Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Antibody rightarrow TWIST rightarrow Monoclonal TWIST  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Annexin V (ANN V) ELISA Kit
 • LOX Recombinant Protein
 • Interleukin 1beta, IL-1beta ELISA Kit
 • neural precursor cell expressed, developmentally down-regula... ELISA Kit
 • Galanthus nivalis Lectin
 • haptoglobin (HP) ELISA Kit
 • Epidermal Growth Factor (EGF) Recombinant Protein
 • C3a ELISA Kit
 • ENO3 cDNA Clone
 • Indole-3 butyric acid (IBA) Biochemical
 • transcription factor EB (TFEB) Antibody
 • Inhibin Beta E (INHbE) Antibody
 • GYS1 Antibody
 • hepatitis B virus surface antigen, HBsAg ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

anti-TWIST antibody :: Mouse anti-Human TWIST Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS120163
Unit / Price
0.1 mg  /  $345 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

TWIST, Monoclonal Antibody

★Popular Item★
 Also Known As   

Mouse monoclonal antibody Anti-Human TWIST

 Product Synonym Names    Homo sapiens twist homolog 1 (acrocephalosyndactyly 3; Saethre-Chotzen syndrome) (Drosophila) (TWIST1); SCS;ACS3; BPES2; BPES3;TWIST1
 Product Gene Name   

anti-TWIST antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 OMIM    101400
Section Bar
 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    Twist2C1a
 Host    Mouse
 Species Reactivity    Human
Section Bar
 Concentration    100 ug/ml (1.0 ml) (lot specific)
Section Bar
 Preparation    This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
 Sterility    Filtered through a 0.22 um membrane.
Section Bar
 Other Notes    Small volumes of anti-TWIST antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 

Related Product Information for anti-TWIST antibody

   Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome. [NCBI Entrez Gene Summary]
 Applications Tested/Suitable for anti-TWIST antibody   

Dot Blot (DB), Immunocytochemistry (ICC), Western Blot (WB), Flow Cytometry (FC/FACS)

Section Bar
 Quality Control of anti-TWIST antibody    Western blot analysis of immunized recombinant protein, using anti-TWIST monoclonal antibody.
anti-TWIST antibody Quality Control image
Section Bar
 Quality Control #2 of anti-TWIST antibody    Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.
anti-TWIST antibody Quality Control #2 image
Section Bar
 Western Blot (WB) of anti-TWIST antibody    Detection of TWIST by Western blot.

Samples: Whole cell lysate from human A2058 (H, 25 ug), mouse NIH3T3 (M, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. Twist2C1a-1]

Predicted molecular weight: 20 kDa
anti-TWIST antibody Western Blot (WB) (WB) image
Section Bar
 Flow Cytometry (FC/FACS) of anti-TWIST antibody    HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-TWIST mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. Twist2C1a-1]
anti-TWIST antibody Flow Cytometry (FC/FACS) (FC/FACS) image
Section Bar
 Immunocytochemistry (ICC) of anti-TWIST antibody    Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-TWIST mAb. [Lot No. Twist2C1a-1]
anti-TWIST antibody Immunocytochemistry (ICC) (ICC) image
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for TWIST. It may not necessarily be applicable to this product.
 NCBI GI #    68160957
 NCBI GeneID    7291
 NCBI Accession #    NP_000465.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000474 [Other Products]
Section Bar
 UniProt Secondary Accession #    Q92487; Q99804; A4D128 [Other Products]
 UniProt Related Accession #    Q15672 [Other Products]
 Molecular Weight    20,954 Da
Table BarTOPTable Bar
 NCBI Official Full Name    Homo sapiens twist family bHLH transcription factor 1 (TWIST1), mRNA
 NCBI Official Synonym Full Names    twist family bHLH transcription factor 1
 NCBI Official Symbol    TWIST1 [Similar Products]
 NCBI Official Synonym Symbols   
CRS; CSO; SCS; ACS3; CRS1; BPES2; BPES3; TWIST; bHLHa38
[Similar Products]
 NCBI Protein Information    twist-related protein 1; B-HLH DNA binding protein; H-twist; TWIST homolog of drosophila; class A basic helix-loop-helix protein 38; twist basic helix-loop-helix transcription factor 1; twist homolog 1
 UniProt Protein Name    Twist-related protein 1
 UniProt Synonym Protein Names   
Class A basic helix-loop-helix protein 38; bHLHa38; H-twist
 Protein Family    Twist-related protein
 UniProt Gene Name    TWIST1 [Similar Products]
 UniProt Entry Name    TWST1_HUMAN
Section Bar
 NCBI Summary for TWIST    Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for TWIST    TWIST1: Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. Efficient DNA binding requires dimerization with another bHLH protein. Homodimer or heterodimer with E proteins such as TCF3. ID1 binds preferentially to TCF3 but does not interact efficiently with TWIST1 so ID1 levels control the amount of TCF3 available to dimerize with TWIST1 and thus determine the type of dimer formed. Subset of mesodermal cells.

Protein type: Transcription regulation; Cell development/differentiation; Inhibitor; DNA-binding

Chromosomal Location of Human Ortholog: 7p21.2

Cellular Component: nucleus

Molecular Function: protein domain specific binding; protein binding; protein homodimerization activity; protein heterodimerization activity; bHLH transcription factor binding; transcription factor binding

Biological Process: embryonic forelimb morphogenesis; transcription from RNA polymerase II promoter; negative regulation of skeletal muscle development; muscle development; neuron migration; rhythmic process; negative regulation of transcription from RNA polymerase II promoter; palate development; embryonic hindlimb morphogenesis; negative regulation of histone phosphorylation; negative regulation of histone acetylation; odontogenesis; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of osteoblast differentiation; ossification; in utero embryonic development; negative regulation of transcription factor activity; outer ear morphogenesis; negative regulation of tumor necrosis factor production; embryonic cranial skeleton morphogenesis; positive regulation of tumor necrosis factor production; positive regulation of fatty acid beta-oxidation; osteoblast differentiation; positive regulation of angiogenesis; neural tube closure; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; negative regulation of transcription, DNA-dependent; regulation of bone mineralization; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; negative regulation of phosphoinositide 3-kinase cascade

Disease: Robinow-sorauf Syndrome; Craniosynostosis 1; Saethre-chotzen Syndrome
Table BarTOPTable Bar
 Research Articles on TWIST    1. Id4 has a role in suppressing MMP2-mediated invasion of glioblastoma-derived cells by direct inactivation of Twist1 function
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with anti-TWIST antibodyPathways associated with anti-TWIST antibody
 Reference Product  PubMed Publications
 RUNX2 antibody  >45 publications with TWIST and RUNX2
 MSX2 antibody  >24 publications with TWIST and MSX2
 CDH2 antibody  >17 publications with TWIST and CDH2
 TCF3 antibody  >15 publications with TWIST and TCF3
 RELA antibody  >6 publications with TWIST and RELA
 SMAD4 antibody  >4 publications with TWIST and SMAD4
 HDAC2 antibody  >1 publications with TWIST and HDAC2
 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 HIF-2-alpha Transcription Factor Network Pathway antibodies  HIF-2-alpha Transcription Factor Network Pathway Diagram
 Neural Crest Differentiation Pathway antibodies  Neural Crest Differentiation Pathway Diagram
 Notch-mediated HES/HEY Network Pathway antibodies  Notch-mediated HES/HEY Network Pathway Diagram
 Proteoglycans In Cancer Pathway antibodies  Proteoglycans In Cancer Pathway Diagram
 Proteoglycans In Cancer Pathway antibodies  Proteoglycans In Cancer Pathway Diagram
Diseases associated with anti-TWIST antibodyOrgans/Tissues associated with anti-TWIST antibody
 Disease Name  Pubmed Publications
 Neoplasm Metastasis Antibodies  >171 publications with TWIST and Neoplasm Metastasis
 Congenital Abnormalities Antibodies  >150 publications with TWIST and Congenital Abnormalities
 Breast Neoplasms Antibodies  >130 publications with TWIST and Breast Neoplasms
 Neoplasm Invasiveness Antibodies  >129 publications with TWIST and Neoplasm Invasiveness
 Craniosynostoses Antibodies  >126 publications with TWIST and Craniosynostoses
 Adenocarcinoma Antibodies  >103 publications with TWIST and Adenocarcinoma
 Acrocephalosyndactylia Antibodies  >76 publications with TWIST and Acrocephalosyndactylia
 Lung Diseases Antibodies  >60 publications with TWIST and Lung Diseases
 Lung Neoplasms Antibodies  >60 publications with TWIST and Lung Neoplasms
 Cell Transformation, Neoplastic Antibodies  >42 publications with TWIST and Cell Transformation, Neoplastic
 Organ/Tissue Name  Pubmed Publications
 Ear Antibodies  >12 publications with TWIST and Ear
 Eye Antibodies  >9 publications with TWIST and Eye
 Mixed Antibodies  >8 publications with TWIST and Mixed
 Uterus Antibodies  >6 publications with TWIST and Uterus
 Placenta Antibodies  >4 publications with TWIST and Placenta
 Ovary Antibodies  >3 publications with TWIST and Ovary
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions