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anti-VIL1 antibody :: Rabbit Villin Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS302698
Unit / Price
0.1 mL (Concentrate)  /  $270 +1 FREE 8GB USB
7 mL (RTU)  /  $440 +1 FREE 8GB USB
0.5 mL (Concentrate)  /  $495 +1 FREE 8GB USB
1 mL (Concentrate)  /  $730 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Villin (VIL1), Monoclonal Antibody

 Also Known As   

Rabbit Anti-Human Villin Monoclonal Antibody (Clone SP145)

 Product Gene Name   

anti-VIL1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    193040
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 Clonality    Monoclonal
 Isotype    IgG
 Clone Number    SP145
 Host    Rabbit
 Species Reactivity    Human
Predicted Reactivity: Bovine, Chicken, Mouse, Pig
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 Form/Format    0.1 ml rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.
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 Immunogen    Synthetic peptide derived from the C-terminus of human villin protein.
 Control Tissue    Colon, Kidney, Small Intestine
 Cellular Localization    Cytoplasm
 Entrez Gene Code    7429
 Pathway    Cytoskeletal Proteins
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 Preparation and Storage    Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.
 Other Notes    Small volumes of anti-VIL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-VIL1 antibody

   Villin is part of cytoskeleton associated with the microvillar actin core bundle of gastrointestinal and renal brush border. It can interact with actin in a Ca2+ and phosphoinositide-regulated manner. Villin is a very specific marker for adenocarcinomas of the gastrointestinal tract and the pancreas. It is also expressed in some Merkel cell tumor and adenocarcinoma of the lung, prostate, ovarian and kidney.
 Applications Tested/Suitable for anti-VIL1 antibody   

Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

 Application Notes for anti-VIL1 antibody    Immunohistochemistry Procedure
Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system.
Immunohistochemistry Dilution: 1:100
IHC Positive Control: Colon, Small Intestine, Kidney

Western Blot
Recommended Starting Protocol: Dilute the antibody 1:100. Incubate for 1 hour at room temperature. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user.
Western Blot Positive Control: HT-29 Cell Lysate
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 Western Blot (WB) of anti-VIL1 antibody    Western blot analysis of HT-29 cell lysate with villin antibody
anti-VIL1 antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-VIL1 antibody    Human colon stained with anti-villin antibody
anti-VIL1 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for VIL1. It may not necessarily be applicable to this product.
 NCBI GI #    194394237
 NCBI GeneID    7429
 NCBI Accession #    NP_009058.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_007127.2 [Other Products]
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 UniProt Secondary Accession #    Q53S11; Q96AC8; B2R9A7 [Other Products]
 UniProt Related Accession #    P09327 [Other Products]
 Molecular Weight    95 kDa [Similar Products]
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 NCBI Official Full Name    villin-1
 NCBI Official Synonym Full Names    villin 1
 NCBI Official Symbol    VIL1 [Similar Products]
 NCBI Official Synonym Symbols   
VIL; D2S1471
[Similar Products]
 NCBI Protein Information    villin-1
 UniProt Protein Name    Villin-1
 Protein Family    Villin
 UniProt Gene Name    VIL1 [Similar Products]
 UniProt Synonym Gene Names    VIL [Similar Products]
 UniProt Entry Name    VILI_HUMAN
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 NCBI Summary for VIL1    This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq, Jul 2008]
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 UniProt Comments for VIL1    Villin: Epithelial cell-specific Ca(2+)-regulated actin- modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination. Monomer. Homodimer; homodimerization is necessary for actin-bundling. Associates with F-actin; phosphorylation at tyrosines residues decreases the association with F-actin. Interacts (phosphorylated at C-terminus tyrosine phosphorylation sites) with PLCG1 (via the SH2 domains). Interacts (phosphorylated form) with PLCG1; the interaction is enhanced by hepatocyte growth factor (HGF). Specifically expressed in epithelial cells. Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells. Expressed in canalicular microvilli of hepatocytes. Belongs to the villin/gelsolin family.

Protein type: Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: ruffle; filopodium tip; microvillus; lamellipodium; cytoplasm; actin filament bundle; filopodium

Molecular Function: actin filament binding; identical protein binding; phosphatidylinositol-4,5-bisphosphate binding; protein binding; protein homodimerization activity; caspase inhibitor activity; calcium ion binding

Biological Process: epidermal growth factor receptor signaling pathway; actin filament depolymerization; actin filament severing; actin filament capping; negative regulation of caspase activity; actin nucleation; regulation of cell shape; response to bacterium; epithelial cell differentiation; actin filament polymerization; positive regulation of actin filament bundle formation; regulation of actin nucleation; protein complex assembly; positive regulation of cell migration
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 Research Articles on VIL1    1. Discriminated small-cell lung carcinoma from large-cell neuroendocrine lung carcinoma with immunohistochemical markers BAI3, CDX2 and VIL1.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-VIL1 antibodyPathways associated with anti-VIL1 antibody
 Reference Product  PubMed Publications
 TNP1 antibody  >4 publications with VIL1 and TNP1
 Products by Pathway  Pathway Diagram
 Regulation Of Actin Cytoskeleton Pathway antibodies  Regulation Of Actin Cytoskeleton Pathway Diagram
Diseases associated with anti-VIL1 antibodyOrgans/Tissues associated with anti-VIL1 antibody
 Disease Name  Pubmed Publications
 Uterine Cervical Neoplasms Antibodies  >3 publications with VIL1 and Uterine Cervical Neoplasms
 Liver Diseases Antibodies  >3 publications with VIL1 and Liver Diseases
 Liver Neoplasms Antibodies  >2 publications with VIL1 and Liver Neoplasms
 Lung Neoplasms Antibodies  >1 publications with VIL1 and Lung Neoplasms
 Carcinoma, Hepatocellular Antibodies  >1 publications with VIL1 and Carcinoma, Hepatocellular
 Inflammation Antibodies  >1 publications with VIL1 and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >3 publications with VIL1 and Muscle
 Liver Antibodies  >3 publications with VIL1 and Liver
 Intestine Antibodies  >2 publications with VIL1 and Intestine
 Embryonic Tissue Antibodies  >1 publications with VIL1 and Embryonic Tissue
 Uterus Antibodies  >1 publications with VIL1 and Uterus
 Brain Antibodies  >1 publications with VIL1 and Brain
 Lung Antibodies  >1 publications with VIL1 and Lung
 Kidney Antibodies  >1 publications with VIL1 and Kidney
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