View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List Mouse anti-Human, Mouse WAF1, p21 Monoclonal Antibody Catalog # MBS601175 Unit / Price 0.2 mg / $870 +1 FREE 8GB USB 5x0.2 mg / $3,750 +4 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name WAF1, p21, Monoclonal Antibody Full Product Name WAF1, p21 (Wild-type p53-activated Fragment, Cip1, Sdi1, Pic1 Tumor Suppressor Protein) Product Synonym Names Anti -WAF1, p21 (Wild-type p53-activated Fragment, Cip1, Sdi1, Pic1 Tumor Suppressor Protein) Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Monoclonal Isotype IgG1,k+2b,k Clone Number 0.T.180/0.T.180A Host Mouse Species Reactivity Human, Mouse Specificity Recognizes WAF1, p21,~ 21kD. Species crossreactivity: mouse. Purity/Purification Affinity PurifiedPurified by Protein G affinity chromatography. Form/Format Supplied as liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide. Immunogen Recombinant full-length WAF1, p21 (Wild-type p53-activated Fragment, Cip1) with a histidine tag. Positive Control HeLa nuclear extract. Use 20ug per lane for minigels. Preparation and Storage -20 degree C Other Notes Small volumes of anti-WAF1, p21 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for anti-WAF1, p21 antibody Expression of p21WAF1 is induced by wild type, but not mutant, p53 suppressor protein. The p21WAF1 protein binds to cyclin/CDK complexes and inhibits their kinase activity thereby stopping cell cycle progression. Meanwhile, the p21WAF1 protein also binds to PCNA (proliferating cell nuclear antigen) and blocks DNA replication but not the DNA repair process. Evidently, inactivation of p53 disrupts the p53-dependent expression of p21WAF1. p21WAF1is a tumor suppressor involved in the pathogenesis of a variety of malignancies.Pooled monoclonal mouse IgGs Product Categories/Family for anti-WAF1, p21 antibody Antibodies; Abs to Cancer Markers Applications Tested/Suitable for anti-WAF1, p21 antibody Western Blot (WB), Immunoprecipitation (IP) Application Notes for anti-WAF1, p21 antibody Suitable for use in Immunoprecipitation, Western Blot.Immunoprecipitation: 4ug immunoprecipitates Cip1 from 500ug of HeLa nuclear extract. Western Blot (WB) of anti-WAF1, p21 antibody WB: HeLa cell nuclear extract probed with MBS601175 (1ug/ml) TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP