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anti-14-3-3 antibody :: Sheep 14-3-3 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS221344
Testing Data #1
Unit / Price
0.1 mg  /  $455 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

14-3-3, Polyclonal Antibody

 Also Known As   

SHEEP ANTI 14-3-3 (pSer185)

 Product Gene Name   

anti-14-3-3 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Sheep
 Species Reactivity    Bovine, Human, Mammals (Expected from Sequence), Mouse, Rabbit, Rat
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 Specificity    This item recognises the 14-3-3 isoforms beta and zeta which are phosphorylated at serine 185. The members of the 14-3-3 family of proteins are highly conserved and are involved in multiple signalling pathways. The phosphorylated forms of beta and zeta 14-3-3 are found endogenously in brain tissue.
 Form/Format    Purified
Purified IgG - liquid
 Concentration    IgG concentration 1.0mg/ml (lot specific)
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 Perservative Stabilisers    0.09% Sodium Azide (NaN3)
 Immunogen    Synthetic peptide corresponding to 14-3-3 beta and zeta phosphorylated at serine 185.Peptide sequence: EILNSPEKAC
 Antiserum Preparation    Antisera to Phosphorylated 14-3-3 were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
 Target Species    Sheep
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 Preparation and Storage    Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from the date of despatch.
 Other Notes    Small volumes of anti-14-3-3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-14-3-3 antibody

   MBS221344 recognises the 14-3-3 isoforms beta and zeta which are phosphorylated at serine 185. The members of the 14-3-3 family of proteins are highly conserved and are involved in multiple signalling pathways. The phosphorylated forms of beta and zeta 14-3-3 are found endogenously in brain tissue.
 Applications Tested/Suitable for anti-14-3-3 antibody   

Western Blot (WB)*

 Application Notes for anti-14-3-3 antibody    Western Blot: This item detects a band of approximately 30kDa.
Western Blotting: Minimum Dilution: 1/100; Maximum Dilution: 1/300; Application Note: For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
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 Testing Data #1 of anti-14-3-3 antibody    Western blot with Sheep anti-14-3-3 (pSER185) antibody (MBS221344)
Lane 1, 2ug recombinant 14-3-3 beta
Lane 2, 2ug recombinant 14-3-3 zeta
Lane 3, sheep brain homogenate, which contains ca. 50% of beta and zeta isoforms that are phosphorylated on Ser 185
The recombinant 14-3-3s are expressed in E.coli and are not phosphorylated
anti-14-3-3 antibody Testing Data #1 image
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NCBI/Uniprot data below describe general gene information for 14-3-3. It may not necessarily be applicable to this product.
 NCBI GI #    71153782
 NCBI Accession #    P68253.1 [Other Products]
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 UniProt Secondary Accession #    P29359 [Other Products]
 Molecular Weight    18,000 Da
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 NCBI Official Full Name    14-3-3 protein gamma
 UniProt Protein Name    14-3-3 protein gamma
 UniProt Synonym Protein Names   
Protein kinase C inhibitor protein 1; KCIP-1
 Protein Family    14-3-3 protein
 UniProt Gene Name    YWHAG [Similar Products]
 UniProt Synonym Gene Names    KCIP-1 [Similar Products]
 UniProt Entry Name    1433G_SHEEP
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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