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anti-NT5E antibody :: Rabbit anti-Mouse 5'-Nucleotidase, Ecto (NT5E) Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS2002571 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Western Blot (WB)
Unit / Price
0.01 mg  /  $135 +1 FREE 8GB USB
0.05 mg  /  $230 +1 FREE 8GB USB
0.1 mg  /  $310 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

5'-Nucleotidase, Ecto (NT5E), Polyclonal Antibody

 Also Known As   

Polyclonal Antibody to 5'-Nucleotidase, Ecto (NT5E)

 Product Gene Name   

anti-NT5E antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    The target protein is fused with a His-tag and its sequence is listed below.
MGHHHHHHSG SEF-WE LTILHTNDVH SRLEQTSDDS TKCLNASLCV GGVARLFTKV QQIRKEEPNV LFLDAGDQYQ GTIWFTVYKG LEVAHFMNIL GYDAMALGNH EFDNGVEGLI DPLLRNVKFP ILSANIKARG PLAHQISGLF LPSKVLSVGG EVVGIVGYTS KETPFLSNPG TNLVFEDEIS ALQPEVDKLK TLNVNKIIAL GHSGFEMDKL IAQKVRGVDI VVGGHSNTFL YTGNPPSKEV PAGKYPFIVT ADDGRQVPVV QAYAFGKYLG YLKVEFDDKG NVITSYGNPI LLNSSIPEDA TIKADINQWR IKLDNYSTQE LGRTIVYLDG STQTCRFREC NMGNLICDAM INNNLRHPDE MFWNHVSMCI VNGGGIRSPI DEKNNGTITW ENLAAVLPFG GTFDLVQLKG STLKKAFEHS VHRYGQSTGE FLQVGGIHVV YDINRKPWNR VVQLEVLCTK CRVPIYEPLE MDKVYKVTLP SYLANGGDGF QMIKDELLKH DSGDQDISVV SEYISKMKVV YPAVEGRIKF
 Chromosome Location    Chromosome: 6; NC_000006.11 (86159302..86205509). Location: 6q14-q21
 OMIM    129190
 3D Structure    ModBase 3D Structure for P21589
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Mouse
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 Specificity    The antibody is a rabbit polyclonal antibody raised against NT5E. It has been selected for its ability to recognize NT5E in immunohistochemical staining andwestern blotting.
 Purity/Purification    Affinity Chromatography
 Form/Format    Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
 Concentration    200ug/ml (lot specific)
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 Immunogen    Recombinant NT5E (Trp29~phe550) expressed in E.coli.
 Quality Control    Content: The quality control contains recombinant CRP (Phe17~Pro224) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
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 Preparation and Storage    Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
 Other Notes    Small volumes of anti-NT5E antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-NT5E antibody   

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

 Application Notes for anti-NT5E antibody    Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
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 Western Blot (WB) of anti-NT5E antibody    anti-NT5E antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for NT5E. It may not necessarily be applicable to this product.
 NCBI GI #    23897
 NCBI GeneID    4907
 NCBI Accession #    CAA39271.1 [Other Products]
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 UniProt Primary Accession #    P21589 [Other Products]
 UniProt Secondary Accession #    O75520; Q5W116; B3KQI8 [Other Products]
 UniProt Related Accession #    P21589 [Other Products]
 Molecular Weight    63,368 Da
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 NCBI Official Full Name    5'-nucleotidase
 NCBI Official Synonym Full Names    5'-nucleotidase, ecto (CD73)
 NCBI Official Symbol    NT5E [Similar Products]
 NCBI Official Synonym Symbols   
NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
[Similar Products]
 NCBI Protein Information    5'-nucleotidase; 5'-NT; ecto-5'-nucleotidase; Purine 5-Prime-Nucleotidase
 UniProt Protein Name    5'-nucleotidase
 UniProt Synonym Protein Names   
Ecto-5'-nucleotidase
 UniProt Gene Name    NT5E [Similar Products]
 UniProt Synonym Gene Names    NT5; NTE; 5'-NT [Similar Products]
 UniProt Entry Name    5NTD_HUMAN
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 NCBI Summary for NT5E    The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
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 UniProt Comments for NT5E    NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.3.5; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Phosphatase (non-protein); Membrane protein, integral; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 6q14-q21

Cellular Component: cell surface; membrane; cytoplasm; plasma membrane

Molecular Function: 5'-nucleotidase activity; metal ion binding; nucleotide binding

Biological Process: pyrimidine nucleoside catabolic process; pyrimidine base metabolic process; dephosphorylation; nucleobase, nucleoside and nucleotide metabolic process; adenosine biosynthetic process; negative regulation of inflammatory response; purine nucleotide catabolic process; DNA metabolic process; purine base metabolic process; AMP catabolic process

Disease: Calcification Of Joints And Arteries
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 Research Articles on NT5E    1. This study demonstrated that the differential CD73 expression around intestinal tuberculosis granuloma indicates that Mycobacterium tuberculosis evades host immunity by recruiting mesenchymal cells with CD73 expression.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-NT5E antibodyPathways associated with anti-NT5E antibody
 Reference Product  PubMed Publications
 ENPP1 antibody  >4 publications with NT5E and ENPP1
 ADA antibody  >1 publications with NT5E and ADA
 ENTPD1 antibody  >1 publications with NT5E and ENTPD1
 Products by Pathway  Pathway Diagram
 HIF-1-alpha Transcription Factor Network Pathway antibodies  HIF-1-alpha Transcription Factor Network Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Nucleotides Pathway antibodies  Metabolism Of Nucleotides Pathway Diagram
 Nicotinate And Nicotinamide Metabolism Pathway antibodies  Nicotinate And Nicotinamide Metabolism Pathway Diagram
 Nicotinate And Nicotinamide Metabolism Pathway antibodies  Nicotinate And Nicotinamide Metabolism Pathway Diagram
 Purine Catabolism Pathway antibodies  Purine Catabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Pyrimidine Catabolism Pathway antibodies  Pyrimidine Catabolism Pathway Diagram
Diseases associated with anti-NT5E antibodyOrgans/Tissues associated with anti-NT5E antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >23 publications with NT5E and Neoplasms
 Cardiovascular Diseases Antibodies  >16 publications with NT5E and Cardiovascular Diseases
 Inflammation Antibodies  >15 publications with NT5E and Inflammation
 Nervous System Diseases Antibodies  >9 publications with NT5E and Nervous System Diseases
 Breast Neoplasms Antibodies  >8 publications with NT5E and Breast Neoplasms
 Necrosis Antibodies  >7 publications with NT5E and Necrosis
 Pain Antibodies  >7 publications with NT5E and Pain
 Disease Models, Animal Antibodies  >6 publications with NT5E and Disease Models, Animal
 Kidney Diseases Antibodies  >2 publications with NT5E and Kidney Diseases
 Ovarian Neoplasms Antibodies  >2 publications with NT5E and Ovarian Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >24 publications with NT5E and Blood
 Vascular Antibodies  >15 publications with NT5E and Vascular
 Bone Antibodies  >9 publications with NT5E and Bone
 Brain Antibodies  >8 publications with NT5E and Brain
 Muscle Antibodies  >7 publications with NT5E and Muscle
 Ganglia Antibodies  >6 publications with NT5E and Ganglia
 Mouth Antibodies  >6 publications with NT5E and Mouth
 Embryonic Tissue Antibodies  >5 publications with NT5E and Embryonic Tissue
 Bone Marrow Antibodies  >5 publications with NT5E and Bone Marrow
 Skin Antibodies  >4 publications with NT5E and Skin
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