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anti-WT1 antibody :: Mouse Wilm's Tumor 1 (WT1) Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS439618
Unit / Price
0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $190 +1 FREE 8GB USB
7 ml (Prediluted)  /  $270 +1 FREE 8GB USB
0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $300 +1 FREE 8GB USB
0.1 mg (Without BSA & Azide at 1mg/ml)  /  $310 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Wilm's Tumor 1 (WT1), Monoclonal Antibody

★Popular Item★
 Also Known As   

Wilm's Tumor 1 (WT1) (Wilm's Tumor & Mesothelial Marker) Mouse Monoclonal Antibody

 Product Synonym Names    WT1; AWT1; FWT1; GUD; NPHS4; WAGR; Wilms tumor 1
 Product Gene Name   

anti-WT1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 MBS439618 Technical Datasheet    Technical Datasheet PDF
 OMIM    136680
 3D Structure    ModBase 3D Structure for P19544
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 Clonality    Monoclonal
 Isotype    IgG1, kappa
 Clone Number    WT1/857
 Host    Mouse
 Species Reactivity    Human and Rat. Weakly reacts with Mouse. Others-not known
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 Specificity    Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm’s tumors. WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm’s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm’s tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.
 Form/Format    200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
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 Immunogen    Recombinant full-length human WT1 protein
 Cellular Localization    Nuclear and Cytoplasmic
    
 Human Chromosome Location    11p13
 Positive Control    K562 cells. Kidney, Testis, Wilm’s Tumor or Mesothelioma.
 Supplied As    200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
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 Preparation and Storage    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
 Other Notes    Small volumes of anti-WT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-WT1 antibody   

Immunohistology (Formalin-fixed)

 Application Notes for anti-WT1 antibody    Suggested Dilutions: Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
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 Immunohistology (IHC) of anti-WT1 antibody    Formalin-fixed, paraffin-embedded Human Mesothelioma stained with Wilm’s Tumor Mouse Monoclonal Antibody (WT1/857).
anti-WT1 antibody Immunohistology (IHC) image
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 SDS-PAGE of anti-WT1 antibody    SDS-PAGE Analysis Purified Wilm’s Tumor Mouse Monoclonal Antibody (WT1/857). Confirmation of Integrity and Purity of Antibody.
anti-WT1 antibody SDS-PAGE image
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 Immunohistology (IHC) of anti-WT1 antibody    Formalin-fixed, paraffin-embedded Human Testis stained with Wilm’s Tumor Mouse Monoclonal Antibody (WT1/857).
anti-WT1 antibody Immunohistology (IHC) image
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 Immunohistology (IHC) of anti-WT1 antibody    Formalin-fixed, paraffin-embedded Rat Testis stained with Wilm’s Tumor Mouse Monoclonal Antibody (WT1/857).
anti-WT1 antibody Immunohistology (IHC) image
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NCBI/Uniprot data below describe general gene information for WT1. It may not necessarily be applicable to this product.
 NCBI GI #    65507714
 NCBI GeneID    7490
 NCBI Accession #    NP_000369.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_000378.4 [Other Products]
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 UniProt Primary Accession #    P19544 [Other Products]
 UniProt Secondary Accession #    Q15881; Q16256; Q16575; Q4VXV4; Q4VXV5; Q4VXV6; Q8IYZ5; A8K6S1; B3KSA5 [Other Products]
 UniProt Related Accession #    P19544 [Other Products]
 Molecular Weight    47-55kDa
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 NCBI Official Full Name    Wilms tumor protein isoform A
 NCBI Official Synonym Full Names    Wilms tumor 1
 NCBI Official Symbol    WT1 [Similar Products]
 NCBI Official Synonym Symbols   
GUD; AWT1; WAGR; WT33; NPHS4; WIT-2; EWS-WT1
[Similar Products]
 NCBI Protein Information    Wilms tumor protein
 UniProt Protein Name    Wilms tumor protein
 UniProt Synonym Protein Names   
WT33
 Protein Family    Wilms tumor protein
 UniProt Gene Name    WT1 [Similar Products]
 UniProt Entry Name    WT1_HUMAN
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 NCBI Summary for WT1    This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015]
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 UniProt Comments for WT1    Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:7862533, PubMed:17716689, PubMed:25258363). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921).
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Product References and Citations for anti-WT1 antibody

   Rauscher FJ. The WT1 Wilms tumor gene product: A developmentally regulated transcription factor in the kidney that functions as a tumor suppressor. FASEB J 1993; 7:896
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 Research Articles on WT1    1. We present WT1 as a robust prognostic marker in high-grade serous ovarian carcinoma, which adds prognostic information to ER-alpha and should be kept in mind when WT1 is used as a biomarker in the context of WT1-targeting therapies.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-WT1 antibodyPathways associated with anti-WT1 antibody
 Reference Product  PubMed Publications
 PAX2 antibody  >110 publications with WT1 and PAX2
 SOX9 antibody  >108 publications with WT1 and SOX9
 TP53 antibody  >67 publications with WT1 and TP53
 NPHS1 antibody  >53 publications with WT1 and NPHS1
 PAX8 antibody  >52 publications with WT1 and PAX8
 WNT4 antibody  >50 publications with WT1 and WNT4
 WTAP antibody  >34 publications with WT1 and WTAP
 NR0B1 antibody  >34 publications with WT1 and NR0B1
 PAWR antibody  >6 publications with WT1 and PAWR
 FOXD1 antibody  >2 publications with WT1 and FOXD1
 Products by Pathway  Pathway Diagram
 Integrated Pancreatic Cancer Pathway antibodies  Integrated Pancreatic Cancer Pathway Diagram
 Regulation Of Telomerase Pathway antibodies  Regulation Of Telomerase Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
Diseases associated with anti-WT1 antibodyOrgans/Tissues associated with anti-WT1 antibody
 Disease Name  Pubmed Publications
 Wilms Tumor Antibodies  >2022 publications with WT1 and Wilms Tumor
 Kidney Diseases Antibodies  >970 publications with WT1 and Kidney Diseases
 Leukemia Antibodies  >848 publications with WT1 and Leukemia
 Leukemia, Myeloid, Acute Antibodies  >487 publications with WT1 and Leukemia, Myeloid, Acute
 Denys-Drash Syndrome Antibodies  >204 publications with WT1 and Denys-Drash Syndrome
 Breast Neoplasms Antibodies  >127 publications with WT1 and Breast Neoplasms
 Lung Neoplasms Antibodies  >118 publications with WT1 and Lung Neoplasms
 Renal Insufficiency Antibodies  >106 publications with WT1 and Renal Insufficiency
 Glomerulonephritis Antibodies  >99 publications with WT1 and Glomerulonephritis
 Disease Models, Animal Antibodies  >98 publications with WT1 and Disease Models, Animal
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >1051 publications with WT1 and Kidney
 Blood Antibodies  >696 publications with WT1 and Blood
 Bone Antibodies  >338 publications with WT1 and Bone
 Testis Antibodies  >182 publications with WT1 and Testis
 Lung Antibodies  >171 publications with WT1 and Lung
 Ovary Antibodies  >151 publications with WT1 and Ovary
 Brain Antibodies  >102 publications with WT1 and Brain
 Prostate Antibodies  >65 publications with WT1 and Prostate
 Uterus Antibodies  >55 publications with WT1 and Uterus
 Intestine Antibodies  >19 publications with WT1 and Intestine
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