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anti-TRIM28 antibody :: Mouse anti-Human TRIM28 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS120011
Unit / Price
0.1 mg  /  $345 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

TRIM28, Monoclonal Antibody

★Popular Item★
 Also Known As   

Mouse monoclonal antibody Anti-Human TRIM28

 Product Synonym Names    tripartite motif-containing 28 (TRIM28); KAP1; TF1B; RNF96; TIF1B
 Product Gene Name   

anti-TRIM28 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    601742
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 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    TRI5I093
 Host    Mouse
 Species Reactivity    Human
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 Concentration    100 ug/ml (1.0 ml) (lot specific)
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 Preparation    This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
 Sterility    Filtered through a 0.22 um membrane.
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 Other Notes    Small volumes of anti-TRIM28 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-TRIM28 antibody

   The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [NCBI Entrez Gene Summary]
 Applications Tested/Suitable for anti-TRIM28 antibody   

Dot Blot (DB), Immunocytochemistry (ICC), Western Blot (WB)

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 Quality Control of anti-TRIM28 antibody    Left: Western blot analysis of immunized recombinant protein, using anti-TRIM28 monoclonal antibody.
anti-TRIM28 antibody Quality Control image
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 Western Blot (WB) of anti-TRIM28 antibody    Detection of TRIM28 by Western blot.

Samples: Whole cell lysate from human HEK293 (H, 25 ug) and mouse NIH3T3 (M, 25 ug) cells. [Lot No. TRI5I093-2]

Predicted molecular weight: 88 kDa
anti-TRIM28 antibody Western Blot (WB) (WB) image
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 Immunocytochemistry (ICC) of anti-TRIM28 antibody    Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-TRIM28 mAb. [Lot No. TRI5I093-2]
anti-TRIM28 antibody Immunocytochemistry (ICC) (ICC) image
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NCBI/Uniprot data below describe general gene information for TRIM28. It may not necessarily be applicable to this product.
 NCBI GI #    14971416
 NCBI GeneID    10155
 NCBI Accession #    NP_005753.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_005762 [Other Products]
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 UniProt Secondary Accession #    O00677; Q7Z632; Q93040; Q96IM1 [Other Products]
 UniProt Related Accession #    Q13263 [Other Products]
 Molecular Weight    79,474 Da
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 NCBI Official Full Name    Homo sapiens tripartite motif containing 28 (TRIM28), mRNA
 NCBI Official Synonym Full Names    tripartite motif containing 28
 NCBI Official Symbol    TRIM28 [Similar Products]
 NCBI Official Synonym Symbols   
KAP1; TF1B; RNF96; TIF1B; PPP1R157
[Similar Products]
 NCBI Protein Information    transcription intermediary factor 1-beta; E3 SUMO-protein ligase TRIM28; KAP-1; KRAB [Kruppel-associated box domain]-associated protein 1; KRAB-associated protein 1; KRAB-interacting protein 1; KRIP-1; RING finger protein 96; TIF1-beta; nuclear corepressor KAP-1; protein phosphatase 1, regulatory subunit 157; transcriptional intermediary factor 1-beta; tripartite motif-containing 28; tripartite motif-containing protein 28
 UniProt Protein Name    Transcription intermediary factor 1-beta
 UniProt Synonym Protein Names   
E3 SUMO-protein ligase TRIM28 (EC:6.3.2.-); KRAB-associated protein 1; KAP-1; KRAB-interacting protein 1; KRIP-1; Nuclear corepressor KAP-1; RING finger protein 96; Tripartite motif-containing protein 28
 Protein Family    Transcription intermediary factor
 UniProt Gene Name    TRIM28 [Similar Products]
 UniProt Entry Name    TIF1B_HUMAN
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 NCBI Summary for TRIM28    The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]
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 UniProt Comments for TRIM28    TIF1B: an atypical protein kinase that functions as a transcriptional coactivator. Mediates transcriptional regulation by interaction with the Kruppel-associated box (KRAB) repression domain found in many transcription factors. Binds to the negative regulatory domain of c-Myb and negatively regulates the c-Myb-dependent trans-activation. Localizes to the nucleus and is thought to associate with specific chromatin regions. Phosphorylated in an ATM-dependent manner following double-strand breaks. Its phosphorylation in response to DNA damage leads to chromatin relaxation. A member of the tripartite motif family. The tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

Protein type: Kinase, protein; Transcription, coactivator/corepressor; Ubiquitin ligase; Protein kinase, atypical; Ubiquitin conjugating system; EC 6.3.2.-; Ligase; Nuclear receptor co-regulator; ATYPICAL group; TIF1 family

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: nucleoplasm; nuclear heterochromatin; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; sequence-specific DNA binding; ubiquitin protein ligase binding; transcription coactivator activity; ubiquitin-protein ligase activity; transcription corepressor activity; transcription factor activity; ligase activity; protein kinase activity

Biological Process: transcription initiation from RNA polymerase II promoter; convergent extension involved in axis elongation; positive regulation of DNA repair; protein amino acid autophosphorylation; positive regulation of transcription, DNA-dependent; protein ubiquitination; negative regulation of transcription from RNA polymerase II promoter; DNA repair; protein oligomerization; protein sumoylation; positive regulation of transcription factor import into nucleus; epithelial to mesenchymal transition; innate immune response; gene expression; negative regulation of transcription, DNA-dependent; DNA methylation during embryonic development; embryo implantation
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 Research Articles on TRIM28    1. MAGE proteins bind to KAP1, a gene repressor and ubiquitin E3 ligase which also binds KRAB domain containing zinc finger transcription factors (KZNFs), and MAGE expression may affect KZNF mediated gene regulation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-TRIM28 antibodyPathways associated with anti-TRIM28 antibody
 Reference Product  PubMed Publications
 HDAC1 antibody  >16 publications with TRIM28 and HDAC1
 MDM2 antibody  >6 publications with TRIM28 and MDM2
 ZNF10 antibody  >6 publications with TRIM28 and ZNF10
 E2F1 antibody  >4 publications with TRIM28 and E2F1
 CBX3 antibody  >3 publications with TRIM28 and CBX3
 UBC antibody  >1 publications with TRIM28 and UBC
 CBX1 antibody  >1 publications with TRIM28 and CBX1
 CBX5 antibody  >1 publications with TRIM28 and CBX5
 Products by Pathway  Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
 Corticotropin-releasing Hormone Pathway antibodies  Corticotropin-releasing Hormone Pathway Diagram
 E2F Transcription Factor Network Pathway antibodies  E2F Transcription Factor Network Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 P53 Pathway antibodies  P53 Pathway Diagram
Diseases associated with anti-TRIM28 antibodyOrgans/Tissues associated with anti-TRIM28 antibody
 Disease Name  Pubmed Publications
 Neoplasm Metastasis Antibodies  >5 publications with TRIM28 and Neoplasm Metastasis
 Disease Models, Animal Antibodies  >5 publications with TRIM28 and Disease Models, Animal
 Liver Diseases Antibodies  >3 publications with TRIM28 and Liver Diseases
 Liver Neoplasms Antibodies  >3 publications with TRIM28 and Liver Neoplasms
 Lung Diseases Antibodies  >3 publications with TRIM28 and Lung Diseases
 Anemia Antibodies  >3 publications with TRIM28 and Anemia
 Inflammation Antibodies  >3 publications with TRIM28 and Inflammation
 Carcinoma, Hepatocellular Antibodies  >2 publications with TRIM28 and Carcinoma, Hepatocellular
 Vascular Diseases Antibodies  >2 publications with TRIM28 and Vascular Diseases
 Cardiovascular Diseases Antibodies  >2 publications with TRIM28 and Cardiovascular Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >17 publications with TRIM28 and Blood
 Embryonic Tissue Antibodies  >7 publications with TRIM28 and Embryonic Tissue
 Liver Antibodies  >6 publications with TRIM28 and Liver
 Testis Antibodies  >6 publications with TRIM28 and Testis
 Brain Antibodies  >6 publications with TRIM28 and Brain
 Kidney Antibodies  >5 publications with TRIM28 and Kidney
 Lung Antibodies  >4 publications with TRIM28 and Lung
 Bone Antibodies  >4 publications with TRIM28 and Bone
 Bone Marrow Antibodies  >3 publications with TRIM28 and Bone Marrow
 Muscle Antibodies  >3 publications with TRIM28 and Muscle
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