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anti-p53 antibody :: Mouse anti-Human p53 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS190547 anti-p53 antibody
Unit / Price
0.1 mg  /  $340 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

p53, Monoclonal Antibody

 Also Known As   

Anti-p53 Monoclonal Antibody

 Product Gene Name   

anti-p53 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence Positions    46-53
 Chromosome Location    Chromosome: 17; NC_000017.10 (7571720..7590863, complement). Location: 17p13.1
 OMIM    133239
 3D Structure    ModBase 3D Structure for P04637
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 Clonality    Monoclonal
 Isotype    lgG1
 Host    Mouse
 Species Reactivity    Human
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 Specificity    p53
This antibody recognizes human p53.
 Form/Format    100ug in PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein A affinity chromatography.
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 Antigen    Fusion protein corresponding to aa 46-53 of human p53.
 Dilution Instructions    Dilute in PBS or medium which is identical to that used in the assay system.
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 Preparation and Storage    This antibody is stable for at least one (1) year at -20 degree C. Avoid repeated freezing and thawing.
 Other Notes    Small volumes of anti-p53 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-p53 antibody

   p53 is a DNA-binding, cell cycle-regulating transcription factor that governs cell division. It plays a critical role in tumor suppression and has been referred to as "the guardian angel gene" or "the master watchman". Defects in p53 are linked to >50% of human cancers and restoring p53 function to cancer cells can induce growth arrest and apoptosis. Damaged p53 can also lead to autoimmune disorders.
 Applications Tested/Suitable for anti-p53 antibody   

ELISA; Immunoblot; Immunohistochemistry; Immunoprecipitation; Immunoprecipitation

 Application Notes for anti-p53 antibody    ELISA, Immunoblotting (1:1,000-1:2,000), Immunohistochemistry (1:100-1:250), Immunoprecipitation (10ug/mg).
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NCBI/Uniprot data below describe general gene information for p53. It may not necessarily be applicable to this product.
 NCBI GI #    23491729
 NCBI GeneID    7157
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 UniProt Primary Accession #    P04637 [Other Products]
 UniProt Secondary Accession #    Q15086; Q15087; Q15088; Q16535; Q16807; Q16808; Q16809; Q16810; Q16811; Q16848; Q2XN98 [Other Products]
 UniProt Related Accession #    P04637; Q0PKT5; Q0ZAJ9; Q0ZAK0; Q0ZAK1; Q19RW5; Q19RW6; Q1HGV0; Q1HGV1; Q1HGV2 [Other Products]
 Molecular Weight    43,653 Da
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 NCBI Official Full Name    P53
 NCBI Official Synonym Full Names    tumor protein p53
 NCBI Official Symbol    TP53 [Similar Products]
 NCBI Official Synonym Symbols   
P53; LFS1; TRP53; FLJ92943
[Similar Products]
 NCBI Protein Information    cellular tumor antigen p53; antigen NY-CO-13; OTTHUMP00000221333; OTTHUMP00000221334; OTTHUMP00000221336; OTTHUMP00000221337; OTTHUMP00000221340; phosphoprotein p53; p53 tumor suppressor; transformation-related protein 53
 UniProt Protein Name    Cellular tumor antigen p53
 UniProt Synonym Protein Names   
Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53
 Protein Family    p53 and DNA damage-regulated protein
 UniProt Gene Name    TP53 [Similar Products]
 UniProt Synonym Gene Names    P53 [Similar Products]
 UniProt Entry Name    P53_HUMAN
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 NCBI Summary for p53    This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity. [provided by RefSeq]
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 UniProt Comments for p53    p53: a transcription factor and major tumor suppressor that plays a major role in regulating cellular responses to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. More than 50 percent of human tumors contain a mutation or deletion of the TP53 gene. p53 is modified post-translationally at multiple sites. DNA damage induces phosphorylation of p53 at S15, S20 and S37, reducing its interaction with the oncoprotein MDM2. MDM2 inhibits p53 accumulation by targeting it for ubiquitination and proteasomal degradation. Phosphorylated by many kinases including Chk2 and Chk1 at S20, enhancing its tetramerization, stability and activity. The phosphorylation by CAK at S392 is increased in human tumors and has been reported to influence the growth suppressor function, DNA binding and transcriptional activation of p53. Phosphorylation of p53 at S46 regulates the ability of p53 to induce apoptosis. The acetylation of p53 appears to play a positive role in the accumulation of p53 during the stress response. Following DNA damage, p53 becomes acetylated at K382, enhancing its binding to DNA. Deacetylation of p53 can occur through interaction with SIRT1, a deacetylase that may be involved in cellular aging and the DNA damage response. p53 regulates the transcription of a set of genes encoding endosomal proteins that regulate endosomal functions. These include STEAP3 and CHMP4C, which enhance exosome production, and CAV1 and CHMP4C, which produce a more rapid endosomal clearance of the EGFR from the plasma membrane. DNA damage regulates a p53-mediated secretory pathway, increasing the secretion of some proteins such as Hsp90, SERPINE1, SERPINB5, NKEF-A, and CyPA, and inhibiting the secretion of others including CTSL and IGFBP-2. Two alternatively spliced human isoforms have been reported. Isoform 2 is expressed in quiescent lymphocytes. Seems to be non-functional. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Protein type: DNA-binding; Tumor suppressor; Nuclear receptor co-regulator; Motility/polarity/chemotaxis; Activator; Transcription factor

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: PML body; transcription factor TFIID complex; protein complex; nuclear matrix; mitochondrion; endoplasmic reticulum; replication fork; cytosol; nucleoplasm; nuclear body; mitochondrial matrix; nuclear chromatin; cytoplasm; nucleolus; chromatin; nucleus

Molecular Function: identical protein binding; protease binding; protein phosphatase 2A binding; zinc ion binding; p53 binding; protein N-terminus binding; receptor tyrosine kinase binding; protein kinase binding; protein phosphatase binding; transcription factor binding; histone acetyltransferase binding; protein binding; histone deacetylase regulator activity; enzyme binding; copper ion binding; DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; chaperone binding; damaged DNA binding; chromatin binding; transcription factor activity; ATP binding

Biological Process: viral reproduction; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; multicellular organismal development; T cell differentiation in the thymus; gastrulation; determination of adult life span; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; response to antibiotic; regulation of apoptosis; cellular response to glucose starvation; protein localization; negative regulation of neuroblast proliferation; base-excision repair; transforming growth factor beta receptor signaling pathway; cerebellum development; protein complex assembly; cell cycle arrest; ER overload response; response to X-ray; somitogenesis; release of cytochrome c from mitochondria; chromatin assembly; cell aging; circadian behavior; rRNA transcription; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of fibroblast proliferation; negative regulation of DNA replication; embryonic organ development; positive regulation of transcription from RNA polymerase II promoter; regulation of mitochondrial membrane permeability; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis; regulation of tissue remodeling; transcription from RNA polymerase II promoter; G1 DNA damage checkpoint; DNA damage response, signal transduction by p53 class mediator; apoptosis; negative regulation of transcription from RNA polymerase II promoter; response to salt stress; entrainment of circadian clock by photoperiod; negative regulation of cell proliferation; positive regulation of protein oligomerization; positive regulation of histone deacetylation; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; regulation of transcription, DNA-dependent; T cell proliferation during immune response; positive regulation of neuron apoptosis; double-strand break repair; response to gamma radiation; cell differentiation; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; protein tetramerization; mitochondrial DNA repair; Notch signaling pathway; in utero embryonic development; multicellular organism growth; B cell lineage commitment; cell proliferation; neuron apoptosis; T cell lineage commitment; negative regulation of helicase activity; nucleotide-excision repair; protein import into nucleus, translocation; DNA strand renaturation; Ras protein signal transduction; negative regulation of cell growth; negative regulation of transforming growth factor beta receptor signaling pathway; blood coagulation; response to DNA damage stimulus

Disease: Papilloma Of Choroid Plexus; Pancreatic Cancer; Nasopharyngeal Carcinoma; Breast Cancer; Li-fraumeni Syndrome 1; Osteogenic Sarcoma; Colorectal Cancer; Adrenocortical Carcinoma, Hereditary; Glioma Susceptibility 1; Hepatocellular Carcinoma; Basal Cell Carcinoma, Susceptibility To, 7
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 Research Articles on p53    1. Data show that the CIS surrounding morphologically normal urothelium with a high frequency of abnormal DNA content, clear aneuploid cells, p53 mutated protein expression, and a proliferative status underlying a field carcinogenesis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-p53 antibodyPathways associated with anti-p53 antibody
 Reference Product  PubMed Publications
 MDM2 antibody  >541 publications with p53 and MDM2
 CDKN1A antibody  >430 publications with p53 and CDKN1A
 BRCA1 antibody  >283 publications with p53 and BRCA1
 BAX antibody  >260 publications with p53 and BAX
 ATM antibody  >223 publications with p53 and ATM
 BRCA2 antibody  >206 publications with p53 and BRCA2
 PTGS2 antibody  >56 publications with p53 and PTGS2
 BCR antibody  >53 publications with p53 and BCR
 CHEK2 antibody  >47 publications with p53 and CHEK2
 BCL2L1 antibody  >44 publications with p53 and BCL2L1
 Products by Pathway  Pathway Diagram
 Activation Of BH3-only Proteins Pathway antibodies  Activation Of BH3-only Proteins Pathway Diagram
 Activation Of NOXA And Translocation To Mitochondria Pathway antibodies  Activation Of NOXA And Translocation To Mitochondria Pathway Diagram
 Activation Of PUMA And Translocation To Mitochondria Pathway antibodies  Activation Of PUMA And Translocation To Mitochondria Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
Diseases associated with anti-p53 antibodyOrgans/Tissues associated with anti-p53 antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >4043 publications with p53 and Neoplasms
 Li-Fraumeni Syndrome Antibodies  >2197 publications with p53 and Li-Fraumeni Syndrome
 Adenocarcinoma Antibodies  >685 publications with p53 and Adenocarcinoma
 Breast Neoplasms Antibodies  >638 publications with p53 and Breast Neoplasms
 Intestinal Neoplasms Antibodies  >454 publications with p53 and Intestinal Neoplasms
 Colorectal Neoplasms Antibodies  >452 publications with p53 and Colorectal Neoplasms
 Leukemia Antibodies  >417 publications with p53 and Leukemia
 Carcinoma, Squamous Cell Antibodies  >393 publications with p53 and Carcinoma, Squamous Cell
 Head and Neck Neoplasms Antibodies  >366 publications with p53 and Head and Neck Neoplasms
 Lung Neoplasms Antibodies  >355 publications with p53 and Lung Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >481 publications with p53 and Blood
 Lung Antibodies  >418 publications with p53 and Lung
 Brain Antibodies  >356 publications with p53 and Brain
 Bladder Antibodies  >211 publications with p53 and Bladder
 Liver Antibodies  >197 publications with p53 and Liver
 Skin Antibodies  >181 publications with p53 and Skin
 Lymph Node Antibodies  >174 publications with p53 and Lymph Node
 Bone Antibodies  >169 publications with p53 and Bone
 Lymph Antibodies  >168 publications with p53 and Lymph
 Prostate Antibodies  >126 publications with p53 and Prostate
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