View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List Mouse anti-Rabbit a-Dystroglycan Monoclonal Antibody Catalog # MBS600655 Unit / Price 0.2 mL / $770 +1 FREE 8GB USB 5x0.2 mL / $3,290 +4 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name a-Dystroglycan, Monoclonal Antibody Popular Item Full Product Name a-Dystroglycan (Cranin) Product Synonym Names Anti -a-Dystroglycan (Cranin) Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Monoclonal Isotype IgG2b Clone Number 4A145 Host Mouse Species Reactivity Rabbit Specificity Recognizes rabbit alpha-Dystroglycan at ~156kD. Species Crossreactivity: human, mouse, rat, canine and guinea pig. Purity/Purification Ascites Form/Format Supplied as a liquid in PBS, 0.05% sodium azide, 30% glycerol. Immunogen Rabbit skeletal muscle membrane preparation. Preparation and Storage May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Other Notes Small volumes of anti-a-Dystroglycan antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for anti-a-Dystroglycan antibody Dystroglycans are essential elements of the neuromuscularjunction (NMJ). The gene for dystroglycan is expressed as aprecursor protein that is post translationally cleaved into a156kD extracellular peripheral membrane protein called alphadystroglycan and a 43kD transmembrane protein, betadystroglycan. The latter protein contains a PPxY motif thatpromotes binding to WW domain containing proteins, such asutrophin and dystrophin. Phosphorylation at tyrosine 892within the PPxY motif may regulate c-Src interactions with betadystroglycan, as well as inhibit interactions with WW domainproteins. In skeletal muscle, beta dystroglycan is normallylocalized to the plasma membrane, however phosphorylationof Tyr892 leads to localization of beta dystroglycan toendosomal compartments along with c-Src. Thus,phosphorylation at Tyr892 may have important roles in alteringthe localization of beta dystroglycan during NMJ formation Product Categories/Family for anti-a-Dystroglycan antibody Antibodies; Abs to Carbohydrates; Glycoproteins Applications Tested/Suitable for anti-a-Dystroglycan antibody Inhibition, Western Blot (WB) Application Notes for anti-a-Dystroglycan antibody Inhibition of Laminin Binding to Dystroglycan:In anitrocellulose overlay experiment, this antibody inhibits bindingof 125I Laminin to Dystroglycan.Western Blot: 1:1000-1:2000 Detects alpha-Dystroglycan inrabbit skeletal muscle. Rabbit skeletal muscle lysate wasresolved by electrophoresis, transferred to nitrocellulose andprobed with D9905-05. Proteins were visualized using an IgG(HRP) goat anti-mouse and a chemiluminescence detectionsystem.Note:The use of WGA purified protein results in significantlycleaner blots and immunoprecipitates. Post-translationalmodification of Dystroglycan causes band broadening. Optimal dilutions to be determined by the researcher. Other applications not tested. Western Blot (WB) of anti-a-Dystroglycan antibody Western Blot analysis of rabbit skeletal muscle lysate using MBS600655 (1:2000). TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP