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Mouse a-Dystroglycan Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS603708 anti-a-Dystroglycan antibody
Unit / Price
0.2 mL  /  $730 +1 FREE 8GB USB
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 Product Name   

a-Dystroglycan, Monoclonal Antibody

★Popular Item★
 Also Known As   

a-Dystroglycan (Cranin)

 Product Synonym Names    Anti -a-Dystroglycan (Cranin)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    0.T.29
 Host    Mouse
 Species Reactivity    Canine, Guinea Pig, Human, Mouse, Rabbit, Rat
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 Specificity    Recognizes rabbit alpha Dystroglycan at ~156kD. Species Crossreactivity: human, rat, guinea pig, canine and mouse.
 Purity/Purification    Supernatant
 Form/Format    Supplied as liquid, 0.05% sodium azide.
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 Immunogen    Rabbit skeletal muscle membrane preparation to alpha Dystroglycan.
 Positive Control    Mouse, rat and human skeletal muscle tissue extracts or DU145 (human prostate carcinoma tumor) cell lysate.
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 Preparation and Storage    May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
 Other Notes    Small volumes of anti-a-Dystroglycan antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-a-Dystroglycan antibody

   Dystroglycan is one of the dystrophin-associated glycoproteins, which is encoded by a 5.5kb transcript in Homo sapiens by chromosome 3. There are two exons that are separated by a large intron. The spliced exons codes for a protein product is finally cleaved into two non-covalently associated subunits, alpha (N-terminal) and beta (C-terminal). In general, aberrant expression of dystrophin-associated protein complex underlies the pathogenesis of Duchenne muscular dystrophy, Becker muscular dystrophy and severe childhood autosomal recessive muscular dystrophy. Interestingly, no genetic disease has been described for either alpha or beta-dystroglycan. Dystroglycan is widely distributed in non-muscle tissues as well as in muscle tissues. During epithelial morphogenesis of kidney, the dystroglycan complex is shown to act as a receptor for the basement membrane. Dystroglycan expression in Mus musculus brain and neural retina has also been reported. However, the physiological role of dystroglycan in non-muscle tissues has remained unclear.
 Product Categories/Family for anti-a-Dystroglycan antibody    Antibodies; Abs to Proteins
 Applications Tested/Suitable for anti-a-Dystroglycan antibody   

ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

 Application Notes for anti-a-Dystroglycan antibody    Suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot.
Dilution: Immunohistochemistry (Frozen): 1:100 detected a-Dystroglycan in frozen rabbit skeletal muscle sections fixed with 50% ethanol and 50% acetic acid.
Immunoprecipitation: 10ul immunprecipitates dystroglycan from 750ug of C2C12 cell lysate.
Western Blot: 1:100-1:1000. Detects a-Dystroglycan in C2C12 cell lysate. Previous lot detected Dystroglycan in rabbit skeletal muscle.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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