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anti-p53 antibody :: Mouse p53 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS212267 anti-p53 antibody
Unit / Price
0.1 mg  /  $425 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

p53, Monoclonal Antibody

★Popular Item★
 Also Known As   

MOUSE ANTI HUMAN p53 (aa181-190)

 Product Gene Name   

anti-p53 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    133239
 3D Structure    ModBase 3D Structure for P04637
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 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    DO-11
 Host    Mouse
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 Form/Format    Purified
Purified IgG - liquid
 Concentration    IgG concentration 1.0 mg/ml (lot specific)
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 Perservative Stabilisers    0.09% Sodium Azide
 Histology Positive Control Tissue    Colon or breast carcinoma
Buffer Solution
 Target Species    Human
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 Preparation and Storage    Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
 Other Notes    Small volumes of anti-p53 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-p53 antibody

   Mouse anti Human p53 antibody, clone DO-11 recognizes the human Cellular tumor antigen p53, also known as p53 tumour suppressor protein or NY-CO-13. p53 is a 393 amino acid ~53kDa cytoplasmic/ nuclear protein up-regulated in response to DNA damage and is found in a wide variety of transformed cells (UniProt: P04637). DO-11 binds to an epitope within the central region of p53 between amino acids 181 - 190 defining a cryptic epitope exposed in unfolded/ denatured p53 (Shimitzu et al. 2006). Nine isoforms of human p53 are produced by alternative splicing and promotor usage, the epitope recognized by clone DO-11 is present in all isoforms. Mouse anti Human p53 antibody, clone DO-11 recognizes mutant forms of p53 (Warnock et al. 2011) and has been used successfully for detection of p53 by western blotting (Wei et al. 2012).
 Applications Tested/Suitable for anti-p53 antibody   

Immunohistology Frozen, Immunoprecipitation (IP), Immunohistology Paraffin*, Western Blot (WB)

 Application Notes for anti-p53 antibody    Immunohistology - Paraffin: Maximum Dilution: 1/1000; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blotting: Maximum Dilution: 1/1000
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NCBI/Uniprot data below describe general gene information for p53. It may not necessarily be applicable to this product.
 NCBI GI #    120407068
 NCBI GeneID    7157
 NCBI Accession #    NP_000537.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_000546.5 [Other Products]
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 UniProt Primary Accession #    P04637 [Other Products]
 UniProt Secondary Accession #    Q15086; Q15087; Q15088; Q16535; Q16807; Q16808; Q16809; Q16810; Q16811; Q16848; Q2XN98 [Other Products]
 UniProt Related Accession #    P04637 [Other Products]
 Molecular Weight    24,401 Da
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 NCBI Official Full Name    cellular tumor antigen p53 isoform a
 NCBI Official Synonym Full Names    tumor protein p53
 NCBI Official Symbol    TP53 [Similar Products]
 NCBI Official Synonym Symbols   
P53; BCC7; LFS1; TRP53
[Similar Products]
 NCBI Protein Information    cellular tumor antigen p53; antigen NY-CO-13; mutant tumor protein 53; p53 tumor suppressor; phosphoprotein p53; transformation-related protein 53
 UniProt Protein Name    Cellular tumor antigen p53
 UniProt Synonym Protein Names   
Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53
 Protein Family    p53 and DNA damage-regulated protein
 UniProt Gene Name    TP53 [Similar Products]
 UniProt Synonym Gene Names    P53 [Similar Products]
 UniProt Entry Name    P53_HUMAN
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 NCBI Summary for p53    This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013]
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 UniProt Comments for p53    p53: a transcription factor and major tumor suppressor that plays a major role in regulating cellular responses to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. More than 50 percent of human tumors contain a mutation or deletion of the TP53 gene. p53 is modified post-translationally at multiple sites. DNA damage induces phosphorylation of p53 at S15, S20 and S37, reducing its interaction with the oncoprotein MDM2. MDM2 inhibits p53 accumulation by targeting it for ubiquitination and proteasomal degradation. Phosphorylated by many kinases including Chk2 and Chk1 at S20, enhancing its tetramerization, stability and activity. The phosphorylation by CAK at S392 is increased in human tumors and has been reported to influence the growth suppressor function, DNA binding and transcriptional activation of p53. Phosphorylation of p53 at S46 regulates the ability of p53 to induce apoptosis. The acetylation of p53 appears to play a positive role in the accumulation of p53 during the stress response. Following DNA damage, p53 becomes acetylated at K382, enhancing its binding to DNA. Deacetylation of p53 can occur through interaction with SIRT1, a deacetylase that may be involved in cellular aging and the DNA damage response. p53 regulates the transcription of a set of genes encoding endosomal proteins that regulate endosomal functions. These include STEAP3 and CHMP4C, which enhance exosome production, and CAV1 and CHMP4C, which produce a more rapid endosomal clearance of the EGFR from the plasma membrane. DNA damage regulates a p53-mediated secretory pathway, increasing the secretion of some proteins such as Hsp90, SERPINE1, SERPINB5, NKEF-A, and CyPA, and inhibiting the secretion of others including CTSL and IGFBP-2. Two alternatively spliced human isoforms have been reported. Isoform 2 is expressed in quiescent lymphocytes. Seems to be non-functional. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Protein type: Nuclear receptor co-regulator; Motility/polarity/chemotaxis; Tumor suppressor; Transcription factor; Activator; DNA-binding

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: PML body; transcription factor TFIID complex; nuclear matrix; protein complex; mitochondrion; endoplasmic reticulum; replication fork; cytosol; nucleoplasm; nuclear body; mitochondrial matrix; nuclear chromatin; cytoplasm; nucleolus; nucleus; chromatin

Molecular Function: identical protein binding; protease binding; zinc ion binding; protein phosphatase 2A binding; p53 binding; protein N-terminus binding; receptor tyrosine kinase binding; transcription factor binding; protein kinase binding; protein phosphatase binding; histone acetyltransferase binding; protein binding; copper ion binding; enzyme binding; histone deacetylase regulator activity; DNA binding; protein heterodimerization activity; chaperone binding; ubiquitin protein ligase binding; damaged DNA binding; chromatin binding; transcription factor activity; ATP binding

Biological Process: viral reproduction; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; multicellular organismal development; T cell differentiation in the thymus; gastrulation; determination of adult life span; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; response to antibiotic; cellular response to glucose starvation; protein localization; negative regulation of neuroblast proliferation; base-excision repair; transforming growth factor beta receptor signaling pathway; cerebellum development; protein complex assembly; cell cycle arrest; ER overload response; response to X-ray; somitogenesis; release of cytochrome c from mitochondria; chromatin assembly; cell aging; circadian behavior; rRNA transcription; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of DNA replication; negative regulation of fibroblast proliferation; embryonic organ development; positive regulation of transcription from RNA polymerase II promoter; regulation of mitochondrial membrane permeability; negative regulation of transcription, DNA-dependent; regulation of tissue remodeling; negative regulation of apoptosis; G1 DNA damage checkpoint; transcription from RNA polymerase II promoter; DNA damage response, signal transduction by p53 class mediator; apoptosis; negative regulation of transcription from RNA polymerase II promoter; response to salt stress; entrainment of circadian clock by photoperiod; positive regulation of protein oligomerization; negative regulation of cell proliferation; positive regulation of histone deacetylation; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; regulation of transcription, DNA-dependent; T cell proliferation during immune response; double-strand break repair; positive regulation of neuron apoptosis; response to gamma radiation; cell differentiation; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; protein tetramerization; mitochondrial DNA repair; Notch signaling pathway; in utero embryonic development; B cell lineage commitment; multicellular organism growth; cell proliferation; neuron apoptosis; T cell lineage commitment; negative regulation of helicase activity; nucleotide-excision repair; protein import into nucleus, translocation; DNA strand renaturation; Ras protein signal transduction; negative regulation of cell growth; blood coagulation; negative regulation of transforming growth factor beta receptor signaling pathway; response to DNA damage stimulus

Disease: Papilloma Of Choroid Plexus; Pancreatic Cancer; Nasopharyngeal Carcinoma; Breast Cancer; Li-fraumeni Syndrome 1; Osteogenic Sarcoma; Colorectal Cancer; Glioma Susceptibility 1; Adrenocortical Carcinoma, Hereditary; Basal Cell Carcinoma, Susceptibility To, 7; Hepatocellular Carcinoma
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 Research Articles on p53    1. Decreased miR-34a expression and increased FOXP1, p53, and BCL2 coexpression predict poor overall survival for MALT lymphoma and diffuse large B-cell lymphoma patients
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-p53 antibodyDiseases associated with anti-p53 antibody
 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Activation Of BH3-only Proteins Pathway antibodies  Activation Of BH3-only Proteins Pathway Diagram
 Activation Of NOXA And Translocation To Mitochondria Pathway antibodies  Activation Of NOXA And Translocation To Mitochondria Pathway Diagram
 Activation Of PUMA And Translocation To Mitochondria Pathway antibodies  Activation Of PUMA And Translocation To Mitochondria Pathway Diagram
 Alzheimers Disease Pathway antibodies  Alzheimers Disease Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >2846 publications with p53 and Carcinoma
 Breast Neoplasms Antibodies  >1078 publications with p53 and Breast Neoplasms
 Colorectal Neoplasms Antibodies  >720 publications with p53 and Colorectal Neoplasms
 Carcinoma, Squamous Cell Antibodies  >684 publications with p53 and Carcinoma, Squamous Cell
 Glioma Antibodies  >583 publications with p53 and Glioma
 Carcinoma, Small Cell Antibodies  >413 publications with p53 and Carcinoma, Small Cell
 Neoplasm Metastasis Antibodies  >406 publications with p53 and Neoplasm Metastasis
 Cell Transformation, Neoplastic Antibodies  >404 publications with p53 and Cell Transformation, Neoplastic
 Sarcoma Antibodies  >346 publications with p53 and Sarcoma
 Liver Neoplasms Antibodies  >344 publications with p53 and Liver Neoplasms
Organs/Tissues associated with anti-p53 antibody
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >919 publications with p53 and Blood
 Lung Antibodies  >781 publications with p53 and Lung
 Brain Antibodies  >626 publications with p53 and Brain
 Liver Antibodies  >434 publications with p53 and Liver
 Bone Antibodies  >329 publications with p53 and Bone
 Skin Antibodies  >316 publications with p53 and Skin
 Lymph Node Antibodies  >312 publications with p53 and Lymph Node
 Lymph Antibodies  >304 publications with p53 and Lymph
 Bladder Antibodies  >298 publications with p53 and Bladder
 Prostate Antibodies  >228 publications with p53 and Prostate
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