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anti-POLR2A antibody :: POLR2A Antibody

Scan QR to view Datasheet Catalog #    MBS8502228
Unit / Price
0.1 mg  /  $215 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

POLR2A, Antibody

★Popular Item★
 Also Known As   

POLR2A (Ab-1619) Antibody

 Product Synonym Names    RPO2; RPOL2; POLR2 ALPHA-AMANITIN RESISTANCE; POLYMERASE II RNA; SUBUNIT A; RNA POLYMERASE II; 220-KD SUBUNIT; RNA POLYMERASE II; LARGE SUBUNIT; RPB1; S. CEREVISIAE; HOMOLOG OF polymerase (RNA) II (DNA directed) polypeptide A
 Product Gene Name   

anti-POLR2A antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    180660
 3D Structure    ModBase 3D Structure for P24928
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 Species Reactivity    Human, Mouse, Rat
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 Specificity    POLR2A (Ab-1619) antibody detects endogenous levels of total POLR2A protein.
 Purity/Purification    Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
 Form/Format    In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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 Immunogen    The antiserum was produced against synthesized non-phosphopeptide derived from human POLR2A around the phosphorylation site of serine 1619 (P-T-S-P-S).
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 Preparation and Storage    Store at-20 degree C for 1 year
 Other Notes    Small volumes of anti-POLR2A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for anti-POLR2A antibody    Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells
 Applications Tested/Suitable for anti-POLR2A antibody   

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)

 Application Notes for anti-POLR2A antibody    WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:10000
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 Immunohistochemistry (IHC) of anti-POLR2A antibody    Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using POLR2A (Ab-1619) antibody.
anti-POLR2A antibody Immunohistochemistry (IHC) (IHC) image
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 Western Blot (WB) of anti-POLR2A antibody    Western blot analysis of extracts from COS7 cells treated with EGF (200ng/ml, 30min), using POLR2A (Ab-1619) antibody.
anti-POLR2A antibody Western Blot (WB) (WB) image
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 Immunofluorescence (IF) of anti-POLR2A antibody    PMA +- Immunofluorescence analysis of HeLa cells, treated with PMA (125ng/ml, 30mins), using POLR2A (Ab-1619) antibody.
anti-POLR2A antibody Immunofluorescence (IF) image
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NCBI/Uniprot data below describe general gene information for POLR2A. It may not necessarily be applicable to this product.
 NCBI GI #    4505939
 NCBI GeneID    5430
 NCBI Accession #    NP_000928.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000937.4 [Other Products]
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 UniProt Primary Accession #    P24928 [Other Products]
 UniProt Secondary Accession #    Q6NX41; A6NN93; B9EH88 [Other Products]
 UniProt Related Accession #    P24928 [Other Products]
 Molecular Weight    63,641 Da
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 NCBI Official Full Name    DNA-directed RNA polymerase II subunit RPB1
 NCBI Official Synonym Full Names    RNA polymerase II subunit A
 NCBI Official Symbol    POLR2A [Similar Products]
 NCBI Official Synonym Symbols   
RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220
[Similar Products]
 NCBI Protein Information    DNA-directed RNA polymerase II subunit RPB1
 UniProt Protein Name    DNA-directed RNA polymerase II subunit RPB1
 UniProt Synonym Protein Names   
DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA-directed RNA polymerase II subunit RPB1 (EC:2.7.7.48)
 UniProt Gene Name    POLR2A [Similar Products]
 UniProt Synonym Gene Names    POLR2; RNA polymerase II subunit B1 [Similar Products]
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 NCBI Summary for POLR2A    This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq, Jul 2008]
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 UniProt Comments for POLR2A    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Regulation of gene expression levels depends on the balance between methylation and acetylation levels of tha CTD-lysines (). Initiation or early elongation steps of transcription of growth-factors-induced immediate early genes are regulated by the acetylation status of the CTD (PubMed:24207025). Methylation and dimethylation have a repressive effect on target genes expression ().
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Product References and Citations for anti-POLR2A antibody

   Tracy Jo Pasieka, J. Virol., Aug 2006; 80: 7600-7612. Olaf R. Mook, Mol. Cancer Ther., Mar 2007; 6: 833-843. Colleen A. McMullen, J. Neurosci., Jan 2004; 24: 161.
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 Research Articles on POLR2A    1. Results identified rs2071504 in POLR2A gene to be associated with poor overall and disease-free survival of patients with an early-stage non-small cell lung cancer.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-POLR2A antibodyPathways associated with anti-POLR2A antibody
 Reference Product  PubMed Publications
 ACTB antibody  >11 publications with POLR2A and ACTB
 MYC antibody  >2 publications with POLR2A and MYC
 ABL1 antibody  >1 publications with POLR2A and ABL1
 NRIP1 antibody  >1 publications with POLR2A and NRIP1
 CDK9 antibody  >1 publications with POLR2A and CDK9
 CCND1 antibody  >1 publications with POLR2A and CCND1
 Products by Pathway  Pathway Diagram
 Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway antibodies  Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Dual Incision Reaction In TC-NER Pathway antibodies  Dual Incision Reaction In TC-NER Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Eukaryotic Transcription Initiation Pathway antibodies  Eukaryotic Transcription Initiation Pathway Diagram
 Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway antibodies  Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway Diagram
 Formation Of HIV-1 Elongation Complex In The Absence Of HIV-1 Tat Pathway antibodies  Formation Of HIV-1 Elongation Complex In The Absence Of HIV-1 Tat Pathway Diagram
 Formation Of RNA Pol II Elongation Complex Pathway antibodies  Formation Of RNA Pol II Elongation Complex Pathway Diagram
Diseases associated with anti-POLR2A antibodyOrgans/Tissues associated with anti-POLR2A antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >14 publications with POLR2A and Neoplasms
 Cardiovascular Diseases Antibodies  >4 publications with POLR2A and Cardiovascular Diseases
 Adenocarcinoma Antibodies  >4 publications with POLR2A and Adenocarcinoma
 Disease Models, Animal Antibodies  >3 publications with POLR2A and Disease Models, Animal
 Adenoma Antibodies  >2 publications with POLR2A and Adenoma
 Colonic Neoplasms Antibodies  >1 publications with POLR2A and Colonic Neoplasms
 Stomach Neoplasms Antibodies  >1 publications with POLR2A and Stomach Neoplasms
 Poisoning Antibodies  >1 publications with POLR2A and Poisoning
 Drug Toxicity Antibodies  >1 publications with POLR2A and Drug Toxicity
 Inflammation Antibodies  >1 publications with POLR2A and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >7 publications with POLR2A and Brain
 Heart Antibodies  >5 publications with POLR2A and Heart
 Liver Antibodies  >4 publications with POLR2A and Liver
 Lung Antibodies  >4 publications with POLR2A and Lung
 Kidney Antibodies  >3 publications with POLR2A and Kidney
 Muscle Antibodies  >3 publications with POLR2A and Muscle
 Adipose Tissue Antibodies  >2 publications with POLR2A and Adipose Tissue
 Connective Tissue Antibodies  >2 publications with POLR2A and Connective Tissue
 Stomach Antibodies  >1 publications with POLR2A and Stomach
 Embryonic Tissue Antibodies  >1 publications with POLR2A and Embryonic Tissue
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