  >5693 publications with TNF-alpha and Liver Diseases
 Insulin Resistance Antibodies  >2808 publications with TNF-alpha and Insulin Resistance
 Fibrosis Antibodies  >2778 publications with TNF-alpha and Fibrosis
 Colitis Antibodies  >2753 publications with TNF-alpha and Colitis
 Atherosclerosis Antibodies  >2663 publications with TNF-alpha and Atherosclerosis
 Pain Antibodies  >2572 publications with TNF-alpha and Pain
 Reperfusion Injury Antibodies  >2397 publications with TNF-alpha and Reperfusion Injury
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >55300 publications with TNF-alpha and Blood
 Lung Antibodies  >10508 publications with TNF-alpha and Lung
 Brain Antibodies  >7864 publications with TNF-alpha and Brain
 Heart Antibodies  >5538 publications with TNF-alpha and Heart
 Intestine Antibodies  >4054 publications with TNF-alpha and Intestine
 Connective Tissue Antibodies  >2482 publications with TNF-alpha and Connective Tissue
 Lymph Node Antibodies  >2072 publications with TNF-alpha and Lymph Node
 Placenta Antibodies  >916 publications with TNF-alpha and Placenta
 Prostate Antibodies  >872 publications with TNF-alpha and Prostate
 Ovary Antibodies  >585 publications with TNF-alpha and Ovary
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