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Rabbit anti-Human Topoisomerase IIa Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS370150
Unit / Price
5 Control Slides  /  $130 +1 FREE 8GB USB
3 ml (Prediluted)  /  $275 +1 FREE 8GB USB
0.1 mL (Concentrate)  /  $305 +1 FREE 8GB USB
7 ml (Prediluted)  /  $460 +1 FREE 8GB USB
0.5 mL (Concentrate)  /  $665 +1 FREE 8GB USB
15 ml (Prediluted)  /  $750 +1 FREE 8GB USB
1 mL (Concentrate)  /  $930 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Topoisomerase IIa, Monoclonal Antibody

★Popular Item★
 Also Known As   

Topoisomerase IIa RMab

 Product Synonym Names    Topoisomerase IIa RMab
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Monoclonal
 Isotype    IgG
 Clone Number    RBT-Topo2a
 Host    Rabbit
 Species Reactivity    Human
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 Form/Format    Topo IIa is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
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 Control    Cervical, Breast Cancer
 Localization    Nuclear
 Reactivity Note    Paraffin, Frozen
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 Preparation and Storage    Store at 2 to 8 degree C in the dark.
 Other Notes    Small volumes of anti-Topoisomerase IIa antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-Topoisomerase IIa antibody

   DNA Topoisomerase II alpha (Topo IIa) is a nucleic enzyme that affects the topological structure of DNA by interacting with the double-helix DNA, thus playing an important role in DNA replication, transcription, recombination, condensation, and segregation. Type II topoisomerases cut both strands of the DNA helix simultaneously in order to change the linking number of the molecule. Topo IIa is essential in the separation of daughter strands at the end of replication. Failure to separate these strands leads to cell death. In cancers, the Topo IIa is highly expressed in highly-proliferating cells.Topo IIa has been identified by DNA microarray and transcriptional profiling as a gene that is overexpressed in Cervical Carcinomas. The TOP2A gene is approximately 30 kb in size and encodes a 170 kDa protein.Topo IIa protein is expressed in proliferating cells and in numerous human malignant tumors, including colon, gastric and breast cancers, Lymphomas and others. In certain cancers, such as Peripheral Nerve Sheath Tumors, high expression of this protein is also associated with poor patient survival. Type II topoisomerases are the targets for anticancer drugs, such as topoisomerase II inhibitor therapies like the anthracyclines (Doxorubicin and Epirubicin).
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 Dilution Information of anti-Topoisomerase IIa antibody    anti-Topoisomerase IIa antibody Dilution Information image
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 Immunohistochemistry (IHC) of anti-Topoisomerase IIa antibody    anti-Topoisomerase IIa antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemical Protocol of anti-Topoisomerase IIa antibody    Recommended Immunohistochemical Protocol
Pretreatment
1. Cut and mount 3-4 micron formalin-fixed paraffin-embedded tissues on positive charged slides.
2. Air dry for 2 hours at 58 C.
3. Oeparaffinize, dehydrate and rehydrate tissues.
4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate or EDTA.
5. Any of three heating methods may be used:
a. Electric Pressure Cooker
Place standoff rack at base of pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high, and incubate for 15 minutes. Open and immediately transfer slides toroom temperature.
b. Water Bath Method
Place tissues/slides in a pre-warmed staining dish or coplin jar containing the Immuno DNA Retriever with Citrate or EDTA in a water bath set at 95-99 C.lncubate for 30-60 minutes.
c. Conventional Steamer Method
Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes.
6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.
7. Wash slides with IHC wash buffer or 01 water.
8. Continue IHC staining protocol. Immunohistochemical Protocol


anti-Topoisomerase IIa antibody Immunohistochemical Protocol image
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NCBI/Uniprot data below describe general gene information for Topoisomerase IIa. It may not necessarily be applicable to this product.
 NCBI GI #    3869316
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 NCBI Official Full Name    topoisomerase II alpha
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 NCBI Summary for Topoisomerase IIa    This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul 2010]
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-Topoisomerase IIa antibodyPathways associated with anti-Topoisomerase IIa antibody
 Reference Product  PubMed Publications
 FLNA antibody  >12 publications with Topoisomerase IIa and FLNA
 Products by Pathway  Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
Organs/Tissues associated with anti-Topoisomerase IIa antibody
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with Topoisomerase IIa and Bone
 Connective Tissue Antibodies  >4 publications with Topoisomerase IIa and Connective Tissue
 Heart Antibodies  >3 publications with Topoisomerase IIa and Heart
 Muscle Antibodies  >3 publications with Topoisomerase IIa and Muscle
 Brain Antibodies  >3 publications with Topoisomerase IIa and Brain
 Vascular Antibodies  >2 publications with Topoisomerase IIa and Vascular
 Embryonic Tissue Antibodies  >2 publications with Topoisomerase IIa and Embryonic Tissue
 Ear Antibodies  >1 publications with Topoisomerase IIa and Ear
 Kidney Antibodies  >1 publications with Topoisomerase IIa and Kidney
 Umbilical Cord Antibodies  >1 publications with Topoisomerase IIa and Umbilical Cord
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