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anti-AP2A1 antibody :: Mouse anti-Human, Rat alpha-Adaptin Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS175087 anti-AP2A1 antibody
Unit / Price
0.1 mg  /  $245 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

alpha-Adaptin (AP2A1), Monoclonal Antibody

 Also Known As   

Anti-alpha-Adaptin antibody (monoclonal)

 Product Synonym Names    AP-2 complex subunit alpha-1; adaptor-related protein complex 2, alpha 1 subunit; ADTAA; CLAPA1; AP2-ALPHA
 Product Gene Name   

anti-AP2A1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    601026
 3D Structure    ModBase 3D Structure for O95782
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 Clonality    Monoclonal
 Isotype    IgG2a
 Clone Number    Ada-1
 Host    Mouse
 Species Reactivity    Human, Rat
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 Purity/Purification    Ascites
 Form/Format    Lyophilized
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 Immunogen    AP-2 adaptor polypeptides from bovine brain.
 Contents    Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
 Reconstitution    1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.
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 Preparation and Storage    At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of anti-AP2A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-AP2A1 antibody

   Description: Mouse IgG monoclonal antibody for alpha-Adaptin, adaptor-related protein complex 2, alpha 1 subunit (AP2A1) detection. Tested with WB in Human, rat. No cross reactivity with other proteins. Uniprot ID: O95782
Background: Alpha Adaptin, also known as adaptor related protein complex, alpha 1 subunit. The alpha adaptins are exclusively found in the endocytic coated vesicles. Huntingtin-interacting protein 1(HIP1) is enriched in membrane-containing cell fractions and has been implicated in vesicle trafficking. Waelter et al.(2001) identified 3 HIP1-associated proteins, clathrin heavy chain(CLTC)and alpha-adaptin A and C. Coat proteins of approximately 100-kD(adaptins) are components of the adaptor complexes which link clathrin to receptors in coated vesicles. The alpha-adaptins separate into two bands on SDS gels.
 Applications Tested/Suitable for anti-AP2A1 antibody   

Western Blot (WB)

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NCBI/Uniprot data below describe general gene information for AP2A1. It may not necessarily be applicable to this product.
 NCBI GI #    262527580
 NCBI GeneID    160
 NCBI Accession #    O95782.3 [Other Products]
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 UniProt Primary Accession #    O95782 [Other Products]
 UniProt Secondary Accession #    Q96CI7; Q96PP6; Q96PP7; Q9H070 [Other Products]
 UniProt Related Accession #    O95782 [Other Products]
 Molecular Weight    105,361 Da
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 NCBI Official Full Name    AP-2 complex subunit alpha-1
 NCBI Official Synonym Full Names    adaptor-related protein complex 2, alpha 1 subunit
 NCBI Official Symbol    AP2A1 [Similar Products]
 NCBI Official Synonym Symbols   
ADTAA; CLAPA1; AP2-ALPHA
[Similar Products]
 NCBI Protein Information    AP-2 complex subunit alpha-1; alpha1-adaptin; alpha-adaptin A; adaptin, alpha A; 100 kDa coated vesicle protein A; adaptor protein complex AP-2 subunit alpha-1; adapter-related protein complex 2 alpha-1 subunit; adapter-related protein complex 2 subunit alpha-1; adaptor-related protein complex 2 subunit alpha-1; clathrin assembly protein complex 2 alpha-A large chain; plasma membrane adaptor HA2/AP2 adaptin alpha A subunit; clathrin-associated/assembly/adaptor protein, large, alpha 1
 UniProt Protein Name    AP-2 complex subunit alpha-1
 UniProt Synonym Protein Names   
100 kDa coated vesicle protein A; Adaptor protein complex AP-2 subunit alpha-1; Adaptor-related protein complex 2 subunit alpha-1; Alpha-adaptin A; Alpha1-adaptin; Clathrin assembly protein complex 2 alpha-A large chain; Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit
 Protein Family    AP-2 complex
 UniProt Gene Name    AP2A1 [Similar Products]
 UniProt Synonym Gene Names    ADTAA; CLAPA1 [Similar Products]
 UniProt Entry Name    AP2A1_HUMAN
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 NCBI Summary for AP2A1    This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined. [provided by RefSeq, Jul 2008]
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 UniProt Comments for AP2A1    AP2A1: Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin- coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C- terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif. Belongs to the adaptor complexes large subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Vesicle

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: clathrin coat of trans-Golgi network vesicle; membrane; basolateral plasma membrane; apical plasma membrane; plasma membrane; AP-2 adaptor complex; cytosol

Molecular Function: protein C-terminus binding; protein binding; protein kinase binding; protein transporter activity

Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; epidermal growth factor receptor signaling pathway; axon guidance; synaptic transmission; intracellular protein transport; viral reproduction; nerve growth factor receptor signaling pathway; ephrin receptor signaling pathway; Golgi to endosome transport; antigen processing and presentation of exogenous peptide antigen via MHC class II; endocytosis; regulation of defense response to virus by virus
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 Research Articles on AP2A1    1. A protein encoded by this locus was found to be differentially expressed in postmortem brains from patients with atypical frontotemporal lobar degeneration.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-AP2A1 antibodyPathways associated with anti-AP2A1 antibody
 Reference Product  PubMed Publications
 CLTC antibody  >1 publications with AP2A1 and CLTC
 NUMB antibody  >1 publications with AP2A1 and NUMB
 EPS15 antibody  >1 publications with AP2A1 and EPS15
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Arf1 Pathway antibodies  Arf1 Pathway Diagram
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 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
 EPH-Ephrin Signaling Pathway antibodies  EPH-Ephrin Signaling Pathway Diagram
 EPH-ephrin Mediated Repulsion Of Cells Pathway antibodies  EPH-ephrin Mediated Repulsion Of Cells Pathway Diagram
 Endocrine And Other Factor-regulated Calcium Reabsorption Pathway antibodies  Endocrine And Other Factor-regulated Calcium Reabsorption Pathway Diagram
Diseases associated with anti-AP2A1 antibodyOrgans/Tissues associated with anti-AP2A1 antibody
 Disease Name  Pubmed Publications
 Gonadal Disorders Antibodies  >1 publications with AP2A1 and Gonadal Disorders
 Ovarian Diseases Antibodies  >1 publications with AP2A1 and Ovarian Diseases
 Weight Gain Antibodies  >1 publications with AP2A1 and Weight Gain
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >1 publications with AP2A1 and Muscle
 Blood Antibodies  >1 publications with AP2A1 and Blood
 Testis Antibodies  >1 publications with AP2A1 and Testis
 Brain Antibodies  >1 publications with AP2A1 and Brain
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