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anti-TTF-1 antibody :: Rabbit Thyroid Transcription Factor-1 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS303162
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 Product Name   

Thyroid Transcription Factor-1 (TTF-1), Monoclonal Antibody

★Popular Item★
 Also Known As   

Rabbit Anti-Human Thyroid Transcription Factor-1 (TTF-1) Monoclonal Antibody (Clone SP141)

 Product Gene Name   

anti-TTF-1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Monoclonal
 Isotype    IgG
 Clone Number    SP141
 Host    Rabbit
 Species Reactivity    Human
Predicted Reactivity: Mouse, Rat
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 Form/Format    0.1 ml rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.
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 Immunogen    Synthetic peptide derived from the internal region of human TTF-1 protein.
 Control Tissue    Lung, Thyroid
 Cellular Localization    Nucleus
 Entrez Gene Code    7080
 Pathway    Cancer Markers
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 Preparation and Storage    Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.
 Other Notes    Small volumes of anti-TTF-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-TTF-1 antibody

   Thyroid transcription factor-1 (TTF-1), also known as NK2 homeobox 1(NKX2-1), is a member of the NKx2 family of homeodomain transcription factors. It binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. TTF-1 is expressed in epithelial cells of the thyroid gland and the lung, and their derived tumors such as lung adenocarcinoma and small cell carcinomas and thyroid cancer. It is absent in mesotheliomas, breast cancer, and colon cancer.
 Applications Tested/Suitable for anti-TTF-1 antibody   

Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS)

 Application Notes for anti-TTF-1 antibody    Immunohistochemistry Procedure
Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 1mM EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system.
Immunohistochemistry Dilution: 1:100
IHC Positive Control: Thyroid, Lung

Flow Cytometry
Recommended Starting Protocol: Dilute the antibody 1:100. Incubate for 30 minutes at 4 degree C. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user.
Flow Cytometry Positive Control: HeLa Cell Line
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 Immunohistochemistry (IHC) of anti-TTF-1 antibody    Human thyroid stained with anti-TTF-1 antibody
anti-TTF-1 antibody Immunohistochemistry (IHC) (IHC) image
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 Flow Cytometry (FC/FACS)|Flow cytometric analysis of rabbit anti-TTF-1 (SP141) antibody in HeLa (green) compare to negative control of rabbit IgG (blue) of anti-TTF-1 antibody    anti-TTF-1 antibody Flow Cytometry (FC/FACS)|Flow cytometric analysis of rabbit anti-TTF-1 (SP141) antibody in HeLa (green) compare to negative control of rabbit IgG (blue) (FC/FACS) image
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NCBI/Uniprot data below describe general gene information for TTF-1. It may not necessarily be applicable to this product.
 NCBI GI #    14245740
 NCBI Accession #    BAB56137.1 [Other Products]
 Molecular Weight    39 kDa (calculated)
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 NCBI Official Full Name    thyroid transcription factor-1, partial
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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