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anti-MLC-2 antibody :: Myosin Light Chain 2 Antibody

Scan QR to view Datasheet Catalog #    MBS857334
Unit / Price
0.1 mg  /  $215 +1 FREE 8GB USB
0.05 mg  /  $225 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Myosin Light Chain 2 (MLC-2), Antibody

★Popular Item★
 Also Known As   

Myosin Light Chain 2 (Phospho-Ser18) Antibody

 Product Gene Name   

anti-MLC-2 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    600899
 3D Structure    ModBase 3D Structure for P78527
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 Species Reactivity    Human, Mouse, Rat
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 Specificity    Myosin Light Chain 2 (Phospho-Ser18) Antibody detects endogenous levels of Myosin LightChain 2 only when phosphorylated at serine 18.
 Purity/Purification    Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
 Form/Format    In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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 Immunogen    The antiserum was produced against synthesized phosphopeptide derived from human Myosin Light Chain 2 around the phosphorylation site of serine 18 (A-T-SP-N-V).
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 Preparation and Storage    Store at-20 degree C for 1 year
 Other Notes    Small volumes of anti-MLC-2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for anti-MLC-2 antibody    Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells
 Applications Tested/Suitable for anti-MLC-2 antibody   

Western Blot (WB), Immunohistochemistry (IHC)

 Application Notes for anti-MLC-2 antibody    WB: 1:500~1:1000
IHC: 1:50~1:100
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 Testing Data of anti-MLC-2 antibody    P-Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Myosin Light Chain 2
(Phospho-Ser18) Antibody (E011114).
anti-MLC-2 antibody Testing Data image
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 Testing Data #2 of anti-MLC-2 antibody    Western blot analysis of extracts from mouse heart tissue
using Myosin Light Chain 2 (Phospho-Ser18) Antibody
(E011114).
anti-MLC-2 antibody Testing Data #2 image
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NCBI/Uniprot data below describe general gene information for MLC-2. It may not necessarily be applicable to this product.
 NCBI GI #    126032350
 NCBI GeneID    5591
 NCBI Accession #    NP_001075109.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001081640.1 [Other Products]
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 UniProt Primary Accession #    P78527 [Other Products]
 UniProt Secondary Accession #    P78528; Q13327; Q13337; Q14175; Q59H99; Q7Z611; Q96SE6; Q9UME3 [Other Products]
 UniProt Related Accession #    P78527 [Other Products]
 Molecular Weight    465,501 Da
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 NCBI Official Full Name    DNA-dependent protein kinase catalytic subunit isoform 2
 NCBI Official Synonym Full Names    protein kinase, DNA-activated, catalytic polypeptide
 NCBI Official Symbol    PRKDC [Similar Products]
 NCBI Official Synonym Symbols   
HYRC; p350; DNAPK; DNPK1; HYRC1; IMD26; XRCC7; DNA-PKcs
[Similar Products]
 NCBI Protein Information    DNA-dependent protein kinase catalytic subunit
 UniProt Protein Name    DNA-dependent protein kinase catalytic subunit
 UniProt Synonym Protein Names   
DNPK1; p460
 Protein Family    Myosin
 UniProt Gene Name    PRKDC [Similar Products]
 UniProt Synonym Gene Names    HYRC; HYRC1; DNA-PK catalytic subunit; DNA-PKcs [Similar Products]
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 NCBI Summary for MLC-2    This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.[provided by RefSeq, Jul 2010]
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 UniProt Comments for MLC-2    Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely through an indirect mechanism. Interacts with CRY1 and CRY2; negatively regulates CRY1 phosphorylation. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.
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Product References and Citations for anti-MLC-2 antibody

   Janiak A, et al. (2006) Mol Biol Cell. Apr; 17(4): 1606-1619. Croft DR, et al. (2006) Mol Cell Biol. 2 Jun; 26(12): 4612-4627 Li Z, et al. (2006) Mol Cell Biol. Jun; 26(11): 4240-4256.
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 Research Articles on MLC-2    1. The crystal structure defines a stage on which many of the components assemble and regulate the kinase activity through modulating the conformation and allosteric regulation of kinase activity.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-MLC-2 antibodyPathways associated with anti-MLC-2 antibody
 Reference Product  PubMed Publications
 ATM antibody  >248 publications with MLC-2 and ATM
 XRCC5 antibody  >210 publications with MLC-2 and XRCC5
 JUN antibody  >126 publications with MLC-2 and JUN
 XRCC4 antibody  >98 publications with MLC-2 and XRCC4
 DCLRE1C antibody  >37 publications with MLC-2 and DCLRE1C
 CASP3 antibody  >36 publications with MLC-2 and CASP3
 PARP1 antibody  >27 publications with MLC-2 and PARP1
 RPA1 antibody  >22 publications with MLC-2 and RPA1
 NBN antibody  >15 publications with MLC-2 and NBN
 TP53 antibody  >14 publications with MLC-2 and TP53
 Products by Pathway  Pathway Diagram
 BARD1 Signaling Events Pathway antibodies  BARD1 Signaling Events Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Class I PI3K Signaling Events Mediated By Akt Pathway antibodies  Class I PI3K Signaling Events Mediated By Akt Pathway Diagram
 Coregulation Of Androgen Receptor Activity Pathway antibodies  Coregulation Of Androgen Receptor Activity Pathway Diagram
 Cytosolic Sensors Of Pathogen-associated DNA Pathway antibodies  Cytosolic Sensors Of Pathogen-associated DNA Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 DNA-PK Complex Pathway antibodies  DNA-PK Complex Pathway Diagram
 Double-Strand Break Repair Pathway antibodies  Double-Strand Break Repair Pathway Diagram
Diseases associated with anti-MLC-2 antibodyOrgans/Tissues associated with anti-MLC-2 antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >358 publications with MLC-2 and Neoplasms
 Chromosome Aberrations Antibodies  >76 publications with MLC-2 and Chromosome Aberrations
 Nervous System Diseases Antibodies  >68 publications with MLC-2 and Nervous System Diseases
 Breast Neoplasms Antibodies  >44 publications with MLC-2 and Breast Neoplasms
 Drug Toxicity Antibodies  >40 publications with MLC-2 and Drug Toxicity
 Lymphoma Antibodies  >37 publications with MLC-2 and Lymphoma
 Neoplasms, Experimental Antibodies  >31 publications with MLC-2 and Neoplasms, Experimental
 Lung Neoplasms Antibodies  >30 publications with MLC-2 and Lung Neoplasms
 Lung Diseases Antibodies  >30 publications with MLC-2 and Lung Diseases
 Head and Neck Neoplasms Antibodies  >22 publications with MLC-2 and Head and Neck Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >163 publications with MLC-2 and Blood
 Lung Antibodies  >51 publications with MLC-2 and Lung
 Brain Antibodies  >50 publications with MLC-2 and Brain
 Bone Antibodies  >37 publications with MLC-2 and Bone
 Bone Marrow Antibodies  >33 publications with MLC-2 and Bone Marrow
 Liver Antibodies  >32 publications with MLC-2 and Liver
 Spleen Antibodies  >26 publications with MLC-2 and Spleen
 Ovary Antibodies  >22 publications with MLC-2 and Ovary
 Thymus Antibodies  >22 publications with MLC-2 and Thymus
 Kidney Antibodies  >21 publications with MLC-2 and Kidney
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