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anti-TLR4 antibody :: Mouse anti-Human Toll Like Receptor 4 (TLR4) Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS2090574
Unit / Price
0.01 mg  /  $100 +1 FREE 8GB USB
0.02 mg  /  $110 +1 FREE 8GB USB
0.05 mg  /  $140 +1 FREE 8GB USB
0.1 mg  /  $170 +1 FREE 8GB USB
0.2 mg  /  $220 +1 FREE 8GB USB
1 mg  /  $495 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Toll Like Receptor 4 (TLR4), Monoclonal Antibody

 Also Known As   

Monoclonal Antibody to Toll Like Receptor 4 (TLR4)

 Product Synonym Names    CD284; TOLL; HToll
 Product Gene Name   

anti-TLR4 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    U93091 mRNA
 3D Structure    ModBase 3D Structure for O00206
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 Clonality    Monoclonal
 Host    Mouse
 Species Reactivity    Human
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 Specificity    The antibody is a mouse monoclonal antibody raised against TLR4. It has been selected for its ability to recognize TLR4 in immunohistochemical staining and western blotting.
 Purity/Purification    Antigen-specific affinity chromatography followed by Protein A affinity chromatography
 Form/Format    Liquid
 Concentration    1mg/mL (lot specific)
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 Buffer Formulation    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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 Preparation and Storage    Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
 Other Notes    Small volumes of anti-TLR4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-TLR4 antibody   

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

 Application Notes for anti-TLR4 antibody    Western blotting: 0.2-2ug/mL;1:500-5000
Immunohistochemistry: 5-20ug/mL;1:50-200
Immunocytochemistry: 5-20ug/mL;1:50-200
Optimal working dilutions must be determined by end user.
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 Western Blot (WB) of anti-TLR4 antibody    (Western Blot; Sample: Rat Testis lysate; Primary Ab: 3ug/ml Mouse Anti-Human TLR4 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )
anti-TLR4 antibody Western Blot (WB) (WB) image
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 Western Blot (WB) of anti-TLR4 antibody    (Figure. Western Blot; Sample: Recombinant protein.)
anti-TLR4 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for TLR4. It may not necessarily be applicable to this product.
 NCBI GI #    4507533
 NCBI GeneID    7099
 NCBI Accession #    NP_003257.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_003266.3 [Other Products]
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 UniProt Primary Accession #    O00206 [Other Products]
 UniProt Secondary Accession #    Q5VZI8; Q5VZI9; Q9UK78; Q9UM57; A8K1Y8; A9XLP9; A9XLQ0; A9XLQ1; B4E194; D1CS52; D1CS53 [Other Products]
 UniProt Related Accession #    O00206 [Other Products]
 Molecular Weight    73,301 Da
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 NCBI Official Full Name    toll-like receptor 4 isoform C
 NCBI Official Synonym Full Names    toll like receptor 4
 NCBI Official Symbol    TLR4 [Similar Products]
 NCBI Official Synonym Symbols   
TOLL; CD284; TLR-4; ARMD10
[Similar Products]
 NCBI Protein Information    toll-like receptor 4
 UniProt Protein Name    Toll-like receptor 4
 UniProt Synonym Protein Names   
hToll; CD_antigen: CD284
 Protein Family    Toll-like receptor
 UniProt Gene Name    TLR4 [Similar Products]
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 NCBI Summary for TLR4    The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
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 UniProt Comments for TLR4    TLR4: Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS- independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific. Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR4. Binding to bacterial LPS leads to homodimerization. Interacts with LY96 via the extracellular domain. Interacts with MYD88 and TIRAP via their respective TIR domains. Interacts with NOX4. Interacts with CNPY3. Interacts with HSP90B1. The interaction with both CNPY3 and HSP90B1 is required for proper folding in the endoplasmic reticulum. Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells. Belongs to the Toll-like receptor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 9q33.1

Cellular Component: cell surface; cytoplasm; endosome membrane; external side of plasma membrane; integral component of plasma membrane; intracellular; intrinsic component of plasma membrane; lipopolysaccharide receptor complex; perinuclear region of cytoplasm; plasma membrane; receptor complex

Molecular Function: lipopolysaccharide binding; lipopolysaccharide receptor activity; protein binding; protein heterodimerization activity; receptor activity; receptor binding; transmembrane receptor activity

Biological Process: activation of MAPK activity; astrocyte development; B cell proliferation involved in immune response; defense response to bacterium; defense response to Gram-negative bacterium; detection of fungus; detection of lipopolysaccharide; I-kappaB kinase/NF-kappaB signaling; I-kappaB phosphorylation; immune response; inflammatory response; innate immune response; interferon-gamma production; interleukin-1 beta secretion; lipopolysaccharide-mediated signaling pathway; macrophage activation; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of interferon-gamma production; negative regulation of interleukin-17 production; negative regulation of interleukin-23 production; negative regulation of interleukin-6 production; negative regulation of MyD88-independent toll-like receptor signaling pathway; negative regulation of osteoclast differentiation; negative regulation of tumor necrosis factor production; nitric oxide production involved in inflammatory response; positive regulation of B cell proliferation; positive regulation of chemokine production; positive regulation of inflammatory response; positive regulation of interferon-alpha production; positive regulation of interferon-beta biosynthetic process; positive regulation of interferon-beta production; positive regulation of interferon-gamma production; positive regulation of interleukin-1 beta secretion; positive regulation of interleukin-1 production; positive regulation of interleukin-10 production; positive regulation of interleukin-12 biosynthetic process; positive regulation of interleukin-12 production; positive regulation of interleukin-6 production; positive regulation of interleukin-8 biosynthetic process; positive regulation of interleukin-8 production; positive regulation of JNK cascade; positive regulation of macrophage activation; positive regulation of MHC class II biosynthetic process; positive regulation of NF-kappaB import into nucleus; positive regulation of NF-kappaB transcription factor activity; positive regulation of nitric oxide biosynthetic process; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tumor necrosis factor biosynthetic process; positive regulation of tumor necrosis factor production; regulation of dendritic cell cytokine production; response to lipopolysaccharide; T-helper 1 type immune response; toll-like receptor 4 signaling pathway; toll-like receptor signaling pathway

Disease: Macular Degeneration, Age-related, 10
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 Research Articles on TLR4    1. TLR3 or TLR4 activation of mesenchymal stem cells increases Treg cell induction via the Notch pathway
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-TLR4 antibodyDiseases associated with anti-TLR4 antibody
 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway antibodies  Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 HIF-1 Signaling Pathway antibodies  HIF-1 Signaling Pathway Diagram
 Hepatitis B Pathway antibodies  Hepatitis B Pathway Diagram
 IKK Complex Recruitment Mediated By RIP1 Pathway antibodies  IKK Complex Recruitment Mediated By RIP1 Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >3097 publications with TLR4 and Inflammation
 Necrosis Antibodies  >2154 publications with TLR4 and Necrosis
 Sepsis Antibodies  >678 publications with TLR4 and Sepsis
 Neoplasms Antibodies  >496 publications with TLR4 and Neoplasms
 Liver Diseases Antibodies  >357 publications with TLR4 and Liver Diseases
 Atherosclerosis Antibodies  >343 publications with TLR4 and Atherosclerosis
 Drug Toxicity Antibodies  >332 publications with TLR4 and Drug Toxicity
 Heart Diseases Antibodies  >219 publications with TLR4 and Heart Diseases
 Fibrosis Antibodies  >175 publications with TLR4 and Fibrosis
 Carcinoma Antibodies  >170 publications with TLR4 and Carcinoma
Organs/Tissues associated with anti-TLR4 antibody
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >3292 publications with TLR4 and Blood
 Lung Antibodies  >805 publications with TLR4 and Lung
 Liver Antibodies  >729 publications with TLR4 and Liver
 Bone Antibodies  >723 publications with TLR4 and Bone
 Bone Marrow Antibodies  >616 publications with TLR4 and Bone Marrow
 Vascular Antibodies  >504 publications with TLR4 and Vascular
 Intestine Antibodies  >457 publications with TLR4 and Intestine
 Brain Antibodies  >447 publications with TLR4 and Brain
 Spleen Antibodies  >365 publications with TLR4 and Spleen
 Kidney Antibodies  >353 publications with TLR4 and Kidney
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