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anti-hENT1 antibody :: Rabbit anti-Human hENT1 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS303184
Immunohistochemistry (IHC)
Unit / Price
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 Product Name   

hENT1, Monoclonal Antibody

★Popular Item★
 Also Known As   

Rabbit Anti-Human hENT1 Monoclonal Antibody (Clone SP120)

 Product Gene Name   

anti-hENT1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Monoclonal
 Isotype    IgG
 Clone Number    SP120
 Host    Rabbit
 Species Reactivity    Human
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 Purity/Purification    Purified from TCS by protein A/G.
 Form/Format    Liquid
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 Immunogen    Synthetic peptide (internal sequence). The exact sequence is propietary.
 Positive Control    Pancreas,tissue, Human tonsil tissue
 Buffer    pH: 7:60
Preservative : 0.01% Sodium azide
Constituents: 98% PBS, 1% BSA
 Function    Mediates both influx and effiux of nucleosides across the membrane (equilibrateive transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine, inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs).
 Tissue specifity    Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen.
 Sequence similarities    Belongs to the SLC29A transporter family.
 Post -transactional modifications    Glycosylated.
 Cellular localization    Basolateral cell, membrane . Apical cell membrane. Predominantly localized in the basolateral membrane in polarised MDCK cells.
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 Preparation and Storage    Shipped at 4C . Store at 4C short term (1-2 weeks). Upon delivery aliquot. Store at -20C long term/ Avoid freeze/thaw cycles.
 Other Notes    Small volumes of anti-hENT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-hENT1 antibody

   Human equilibrative nucleoside transporter 1 (hENT1) is a member of the equilibrative nucleoside transporter family. It is a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is sensitive to inhibition by nitrobenzylthioinosine (NBMPR). It is considered as a drug carrier. Nucleoside transporters such as hENT1 are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies.
 Applications Tested/Suitable for anti-hENT1 antibody   


 Application Notes for anti-hENT1 antibody    Use at an assay dependen concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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 Immunohistochemistry (IHC) of anti-hENT1 antibody    Immunohistochemical analysis of formalin-fixed paraffin-embedded Human pancreas tissue labeling ENT1 with ab182023
anti-hENT1 antibody Immunohistochemistry (IHC) (IHC) image
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 Molecular Weight    50 kDa
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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