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anti-SILV antibody :: Mouse gp100 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS438452
Testing Data
Unit / Price
0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $150 +1 FREE 8GB USB
0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $270 +1 FREE 8GB USB
0.1 mg (Without BSA & Azide at 1mg/ml)  /  $285 +1 FREE 8GB USB
0.2 mg (With BSA & Azide at 0.2mg/ml)  /  $360 +1 FREE 8GB USB
0.2 mg (Without BSA & Azide at 1mg/ml)  /  $380 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

gp100 (SILV), Monoclonal Antibody

 Also Known As   

gp100/ Melanosome/ PMEL17/ SILV

 Product Synonym Names    95kDa melanocyte-specific secreted glycoprotein; M-beta; Melanocyte lineage specific antigen GP100; Melanocyte protein Pmel 17; Melanoma associated ME20 antigen; Melanosomal matrix protein17; p100; p26; PMEL17; Premelanosome protein; Secreted melanoma-associated ME20 antigen; SILV; Silver homolog
 Product Gene Name   

anti-SILV antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    155550
 3D Structure    ModBase 3D Structure for P40967
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 Clonality    Monoclonal
 Isotype    IgG2b, kappa
 Clone Number    NKI-beteb
 Host    Mouse
 Species Reactivity    Human, Horse.Does not react with rat, Others-not tested.
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 Specificity    By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
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 Localization    Cytoplasmic
 Positive Control    SK-MEL-28 cells or Melanoma
 Human Chromosomal Location    12q13.2
 Immunogen    Membranes from a human melanoma metastasis
 Quantity and Buffer    0.5 ml at 200ug/ml with BSA and azide
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 Preparation and Storage    Store at 2 to 8 degree C. Antibody is stable for 24 months.
 Other Notes    Small volumes of anti-SILV antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-SILV antibody   

ELISA (EIA), Flow Cytometry(FC/FACS, Immunofluorescence (IF), Immunohistology(IH)

 Application Notes for anti-SILV antibody    ELISA (For coating, order Ab without BSA); Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistology (Frozen & Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.
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 Testing Data of anti-SILV antibody    Formalin-paraffin human melanoma tissue stained with gp100 Ab (NKI-beteb),
anti-SILV antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for SILV. It may not necessarily be applicable to this product.
 NCBI GI #    318037593
 NCBI GeneID    6490
 NCBI Accession #    NP_001186982.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001200053.1 [Other Products]
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 UniProt Primary Accession #    P40967 [Other Products]
 UniProt Secondary Accession #    Q12763; Q14448; Q14817; Q16565; B3KS57; B7Z6D7 [Other Products]
 UniProt Related Accession #    P40967 [Other Products]
 Molecular Weight    90-100kDa
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 NCBI Official Full Name    melanocyte protein PMEL isoform 2
 NCBI Official Synonym Full Names    premelanosome protein
 NCBI Official Symbol    PMEL [Similar Products]
 NCBI Official Synonym Symbols   
P1; SI; SIL; ME20; P100; SILV; ME20M; gp100; ME20-M; PMEL17; D12S53E
[Similar Products]
 NCBI Protein Information    melanocyte protein PMEL; melanocyte protein mel 17; silver, mouse, homolog of; melanocyte protein Pmel 17; melanosomal matrix protein17; silver locus protein homolog; melanoma-associated ME20 antigen; melanocytes lineage-specific antigen GP100
 UniProt Protein Name    Melanocyte protein PMEL
 UniProt Synonym Protein Names   
ME20-M; ME20M; Melanocyte protein Pmel 17; Melanocytes lineage-specific antigen GP100; Melanoma-associated ME20 antigen; P1; P100; Premelanosome protein; Silver locus protein homologCleaved into the following 2 chains:M-alphaAlternative name(s):95 kDa melanocyte-specific secreted glycoprotein; P26; Secreted melanoma-associated ME20 antigen; ME20-S; ME20S
 UniProt Gene Name    PMEL [Similar Products]
 UniProt Synonym Gene Names    D12S53E; PMEL17; SILV; ME20M; ME20-S; ME20S [Similar Products]
 UniProt Entry Name    PMEL_HUMAN
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 NCBI Summary for SILV    This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2011]
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 UniProt Comments for SILV    SILV: Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity. Belongs to the PMEL/NMB family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Vesicle

Chromosomal Location of Human Ortholog: 12q13-q14

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; melanosome; plasma membrane; extracellular region; integral to membrane

Molecular Function: protein binding

Biological Process: melanin biosynthetic process; melanosome organization and biogenesis
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Product References and Citations for anti-SILV antibody

   1. Vennegoor C. et al., Am.J. Pathol. 130: 179-192, 1988.
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 Research Articles on SILV    1. SIL-TAL1 rearrangement identifies a distinct subtype with inferior outcome which could allow for individual therapeutic stratification for T-ALL patients.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with anti-SILV antibodyOrgans/Tissues associated with anti-SILV antibody
 Disease Name  Pubmed Publications
 Skin Diseases Antibodies  >114 publications with SILV and Skin Diseases
 Disease Models, Animal Antibodies  >81 publications with SILV and Disease Models, Animal
 Necrosis Antibodies  >17 publications with SILV and Necrosis
 Nervous System Diseases Antibodies  >16 publications with SILV and Nervous System Diseases
 Lung Diseases Antibodies  >15 publications with SILV and Lung Diseases
 Brain Diseases Antibodies  >9 publications with SILV and Brain Diseases
 Leukopenia Antibodies  >6 publications with SILV and Leukopenia
 Pain Antibodies  >5 publications with SILV and Pain
 Kidney Diseases Antibodies  >5 publications with SILV and Kidney Diseases
 Drug Hypersensitivity Antibodies  >4 publications with SILV and Drug Hypersensitivity
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >126 publications with SILV and Skin
 Lung Antibodies  >22 publications with SILV and Lung
 Eye Antibodies  >19 publications with SILV and Eye
 Spleen Antibodies  >18 publications with SILV and Spleen
 Brain Antibodies  >15 publications with SILV and Brain
 Kidney Antibodies  >12 publications with SILV and Kidney
 Bone Antibodies  >10 publications with SILV and Bone
 Testis Antibodies  >8 publications with SILV and Testis
 Liver Antibodies  >8 publications with SILV and Liver
 Muscle Antibodies  >7 publications with SILV and Muscle
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