View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Add To Compare List Mouse g-Catenin (C-terminal) Monoclonal Antibody Catalog # MBS470014 Unit / Price DISCONTINUEDYou will be redirected to our replacement product Previous Next Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name g-Catenin (C-terminal), Monoclonal Antibody Popular Item Full Product Name Anti-gamma-Catenin (C-terminal) Product Synonym Names g-Catenin (C-terminal); Junction Plakoglobin; JUP; Desmoplakin III; Desmoplakin-3 Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Monoclonal Isotype IgG2a Host Mouse Species Reactivity Human, Rat, Mouse Specificity The antibody detects an 84kDa* protein corresponding to the molecular mass of gamma-Catenin on SDS-PAGE immunoblots of A431 and Hct116 src transformed cells. In addition, this antibody recognizes only gamma-Catenin in immunoprecipitations using anti-gamma-Catenin versus anti- beta -Catenin. Purity/Purification Protein A Purified Form/Format Mouse monoclonal antibody purified with protein A chromatography is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Immunogen Clone was generated from a recombinant protein that includes amino acid residues in the C-terminal region of rat gamma-Catenin. This peptide sequence is highly conserved in human and mouse gamma-Catenin. Preparation and Storage Store at -20°C. Stable for 1 year. Other Notes Small volumes of anti-g-Catenin (C-terminal) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for anti-g-Catenin (C-terminal) antibody Plakoglobin (gamma-Catenin) is a catenin family member identified as a component of desmosomes. gamma -Catenin has high homology to beta-catenin and, like beta-catenin, it can associate with the cadherins, E-cadherin and N-cadherin. One molecule of alpha-catenin and at least one molecule of beta-catenin and gamma -Catenin simultaneously bind to a single cadherin molecule. A 19-amino acid sequence of desmoglein was found to be critical for binding of gamma -Catenin. Similar catenin-binding domains found in cadherins, suggest a common mechanism for gamma -Catenin localization to both adherens junctions and desmosomes. Phosphorylation of tyrosine residues in gamma -Catenin can modify its interactions with other proteins. Phosphorylation of tyrosine 644 decreases gamma -Catenin association with alpha-catenin, but increases binding to desmoplakin. Fer kinase can phosphorylate tyrosine 550, which increases gamma -Catenin binding to ?-catenin. Thus, tyrosine phosphorylation may be important for regulation of gamma -Catenin protein-protein interactions within desmosomal complexes. Applications Tested/Suitable for anti-g-Catenin (C-terminal) antibody ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry Application Notes for anti-g-Catenin (C-terminal) antibody ELISA: 1:2000IP: 1:100WB: 1:1000ICC: 1:50End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature. Testing Data of anti-g-Catenin (C-terminal) antibody Western blot analysis of anti-gamma-Catenin (C-terminal) immunoprecipitates from pervanadate-treated A431. The immunoprecipitates were untreated (lanes 1,3,7) or treated with alkaline phosphatase (lanes 2,4,8). The blots were probed with gamma-Catenin (a.a. 545-555), gamna-Catenin (Tyr-550) or gamma-Catenin (C-terminal) antibodies. The anti-gamma-Catenin (Tyr-550) was used in the presence of gamma-Catenin (Tyr-550) (lane 5) or gamma-Catenin (Tyr-644) (lane 6) peptides. IHC of anti-g-Catenin (C-terminal) antibody Immunocytochemical labeling of phosphorylated gamma-Catenin in control (Top) and pervanadate-treated (Bottom) A431 cells. The cells were co-labeled with mouse monoclonal gamma-Catenin or rabbit polyclonal gamma-Catenin (Tyr-550) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy2 or Cy3. TOP Product References and Citations for anti-g-Catenin (C-terminal) antibody • McCrea, P.D. et al. (1991) Science 254:1359.• Miravet, S. et al. (2003) Mol. Cell. Biol. 23(20) :7391. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP