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anti-Zap70 antibody :: Mouse anti-Human ZAP-70 (TCR zeta) Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS670112 anti-Zap70 antibody
Unit / Price
25 mg  /  $165 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ZAP-70 (TCR zeta) (Zap70), Monoclonal Antibody

 Also Known As   

Mouse Anti-Human ZAP-70-BIOT

 Product Synonym Names    Mouse Anti-Human ZAP-70 - Biotin; Mouse Anti-Human ZAP-70 (TCR zeta)
 Product Gene Name   

anti-Zap70 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 1; NC_000067.5 (36818706..36839661). Location: 1 A4-C1; 1 20.5 cM
 3D Structure    ModBase 3D Structure for P43404
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 Clonality    Monoclonal
 Isotype    Mouse IgG1 kappa
 Clone Number    SBZAP
 Host    Mouse
 Species Reactivity    Human
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 Specificity    Human TCR zeta ; Zap-70 (Mr 70 kDa)
ZAP 70, also known as TCR zeta associated protein, is a protein tyrosine kinase (PTK) with the primary role of catalyzing the phosphorylation of tyrosine residues in cellular proteins, and thus, contributes significantly to the initiation of cell signaling. ZAP 70 is expressed on T cells and NK cells. The monoclonal antibodies SB70 and SBZAP were generated against a KLH-peptide sequence corresponding to the human ZAP 70 amino acid residues 280-309.
 Form/Format    Biotin (BIOT) Conjugate
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 Antigen Description    Human ZAP-70 amino acid residues 280-309
 Characterization    To insure lot-to-lot consistency, each batch of product is tested by ELISA and/or PCFIA assay to conform to the characteristics of a standard reference reagent.
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 Preparation and Storage    The purified antibody is supplied as 0.025 mg purified immunoglobulin in 0.25 mL or 0.1 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or aminecontaining buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.025 mg in 0.25 mL or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at - 20 degree C.
The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
The biotin (BIOT) conjugate is supplied as 0.025 mg in 0.25 mL or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.025 mg in 0.25 mL or 0.1 mg in 1.0 mL in PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
 Other Notes    Small volumes of anti-Zap70 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-Zap70 antibody   

ELISA; Flow Cytometry

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NCBI/Uniprot data below describe general gene information for Zap70. It may not necessarily be applicable to this product.
 NCBI GI #    436480
 NCBI GeneID    22637
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 UniProt Primary Accession #    P43404 [Other Products]
 UniProt Secondary Accession #    P97455; Q80VV2; Q8CHJ3 [Other Products]
 UniProt Related Accession #    P43404 [Other Products]
 Molecular Weight    70,058 Da [Similar Products]
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 NCBI Official Full Name    ZAP-70
 NCBI Official Synonym Full Names    zeta-chain (TCR) associated protein kinase
 NCBI Official Symbol    Zap70 [Similar Products]
 NCBI Official Synonym Symbols   
Srk; TZK; mur; 70kDa; mrtle; ZAP-70; AI327364
[Similar Products]
 NCBI Protein Information    tyrosine-protein kinase ZAP-70; syk-related tyrosine kinase; 70 kDa zeta-associated protein
 UniProt Protein Name    Tyrosine-protein kinase ZAP-70
 UniProt Synonym Protein Names   
70 kDa zeta-associated protein; Syk-related tyrosine kinase
 Protein Family    Tyrosine-protein kinase
 UniProt Gene Name    Zap70 [Similar Products]
 UniProt Synonym Gene Names    Srk; Zap-70 [Similar Products]
 UniProt Entry Name    ZAP70_MOUSE
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 NCBI Summary for Zap70    This gene encodes a member of the protein tyrosine kinase family. The encoded protein is essential for development of T lymphocytes and thymocytes, and functions in the initial step of T lymphocyte receptor-mediated signal transduction. A mutation in this gene causes chronic autoimmune arthritis, similar to rheumatoid arthritis in humans. Mice lacking this gene are deficient in alpha-beta T lymphocytes in the thymus. In humans, mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T lymphocytes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
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 UniProt Comments for Zap70    Function: Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not. Ref.3

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subunit structure: Interacts with CBL. Interacts with SHB. Interacts with FCRL3

By similarity. Interacts with CD3Z and with phosphorylated NFAM1. Interacts with SLA and SLA2 when it is phosphorylated. The association with SLA (or SLA2) and CBL probably leads to its destruction. Interacts with CBLB and DEF6. Interacts (via SH2 domains) with RHOH. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10

Subcellular location: Cytoplasm. Cell membrane. Note: After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes. Ref.3 Ref.10

Tissue specificity: Isoform 1 and isoform 2 are expressed in thymus, sleen and lymph nodes. Ref.3

Developmental stage: Isoform 2 is expressed in develoing thymocytes from the CD44+CD25- stage up to mature T cells. Isoform 1 is not expressed in thymocytes at the CD44+CD25- or CD44+CD25+ stages. Ref.3

Domain: The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH. Ref.10

Post-translational modification: Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-318 phosphorylation is essential for full activity

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.Contains 1 protein kinase domain.Contains 2 SH2 domains.
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Product References and Citations for anti-Zap70 antibody

   1. Wange, R.L., N. Isakov, T.R. Burke., A. Otaka, P.P. Roller, J.D. Watts, R. Aebersold, and L.E. Samelson. 1995. J. Biol. Chem. 270:944.
3. Van Oers, N.S., and A. Weiss. 1995. Semin. Immunol. 7:227.
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 Research Articles on Zap70    1. Sts-2 is a phosphatase that negatively regulates zeta-associated protein (ZAP)-70 and T cell receptor signaling pathways.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-Zap70 antibodyDiseases associated with anti-Zap70 antibody
 Products by Pathway  Pathway Diagram
 Adaptive Immunity Signaling Pathway antibodies  Adaptive Immunity Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 Generation Of Second Messenger Molecules Pathway antibodies  Generation Of Second Messenger Molecules Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Inflammatory Response Pathway antibodies  Inflammatory Response Pathway Diagram
 Natural Killer Cell Mediated Cytotoxicity Pathway antibodies  Natural Killer Cell Mediated Cytotoxicity Pathway Diagram
 Natural Killer Cell Mediated Cytotoxicity Pathway antibodies  Natural Killer Cell Mediated Cytotoxicity Pathway Diagram
 Primary Immunodeficiency Pathway antibodies  Primary Immunodeficiency Pathway Diagram
 Primary Immunodeficiency Pathway antibodies  Primary Immunodeficiency Pathway Diagram
 T Cell Receptor Signaling Pathway antibodies  T Cell Receptor Signaling Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >331 publications with Zap70 and Neoplasms
 Lymphatic Diseases Antibodies  >291 publications with Zap70 and Lymphatic Diseases
 Lymphoma Antibodies  >62 publications with Zap70 and Lymphoma
 Immunologic Deficiency Syndromes Antibodies  >54 publications with Zap70 and Immunologic Deficiency Syndromes
 Severe Combined Immunodeficiency Antibodies  >35 publications with Zap70 and Severe Combined Immunodeficiency
 Inflammation Antibodies  >28 publications with Zap70 and Inflammation
 Hematologic Diseases Antibodies  >24 publications with Zap70 and Hematologic Diseases
 Necrosis Antibodies  >24 publications with Zap70 and Necrosis
 Vascular Diseases Antibodies  >10 publications with Zap70 and Vascular Diseases
 Cell Transformation, Neoplastic Antibodies  >9 publications with Zap70 and Cell Transformation, Neoplastic
Organs/Tissues associated with anti-Zap70 antibody
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >688 publications with Zap70 and Blood
 Thymus Antibodies  >62 publications with Zap70 and Thymus
 Spleen Antibodies  >34 publications with Zap70 and Spleen
 Brain Antibodies  >3 publications with Zap70 and Brain
 Mammary Gland Antibodies  >2 publications with Zap70 and Mammary Gland
 Salivary Gland Antibodies  >1 publications with Zap70 and Salivary Gland
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