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anti-CRYAA antibody :: Mouse alpha A Crystallin Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS800827
Testing Data
Unit / Price
0.2 mg  /  $450 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

alpha A Crystallin (CRYAA), Monoclonal Antibody

 Also Known As   

Alpha A Crystallin Antibody: ATTO 390

 Product Synonym Names    Heat shock protein beta4; Acry 1; CRYA1; CRYAA; HspB4
 Product Gene Name   

anti-CRYAA antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    gene 604219
 3D Structure    ModBase 3D Structure for P02489
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 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    1H3.B8
 Host    Mouse
 Species Reactivity    Human, Mouse, Cow, Rat
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 Form/Format    Protein G Purified
 Concentration    1mg/mL (lot specific)
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 Conjugate    ATTO 390
 Storage Buffer    PBS pH7.2, in 50% glycerol, 0.09% sodium azide
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 Preparation and Storage    -20 degree C
 Other Notes    Small volumes of anti-CRYAA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-CRYAA antibody

   Background Info: Detects alpha-crystallin at ~20kDa. Does not cross-react with alphaB-crystallin, betaL-crystallin, 'H- crystallin, gamma-crystallin, Hsp25, Hsp27 or Hsp47 proteins.

Scientific Background: The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with Hsp25 and Hsp27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its' phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8).
 Product Categories/Family for anti-CRYAA antibody    Chaperones, Heat Shock, Trafficking
 Applications Tested/Suitable for anti-CRYAA antibody   

Western Blot (WB), ELISA (EIA)

 Application Notes for anti-CRYAA antibody    1:2000 (WB)
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 Testing Data of anti-CRYAA antibody    Western blot analysis of AlphaA Crystallin in bovine tissues at a 1:1000 dilution This blot shows absolute specificity as Left: AlphaA Crystallin, Right: AlphaB Crystallin.
anti-CRYAA antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for CRYAA. It may not necessarily be applicable to this product.
 NCBI GI #    4503055
 NCBI GeneID    1409
 NCBI Accession #    NP_000385.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000394.3 [Other Products]
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 UniProt Primary Accession #    P02489 [Other Products]
 UniProt Secondary Accession #    Q53X53 [Other Products]
 UniProt Related Accession #    P02489; AAB33370 [Other Products]
 Molecular Weight    19,909 Da [Similar Products]
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 NCBI Official Full Name    alpha-crystallin A chain
 NCBI Official Synonym Full Names    crystallin, alpha A
 NCBI Official Symbol    CRYAA [Similar Products]
 NCBI Official Synonym Symbols   
CRYA1; HSPB4; CTRCT9
[Similar Products]
 NCBI Protein Information    alpha-crystallin A chain; crystallin, alpha-1; heat shock protein beta-4; human alphaA-crystallin (CRYA1)
 UniProt Protein Name    Alpha-crystallin A chain
 UniProt Synonym Protein Names   
Heat shock protein beta-4; HspB4
 Protein Family    Alpha-crystallin
 UniProt Gene Name    CRYAA [Similar Products]
 UniProt Synonym Gene Names    CRYA1; HSPB4; HspB4 [Similar Products]
 UniProt Entry Name    CRYAA_HUMAN
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 NCBI Summary for CRYAA    Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC). [provided by RefSeq, Jan 2014]
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 UniProt Comments for CRYAA    CRYA1: a major structural protein of the eye lens. A member of the small heat shock protein (sHSP, also known as the HSP20) family. Alpha-A is preferentially restricted to the lens.

Protein type: Heat shock protein; Chaperone

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: cytoplasm; nucleus

Molecular Function: identical protein binding; protein binding; metal ion binding; unfolded protein binding; structural constituent of eye lens

Biological Process: visual perception; negative regulation of intracellular transport; response to stimulus; protein refolding; protein homooligomerization; negative regulation of apoptosis

Disease: Cataract 9, Multiple Types
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Product References and Citations for anti-CRYAA antibody

   1. Merck K.B. et al. (1993) J Biol Chem. 268: 1046-1052. 2. Horwitz J. (1992) Proc Natl Acad Sci USA 89(21): 10449-10453. 3. Cobb B.A. and Petrash J.M. (2002) Biochemistry. 41: 483-490 4. Horwitz J. (2003) Exp Eye Res. 76: 145-153. 5. Bullard B. et al. (2004) J Biol Chem. 279: 7917-7924. 6. Gangalum R.K., Schibler M.J. and Bhat S.P. (2004) J Biol Chem. 279: 43374.43377. 7. Maddala R. and Rao V.P. (2005) Exp Cell Res. 306: 203-215. 8. Yaung J., et al. (2007) Molecular Vision 13: 566-577.
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 Research Articles on CRYAA    1. This study identified a novel disease-causing mutation c.161 G > T (p.R54L) in CRYAA in a Chinese family with autosomal dominant nuclear cataracts.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-CRYAA antibodyPathways associated with anti-CRYAA antibody
 Reference Product  PubMed Publications
 CRYAB antibody  >22 publications with CRYAA and CRYAB
 CRYGC antibody  >22 publications with CRYAA and CRYGC
 CRYBB2 antibody  >21 publications with CRYAA and CRYBB2
 GJA8 antibody  >16 publications with CRYAA and GJA8
 CRYBB1 antibody  >15 publications with CRYAA and CRYBB1
 CRYBA1 antibody  >14 publications with CRYAA and CRYBA1
 CRYGS antibody  >11 publications with CRYAA and CRYGS
 MIP antibody  >8 publications with CRYAA and MIP
 BFSP2 antibody  >5 publications with CRYAA and BFSP2
 Products by Pathway  Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram
Diseases associated with anti-CRYAA antibodyOrgans/Tissues associated with anti-CRYAA antibody
 Disease Name  Pubmed Publications
 Cataract Antibodies  >67 publications with CRYAA and Cataract
 Nervous System Diseases Antibodies  >6 publications with CRYAA and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >3 publications with CRYAA and Cardiovascular Diseases
 Heart Diseases Antibodies  >2 publications with CRYAA and Heart Diseases
 Inflammation Antibodies  >2 publications with CRYAA and Inflammation
 Edema Antibodies  >1 publications with CRYAA and Edema
 Brain Diseases Antibodies  >1 publications with CRYAA and Brain Diseases
 Mitochondrial Diseases Antibodies  >1 publications with CRYAA and Mitochondrial Diseases
 Liver Diseases Antibodies  >1 publications with CRYAA and Liver Diseases
 Autoimmune Diseases Antibodies  >1 publications with CRYAA and Autoimmune Diseases
 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >61 publications with CRYAA and Eye
 Brain Antibodies  >4 publications with CRYAA and Brain
 Liver Antibodies  >1 publications with CRYAA and Liver
 Thymus Antibodies  >1 publications with CRYAA and Thymus
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