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anti-ACTA1 antibody :: Mouse actin alpha-skeletal Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS570258
Unit / Price
0.1 mg  /  $400 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

actin alpha-skeletal (ACTA1), Monoclonal Antibody

 Also Known As   

Mouse anti actin alpha-skeletal

 Product Gene Name   

anti-ACTA1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 1; NC_000001.10 (229566992..229569858, complement). Location: 1q42.13
 OMIM    102610
 3D Structure    ModBase 3D Structure for P68133
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 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    3B3
 Host    Mouse
 Species Reactivity    Caprine, Human, Rabbit, Rat, Swine
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 Specificity    Clone 3B3 is an antibody highly specific for alpha-skeletal actin, and does not cross react with other actin isoforms.
 Form/Format    Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
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 Source Note    3B3 is a Mouse monoclonal IgG1 antibody derived by fusion of NS0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a peptide comprising the N-terminal nonapeptide of alpha-skeletal actin with an acetylated N-terminus (Ac-DEDETTALVC-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue.
 Reactivity Note    The epitope recognized by alpha-SM1 is highly conserved. The antibody therefore cross-reacts with many species including protochordates, lower craniates and mammals.
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 Preparation and Storage    Store at 4 degree C, or in small aliquots at -20 degree C.
 Other Notes    Small volumes of anti-ACTA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-ACTA1 antibody

   Among the six actin isoforms described in mammals, two are found in virtually all cells (beta- and gamma-cytoplasmic), two are detected in smooth muscle cells (alpha- and gamma-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (alpha-skeletal) and one in cardiac (alpha-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus, but the sequence of each of these actins is highly conserved in higher vertebRates. Skeletal alpha-actin may also be expressed in the mammalian heart. Although during the late stage of fetal development alpha-cardiac actin becomes the main isoform in the myocardium, alpha-skeletal actin might still make up for almost half of its total striated muscle actin content.
 Product Categories/Family for anti-ACTA1 antibody    Cytoskeletal, Cardiovascular, Cell differentiation, Cytoskeleton, Stem cell research
 Applications Tested/Suitable for anti-ACTA1 antibody   

ELISA (EIA), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB)

 Application Notes for anti-ACTA1 antibody    3B3 is useful for immunohistochemistry on frozen and paraffin-embedded tissues, immunoblotting and ELISA. Optimal antibody dilutions should be determined by titRation; recommended range is 1:100 - 1:1000 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:1000 - 1:5000 for immunoblotting appliCations.
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 Testing Data of anti-ACTA1 antibody    Paraffin sections of human striated skeletal muscle (above) and striated muscle (below) from human tongue immunostained with 3B3 (1:250)
anti-ACTA1 antibody Testing Data image
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 Testing Data of anti-ACTA1 antibody   
anti-ACTA1 antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for ACTA1. It may not necessarily be applicable to this product.
 NCBI GI #    4501881
 NCBI GeneID    58
 NCBI Accession #    NP_001091.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001100.3 [Other Products]
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 UniProt Primary Accession #    P68133 [Other Products]
 UniProt Secondary Accession #    P02568; P99020; Q5T8M9 [Other Products]
 UniProt Related Accession #    P68133 [Other Products]
 Molecular Weight    42,051 Da [Similar Products]
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 NCBI Official Full Name    actin, alpha skeletal muscle
 NCBI Official Synonym Full Names    actin, alpha 1, skeletal muscle
 NCBI Official Symbol    ACTA1 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    actin, alpha skeletal muscle; nemaline myopathy type 3
 UniProt Protein Name    Actin, alpha skeletal muscle
 UniProt Synonym Protein Names   
 UniProt Gene Name    ACTA1 [Similar Products]
 UniProt Synonym Gene Names    ACTA [Similar Products]
 UniProt Entry Name    ACTS_HUMAN
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 NCBI Summary for ACTA1    The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ACTA1    ACTA1: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID. Interacts (via its C-terminus) with USP25; the interaction occurs for all USP25 isoforms but is strongest for isoform USP25m in muscle differentiating cells. Belongs to the actin family.

Protein type: Cell development/differentiation; Contractile; Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q42.13

Cellular Component: extracellular space; sarcomere; stress fiber; actin filament; cytosol; striated muscle thin filament; actin cytoskeleton

Molecular Function: protein binding; myosin binding; structural constituent of cytoskeleton; ADP binding; ATP binding

Biological Process: skeletal muscle fiber adaptation; muscle contraction; response to steroid hormone stimulus; response to mechanical stimulus; muscle thin filament assembly; response to lithium ion; response to extracellular stimulus; skeletal muscle fiber development; cell growth; muscle filament sliding

Disease: Nemaline Myopathy 3; Myopathy, Congenital, With Fiber-type Disproportion
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Product References and Citations for anti-ACTA1 antibody

   Vandekerckhove, J. and Weber, K. (1978). At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol. 126(4), 783-802.Vandekerckhove, J., Bugaisky, G. and Buckingham, M. (1986). Simultaneous expression of skeletal muscle and heart actin proteins in various striated muscle tissues and cells. J Biol Chem. 261(4),1838-43.Suurmeijer, A.J., Clément, S., Francesconi, A., Bocchi, L., Angelini, A., Van Veldhuisen, D.J., Spagnoli, L.G., Gabbiani, G. and Orlandi, A. (2003). Alpha-actin isoform distribution in normal and failing Human heart: a morphological, morphometric, and biochemical study. J Pathol. 199(3), 387-97.Chaponnier, C. and Gabbiani, G. (2004). Pathological situations characterized by altered actin isoform expression. J Pathol. 204(4), 386-95.Driesen, R.B., Verheyen F.K., Debie, W., Blaauw, E., Babiker, F.A., Cornelussen, R.N., Ausma, J., Lenders, M.H., Borgers, M., Chaponnier, C. and Ramaekers, F.C. (2009). Re-expression of alpha skeletal actin as a marker for dedifferentiation in cardiac pathologies. J Cell Mol Med 13(5), 896-908.
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 Research Articles on ACTA1    1. These findings suggest that the direct contact of Dengue virus 2 E protein with 43 kDa actin protein may have a crucial function in Dengue virus 2 infection of ECV304 cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-ACTA1 antibodyPathways associated with anti-ACTA1 antibody
 Reference Product  PubMed Publications
 TPM3 antibody  >26 publications with ACTA1 and TPM3
 ACTC1 antibody  >1 publications with ACTA1 and ACTC1
 Products by Pathway  Pathway Diagram
 Caspase Cascade In Apoptosis Pathway antibodies  Caspase Cascade In Apoptosis Pathway Diagram
 Hypothetical Network For Drug Addiction Pathway antibodies  Hypothetical Network For Drug Addiction Pathway Diagram
 Myometrial Relaxation And Contraction Pathways antibodies  Myometrial Relaxation And Contraction Pathways Diagram
 RhoA Signaling Pathway antibodies  RhoA Signaling Pathway Diagram
 Signaling Events Mediated By Focal Adhesion Kinase Pathway antibodies  Signaling Events Mediated By Focal Adhesion Kinase Pathway Diagram
 Striated Muscle Contraction Pathway antibodies  Striated Muscle Contraction Pathway Diagram
Diseases associated with anti-ACTA1 antibodyOrgans/Tissues associated with anti-ACTA1 antibody
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >78 publications with ACTA1 and Nervous System Diseases
 Nemaline myopathy 3 Antibodies  >16 publications with ACTA1 and Nemaline myopathy 3
 Hypertrophy Antibodies  >14 publications with ACTA1 and Hypertrophy
 Heart Diseases Antibodies  >14 publications with ACTA1 and Heart Diseases
 Cardiovascular Diseases Antibodies  >12 publications with ACTA1 and Cardiovascular Diseases
 Atrophy Antibodies  >7 publications with ACTA1 and Atrophy
 Fibrosis Antibodies  >6 publications with ACTA1 and Fibrosis
 Fetal Death Antibodies  >3 publications with ACTA1 and Fetal Death
 Hepatomegaly Antibodies  >2 publications with ACTA1 and Hepatomegaly
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >109 publications with ACTA1 and Muscle
 Heart Antibodies  >22 publications with ACTA1 and Heart
 Brain Antibodies  >9 publications with ACTA1 and Brain
 Embryonic Tissue Antibodies  >6 publications with ACTA1 and Embryonic Tissue
 Bone Antibodies  >4 publications with ACTA1 and Bone
 Nerve Antibodies  >3 publications with ACTA1 and Nerve
 Lung Antibodies  >3 publications with ACTA1 and Lung
 Eye Antibodies  >2 publications with ACTA1 and Eye
 Kidney Antibodies  >2 publications with ACTA1 and Kidney
 Liver Antibodies  >2 publications with ACTA1 and Liver
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