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anti-p63 antibody :: Rabbit anti-Human p63 Monoclonal Antibody

Scan QR to view Datasheet Catalog #    MBS684293
Unit / Price
1 ml (Prediluted)  /  $175 +1 FREE 8GB USB
0.04 mL  /  $185 +1 FREE 8GB USB
0.1 mL  /  $345 +1 FREE 8GB USB
7 ml (Prediluted)  /  $370 +1 FREE 8GB USB
0.2 mL  /  $470 +1 FREE 8GB USB
15 ml (Prediluted)  /  $545 +1 FREE 8GB USB
0.5 mL  /  $590 +1 FREE 8GB USB
1 mL  /  $905 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

p63, Monoclonal Antibody

★Popular Item★
 Also Known As   


 Product Gene Name   

anti-p63 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    103285
 3D Structure    ModBase 3D Structure for Q9H3D4
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 Clonality    Monoclonal
 Clone Number    V22-E
 Host    Rabbit
 Species Reactivity    Human
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 Buffer    20 mM Tris-HCl, pH 8.0
 Stabilizer    20 mg/ml BSA
 Preservative    0.05% NaN3
 Immunogen    Peptide derived from the internal sequence of human p63. Antibody recognizes the epitope between Tyr231 - Arg251.
 Cellular localization    nucleus
 Positive control    tonsil tissue
1. Deparaffinize the section in 3 changes of xylene, 10 minutes each.
2. Wash the section in 96%, 80% and 70% ethyl alcohol for 10 minutes each.
3. Rinse in distilled water, 2 x 5 minutes.
4. Block the endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes.
5. Wash in distilled water, 2 x 5 minutes.
6. For antigen retrieval: Immerse the slide in Tris-EDTA buffer*, pH 9.0 and incubate at 95-97C in water bath for 25 minutes.
7. Remove the staining to room temperature and let the slide to cool (in Tris-EDTA buffer, pH 9.0) for 15 minutes.
8. Rinse in distilled water, 2 x 5 minutes.
9. Wash in PBS (phosphate buffer saline, pH 7.0-7.5) supplemented with 0.05% of Tween-20 (buffer A), 2 x 5 minutes.

Incubate the section with primary antibody at the dilution 1:100 - 1:200 for 1 hour in the closed wet chamber.
Incubate the section with primary antibody (ready to use) for 1 hour in a closed wet chamber.
11. Wash 3 x 5 minutes with buffer A.
12. Apply the secondary antibody (the protocol depends on the supplier), and proceed3to standard immunohistochemistry protocol (HRP - Peroxide - DAB). Micropolymer-HRP detection kit rabbit/mouse dual is suggested
13. Wash 3 x 5 minutes with buffer A.
14. Apply the chromogen (DAB), 1 - 3 minutes.
15. Wash in water, 2 x 5 minutes.
16. Stain in hematoxylin for 5 minutes.
17. Wash in distilled water, 3 x 2 minutes.
18. Mount the slide for observation.

1. Deparafinization (Enter).
2. Heating glass (72oC) with the medium temperatures. Deparafinization.
3. Prolonged deparafinization (Enter).
4. Cell conditioning (Enter).
5. ULTRA Conditioner #2 (Enter).
6. Heating glass (97C), incubation 8 min (Cell conditioner #2).
7. ULTRA CC2 solution application 24 min (Enter).
8. Titration (Enter).
9. Hand apply primary antibody. Incubation 32 min.
10. UltraWash (Enter).
11. Nuclear stain (Enter).
12. Hematoxylin application one drop (nuclear stain). Cover and incubate 8 min.
13. After nuclear stain (Enter).
14. Bluing reagent application, one drop. After nuclear stain, cover and incubate 4 min.
 PRECAUTIONS    1. Intended for professional In Vitro Diagnostic use in laboratories.
2. Do not use after expiration date stamped on vial label.
3. Avoid contamination of the reagent.
4. Any discrepancies in the recommended procedures stated in the working protocol may affect the final results.
5. The reagent contains sodium azide (NaN3) which is highly toxic in higher concentrations. The concentration in the reagent (0.05%) is not considered as hazardous.
6. Disposal of waste material must be conducted in accordance with local regulations.
7. Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin.
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 Preparation and Storage    Storage: +4 degree C
 ISO Certification    Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
 Other Notes    Small volumes of anti-p63 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-p63 antibody   

Immunohistochemistry (IHC) Paraffin

 Application Notes for anti-p63 antibody    Immunohistochemistry (IHC) Paraffin: 1:100 - 1:200
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 Immunohistochemistry (IHC) of anti-p63 antibody    anti-p63 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-p63 antibody    anti-p63 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for p63. It may not necessarily be applicable to this product.
 NCBI GI #    169234657
 NCBI GeneID    8626
 NCBI Accession #    NP_001108450.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001114978.1 [Other Products]
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 UniProt Primary Accession #    Q9H3D4 [Other Products]
 UniProt Secondary Accession #    O75080; O75195; O75922; O76078; Q6VEG2; Q6VEG3; Q6VEG4; Q6VFJ1; Q6VFJ2; Q6VFJ3; Q6VH20 [Other Products]
 UniProt Related Accession #    Q9H3D4 [Other Products]
 Molecular Weight    65,288 Da
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 NCBI Official Full Name    tumor protein 63 isoform 2
 NCBI Official Synonym Full Names    tumor protein p63
 NCBI Official Symbol    TP63 [Similar Products]
 NCBI Official Synonym Symbols   
AIS; KET; LMS; NBP; RHS; p40; p51; p63; EEC3; OFC8; p73H; p73L; SHFM4; TP53L; TP73L; p53CP; TP53CP; B(p51A); B(p51B)
[Similar Products]
 NCBI Protein Information    tumor protein 63
 UniProt Protein Name    Tumor protein 63
 UniProt Synonym Protein Names   
Chronic ulcerative stomatitis protein; CUSP; Keratinocyte transcription factor KET; Transformation-related protein 63; TP63; Tumor protein p73-like; p73L; p40; p51
 UniProt Gene Name    TP63 [Similar Products]
 UniProt Synonym Gene Names    KET; P63; P73H; P73L; TP73L; p63; CUSP; TP63; p73L [Similar Products]
 UniProt Entry Name    P63_HUMAN
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 NCBI Summary for p63    This gene encodes a member of the p53 family of transcription factors. An animal model, p63 -/- mice, has been useful in defining the role this protein plays in the development and maintenance of stratified epithelial tissues. p63 -/- mice have several developmental defects which include the lack of limbs and other tissues, such as teeth and mammary glands, which develop as a result of interactions between mesenchyme and epithelium. Mutations in this gene are associated with ectodermal dysplasia, and cleft lip/palate syndrome 3 (EEC3); split-hand/foot malformation 4 (SHFM4); ankyloblepharon-ectodermal defects-cleft lip/palate; ADULT syndrome (acro-dermato-ungual-lacrimal-tooth); limb-mammary syndrome; Rap-Hodgkin syndrome (RHS); and orofacial cleft 8. Both alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different proteins. Many transcripts encoding different proteins have been reported but the biological validity and the full-length nature of these variants have not been determined. [provided by RefSeq, Jul 2008]
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 UniProt Comments for p63    p63: Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. Isoform 2 activates RIPK4 transcription. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge. Activates transcription of the p21 promoter. Binds DNA as a homotetramer. Isoform composition of the tetramer may determine transactivation activity. Isoforms Alpha and Gamma interact with HIPK2. Interacts with SSRP1, leading to stimulate coactivator activity. Isoform 1 and isoform 2 interact with WWP1. Interacts with PDS5A. Isoform 5 (via activation domain) interacts with NOC2L. Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues. Belongs to the p53 family. 12 isoforms of the human protein are produced by alternative promoter.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 3q28

Cellular Component: cytoplasm; cytosol; dendrite; nuclear chromatin; nucleoplasm; nucleus; rough endoplasmic reticulum; transcription factor complex

Molecular Function: chromatin binding; damaged DNA binding; DNA binding; double-stranded DNA binding; identical protein binding; metal ion binding; p53 binding; protein binding; sequence-specific DNA binding; transcription factor activity; WW domain binding

Biological Process: apoptosis; chromatin remodeling; cloacal septation; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; ectoderm and mesoderm interaction; embryonic limb morphogenesis; epidermal cell division; epithelial cell development; establishment of planar polarity; female genitalia morphogenesis; G1 DNA damage checkpoint; gene expression; hair follicle morphogenesis; keratinocyte differentiation; keratinocyte proliferation; multicellular organismal aging; negative regulation of apoptosis; negative regulation of keratinocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; neuron apoptosis; Notch signaling pathway; odontogenesis of dentine-containing teeth; polarized epithelial cell differentiation; positive regulation of mesenchymal cell proliferation; positive regulation of Notch signaling pathway; positive regulation of osteoblast differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein homotetramerization; proximal/distal pattern formation; regulation of caspase activity; regulation of epidermal cell division; regulation of neuron apoptosis; replicative cell aging; response to DNA damage stimulus; response to gamma radiation; response to X-ray; skeletal development; skin morphogenesis; smooth muscle development; spermatogenesis; sympathetic nervous system development; transcription initiation from RNA polymerase II promoter; urinary bladder development

Disease: Adult Syndrome; Ankyloblepharon-ectodermal Defects-cleft Lip/palate; Ectrodactyly, Ectodermal Dysplasia, And Cleft Lip/palate Syndrome 3; Limb-mammary Syndrome; Rapp-hodgkin Syndrome; Split-hand/foot Malformation 4
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 Research Articles on p63    1. Delta Np63alpha-regulated microRNAs were found to regulate the levels of several metabolic factors
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-p63 antibodyPathways associated with anti-p63 antibody
 Reference Product  PubMed Publications
 TP53 antibody  >128 publications with p63 and TP53
 MDM2 antibody  >51 publications with p63 and MDM2
 TP73 antibody  >22 publications with p63 and TP73
 IRF6 antibody  >7 publications with p63 and IRF6
 ITCH antibody  >6 publications with p63 and ITCH
 PPP1R13L antibody  >3 publications with p63 and PPP1R13L
 UBC antibody  >3 publications with p63 and UBC
 SATB2 antibody  >1 publications with p63 and SATB2
 Products by Pathway  Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 TP53 Regulates Metabolic Genes Pathway antibodies  TP53 Regulates Metabolic Genes Pathway Diagram
 TP53 Network Pathway antibodies  TP53 Network Pathway Diagram
 Transcriptional Regulation By TP53 Pathway antibodies  Transcriptional Regulation By TP53 Pathway Diagram
Diseases associated with anti-p63 antibodyOrgans/Tissues associated with anti-p63 antibody
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >475 publications with p63 and Carcinoma
 Neoplasms, Squamous Cell Antibodies  >298 publications with p63 and Neoplasms, Squamous Cell
 Carcinoma, Squamous Cell Antibodies  >280 publications with p63 and Carcinoma, Squamous Cell
 Adenocarcinoma Antibodies  >181 publications with p63 and Adenocarcinoma
 Abnormalities, Multiple Antibodies  >129 publications with p63 and Abnormalities, Multiple
 Skin Abnormalities Antibodies  >108 publications with p63 and Skin Abnormalities
 Lung Neoplasms Antibodies  >105 publications with p63 and Lung Neoplasms
 Cleft Palate Antibodies  >95 publications with p63 and Cleft Palate
 Female Urogenital Diseases Antibodies  >94 publications with p63 and Female Urogenital Diseases
 Cleft Lip Antibodies  >93 publications with p63 and Cleft Lip
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >207 publications with p63 and Skin
 Lung Antibodies  >129 publications with p63 and Lung
 Eye Antibodies  >90 publications with p63 and Eye
 Prostate Antibodies  >72 publications with p63 and Prostate
 Mouth Antibodies  >69 publications with p63 and Mouth
 Embryonic Tissue Antibodies  >52 publications with p63 and Embryonic Tissue
 Lymph Antibodies  >45 publications with p63 and Lymph
 Bladder Antibodies  >43 publications with p63 and Bladder
 Muscle Antibodies  >43 publications with p63 and Muscle
 Thyroid Antibodies  >36 publications with p63 and Thyroid
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