View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Add To Compare List E Coli 12-tag Protein Marker Polyclonal Antibody Catalog # MBS560006 Unit / Price DISCONTINUEDClick here to view our similar product(s) OR click here to contact our technical support team for suitable replacement. Previous Next Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name 12-tag Protein Marker, Polyclonal Antibody Popular Item Full Product Name 12-tag Protein Marker - Positive Control Product Synonym Names 12 Tag Fusion Protein Lysate Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Polyclonal Host E Coli Form/Format 12 epitope tagsPhysical State: Liquid Buffer 1x reduced SDS-PAGE sample loading buffer Procedure Bacteria were lysed with SDS-PAGE sample loading dye, boiled at 95 degree C for 10 minutes, then centrifuged at 13,000 g for 15 minutes. Preparation and Storage 4 degree C for less than 7 days or < or = -20 Deg. C for long term storage.Shelf Life: 12 months after shipment date. Other Notes Small volumes of anti-12-tag Protein Marker antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for anti-12-tag Protein Marker antibody This bacterial lysate contains a 41 kDa protein expressing 12 commonly used tags which are fused in the order of: GST / T7 / HSV / C-MYC / VSV-g / Glu-Glu / V5 / E-tag / DYKDDDDK / S-TAG / HA / 6xHIS. Suggested use is that of a positive control in blotting assays. Product Categories/Family for anti-12-tag Protein Marker antibody Epitope tag Application Notes for anti-12-tag Protein Marker antibody Suggested loading volumes for western blot are 0.25ul - 2ul per lane. This E.coli lysate contains a 41 kd recombinant protein expressing 12 commonly used Tags which are fused in the order of GST - T7 - HSV - CMYC - VSV - Glu-Glu - V5 - E-tag - DYKDDDDK - S-tag - HA - 6xHIS. Recommend loading 0.5ul - 4.0ul cell lysate for 10-12% gels. Testing Data of anti-12-tag Protein Marker antibody Wester Blot of anti-12-tag Protein Marker antibody Wester Blot of 12-tag marker against ICL's affinity purified rabbit anti-HA used at 1:5,000 Lane 1: 2.0ul 12-marker Lane 2 MW Standard TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP