P43404
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UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
ZAP-70
NCBI Official Synonym Full Names
zeta-chain (TCR) associated protein kinase
NCBI Official Synonym Symbols
Srk; TZK; mur; 70kDa; mrtle; ZAP-70; AI327364 [Similar Products]
NCBI Protein Information
tyrosine-protein kinase ZAP-70; syk-related tyrosine kinase; 70 kDa zeta-associated protein
UniProt Protein Name
Tyrosine-protein kinase ZAP-70
UniProt Synonym Protein Names
70 kDa zeta-associated protein; Syk-related tyrosine kinase
UniProt Synonym Gene Names
UniProt Entry Name
ZAP70_MOUSE
NCBI Summary for Zap70
This gene encodes a member of the protein tyrosine kinase family. The encoded protein is essential for development of T lymphocytes and thymocytes, and functions in the initial step of T lymphocyte receptor-mediated signal transduction. A mutation in this gene causes chronic autoimmune arthritis, similar to rheumatoid arthritis in humans. Mice lacking this gene are deficient in alpha-beta T lymphocytes in the thymus. In humans, mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T lymphocytes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
UniProt Comments for Zap70
Function: Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not. Ref.3
Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subunit structure: Interacts with CBL. Interacts with SHB. Interacts with FCRL3
By similarity. Interacts with CD3Z and with phosphorylated NFAM1. Interacts with SLA and SLA2 when it is phosphorylated. The association with SLA (or SLA2) and CBL probably leads to its destruction. Interacts with CBLB and DEF6. Interacts (via SH2 domains) with RHOH. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10
Subcellular location: Cytoplasm. Cell membrane. Note: After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes. Ref.3 Ref.10
Tissue specificity: Isoform 1 and isoform 2 are expressed in thymus, sleen and lymph nodes. Ref.3
Developmental stage: Isoform 2 is expressed in develoing thymocytes from the CD44+CD25- stage up to mature T cells. Isoform 1 is not expressed in thymocytes at the CD44+CD25- or CD44+CD25+ stages. Ref.3
Domain: The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH. Ref.10
Post-translational modification: Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-318 phosphorylation is essential for full activity
Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.Contains 1 protein kinase domain.Contains 2 SH2 domains.
Product References and Citations for anti-Zap70 antibody
1. Wange, R.L., N. Isakov, T.R. Burke., A. Otaka, P.P. Roller, J.D. Watts, R. Aebersold, and L.E. Samelson. 1995. J. Biol. Chem. 270:944. 3. Van Oers, N.S., and A. Weiss. 1995. Semin. Immunol. 7:227.
Research Articles on Zap70
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Pathways associated with anti-Zap70 antibody
Diseases associated with anti-Zap70 antibody
Organs/Tissues associated with anti-Zap70 antibody
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